Isolation and Characterization of Pepsin-Soluble Collagen from the Skin of Peru Squid (Dosidicus gigas)

Abstract

Pepsin-soluble collagen (PSC) was isolated from Peru squid (Dosidicus gigas) skin and physicochemical properties of the PSC were determined. The PSC exhibited a maximum absorbance at 220 nm. Sodium dodecyl sulfate polyacrylamide gel electrophoresis suggested the collagen containing α1 and α2 chain was classified as type I collagen. Amino acid composition indicated that the collagen had lower amino acid content than that of mammalian collagen. Denaturation temperature (T_d) of the PSC was 26.8°C. The PSC had relatively high solubility in alkaline condition or NaCl concentrations below 2%. Fourier transform infrared spectroscopy investigations showed the existence of helical arrangements of collagen. The lyophilized collagen had a uniform and regular network structure. These results suggested that Peru squid skin was a potential source of collagen for further research and application.

Keywords:

• Peru squid
• pepsin-soluble collagen
• isolation
• characterization

Acknowledgments

The research was financially supported by the Key Social Development Program of Zhejiang province in China (2010C13009), Aquatic Product Processing Industry Innovation Team Projects of Zhejiang province (2011R09031) and supported by the major science & technology program of Zhejiang province (2011C02003). The authors thank anonymous reviewers for the critical comments and suggestions for the manuscript.