In Vivo Antioxidant Effects of Hydrolysate Derived from Waste Proteins of Mactra veneriformis

Abstract

Mactra veneriformis has been used as seafood and traditional Chinese medicine for thousands of years in China. In the present study, hydrolysate was prepared from waste proteins of M. veneriformis by trypsin treatment. The protective effect of hydrolysate on the defense system against oxidative stress was investigated by estimating the superoxide dismutase (SOD) activity and malondialdehyde (MDA) level in normal mice. The antioxidant activity of hydrolysate was investigated in D-galactose-induced aging mice by estimating the SOD and glutathione peroxidases (GSH-px) activity and MDA level. Amino acid composition and molecular weight distribution were also determined. These investigations suggest that hydrolysate derived from by-products of M. veneriformis possess antioxidant activity and may be attributed to elevation of the activity of antioxidase and enhanced antioxidant defenses.

Keywords:

• Mactra veneriformis
• waste protein
• hydrolysate
• in vivo
• antioxidant activity