Monitoring of Biogenic Amines in Tilapia Flesh (Oreochromis niloticus) by a Simple and Rapid High-Performance Thin-Layer Chromatography Method

ABSTRACT

The monitoring of six biogenic amines (histamine, putrescine, cadaverine, tyramine, spermine, and spermidine) in tilapia (Oreochromis niloticus) flesh held at +4°C and +30°C was accomplished using a straightforward, inexpensive, and trustworthy HPTLC approach. For the three tested concentrations of 50, 100, and 200 mg kg−1, the performance parameters of the method were good in terms of linearity within the range of 25–300 mg kg−1 (R2 ≥ 0.984), precision (RSD ≤ 16%), trueness and accuracy (-20% to +10% range). The shelf life of tilapia was restricted to 18 hours under ambient storage conditions due to its putrescine and cadaverine contents.        

KEYWORDS:

• Biogenic amines
• freshwater fish
• HPTLC
• Oreochromis niloticus
• shelf life

Disclosure statement

No potential conflict of interest was reported by the authors.