Response of juvenile Norway​ spruce (Picea abies [L.] Karst.) to ectomycorrhizal inoculation of perlite-peat substrates in a nursery

ABSTRACT

Nursery growth substrate and ectomycorrhizal symbiosis are important circumstances influencing seedling development. In this study, the effect of pre-sowing inoculation of pure peat and peat+perlite mixtures (2:1 and 1:1, v:v) with an ectomycorrhizal mycelium-bead inoculum prepared in the laboratory and commercial inoculum Ectovit on ectomycorrhiza formation, growth, and foliar nutrients concentration of 1-year-old bare-root Norway spruce seedlings was estimated. Seedlings grown in the substrates inoculated with bead inoculum had significantly larger shoot dry weight and slightly higher other growth parameters than those grown in non-inoculated and Ectovit-inoculated substrates. However, the use of inocula formed neither treatment-specific ectomycorrhizal morphotypes nor higher mycorrhization of roots compared to control. Ectomycorrhizae were most probably formed by naturally occurring fungi; nevertheless, based on the abundance of the morphotypes, any participation of the applied fungi in ectomycorrhiza formation and/or their non-nutritional effects is not excluded. Seedlings grown in peat+perlite (2:1) mixture were slightly higher-developed than those in the two other substrates, but significant effect of the substrates neither on seedling growth nor on ectomycorrhiza formation was detected. In all treatments, sufficient macroelements concentration in needles was found. The results suggest difficulties to reach a complex positive effect of ectomycorrhizal inoculation in operational conditions and its dependence on various circumstances.

KEYWORDS:

• Ectomycorrhizal inoculation
• peat-based substrates
• nutrient content
• Norway spruce
• bare-root seedlings
• nursery

Acknowledgments

The authors thank two anonymous reviewers for their helpful comments, and Mrs. Jana Povaľačová and Mr. Tomáš Trgala for technical assistance.

Additional information

Funding

This work was supported by the Slovak Research and Development Agency [APVV-0744-12].