ABSTRACT
The chloride/nitrate anion exchange assay is one of the most common methods of measuring chloride transport across lipid bilayers. In this methods paper, we provide a step-by-step procedure for using this assay based on ion selective electrodes and highlight the advantages and limitations of this approach in assessing the transmembrane chloride transport capability of anionophores.
Graphical Abstract
![](/cms/asset/1eb5bf16-8d32-4751-8df0-439eec7cf082/gsch_a_1574017_uf0001_oc.jpg)
Acknowledgments
LAJ thanks The University of Sydney for a postgraduate scholarship. PAG thanks the University of Sydney and the ARC (DP180100612) for funding.
Disclosure statement
No potential conflict of interest was reported by the authors.