Sol-gel combustion synthesis of Ag doped CaSiO₃: in vitro bioactivity, antibacterial activity and cytocompatibility studies for biomedical applications

Abstract

CaSiO3 is one of the bioactive calcium silicate materials and known as wollastonite. Herewith, for the first time, we have employed sol–gel combustion method to synthesize Ag-doped CaSiO3 using glycine as a fuel. The sintered powders were characterized by ATR-FTIR, XRD and SEM analysis which proved the existence of characteristic functional groups, pure phase formation and flakes, dense morphology with agglomeration, respectively. TEM-SAED analysis confirms that particles are in nano regime with crystalline nature. SBF immersion reveals that pure and Ag-doped CaSiO3 exhibit the good apatite layer formation for 7 and 14 days. Pure and Ag-doped CaSiO3 were evaluated for their antibacterial behaviour against E. coli and S. aureus which reflects the excellent inhibition of microorganisms. MTT assay proves that the prepared 5 wt-% of Ag-doped CaSiO3 exhibits the excellent cellular metabolic activity with MG-63 cells at different concentrations (50–250 μg ml−1) for 24–48 h.

Keywords:

• Ag doped CaSiO₃
• sol-gel combustion method
• glycine
• bioactivity
• antibacterial activity and cytocompatibility

Acknowledgments

Authors are thankful to the Administration, VIT University Vellore for providing all the necessary facilities and infrastructure to carry out the present research work.