Torulene and torularhodin, protects human prostate stromal cells from hydrogen peroxide-induced oxidative stress damage through the regulation of Bcl-2/Bax mediated apoptosis

Abstract

The current study was designed to elucidate the cytoprotective effects and possible mechanisms of torulene and torularhodin on hydrogen peroxide (H₂O₂)-induced oxidative stress damage in human prostate stromal cells (WPMY-1). After treated with H₂O₂, a notable decrease was appeared in cell viability, yet the decrease was attenuated when cells were pretreated with torulene and torularhodin (0.5–10 μM) as evaluated by WST-1 assay. Pretreatment with these two carotenoids significantly attenuated H₂O₂-induced apoptosis in WPMY-1 cells through the inhibition of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) overproduction, as well as the activation of the activities in catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px). Finally, pretreatment of cells with carotenoids resulted in the regulation of the mRNA and protein expression of Bcl-2 and Bax in H₂O₂-exposed prostate stromal cells. The present results indicate that both torulene and torularhodin can protect human prostate stromal cells from oxidative stress damage via Bcl-2/Bax mediated apoptosis.

Keywords:

• Torulene
• torularhodin
• human prostate stromal cells
• antioxidant effect
• apoptosis

Acknowledgements

This study was supported by National Key Technology R&D Program in the 12th Five year Plan of China (No. 2015BAD16B01, No. 2014BAD04B03), program of “Collaborative innovation center of food safety and quality control in Jiangsu Province”.

Disclosure statement

The authors declare that there are no conflicts of interest.

Additional information

Funding

This study was supported by National Key Technology R&D Program in the 12th Five year Plan of China (No. 2015BAD16B01, No. 2014BAD04B03), program of “Collaborative innovation center of food safety and quality control in Jiangsu Province”.