Antibiofilm and cytotoxic potential of extracellular biosynthesized gold nanoparticles using actinobacteria Amycolatopsis sp. KMN

Abstract

This study intends to biosynthesize gold nanoparticles (AuNPs) using Amycolatopsis sp. KMN and to investigate its potential antibiofilm, cytotoxic and antioxidant activities. The physicochemical characterization of biosynthesize AuNPs was identified by UV-Visible, energy-dispersive X-ray, and Fourier transform infrared spectroscopy, as well as high-resolution transmission electron microscopy, X-ray diffraction, zeta potential, and dynamic light scattering methods. Crystal violet assay and scanning electron microscopy showed that the AuNPs with a particle size of 44.4 nm have a strong antibiofilm activity (at 750 µg/ml concentration) against bacteria strains viz Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853. The result also demonstrated strong cytotoxic activity against two cell lines, MCF-7 and HT-29. The MTT test result displayed that over a period of 48 hr, the IC₅₀ of AuNPs was 600 and 300 µg/ml for MCF-7 and HT-29 cell lines, respectively. The IC₅₀ of AuNPs against DPPH was 46.87 µg/ml. This is the first report that examines Amycolatopsis sp. strain KMN-mediated synthesis of AuNPs is rapid and in situ with antibiofilm and cytotoxicity activities. Moreover, it has the potential for an effective antibiofilm and cytotoxic activity that could be used in future therapeutic applications.

Keywords:

• Actinobacteria
• antibiofilm
• cytotoxic
• gold nanoparticles

Acknowledgments

We appreciate the Islamic Azad University (Qom, Iran) for their helpful support throughout the research project no. 15430507962013, dated September 7, 2018. The authors are also indebted to the Cellular and Molecular Research Center at Qom University of Medical Sciences for their valuable contribution to cytotoxic studies.

Author contributions

Seyyed Soheil Aghaei: conceptualization. Seyyed Soheil Aghaei and Ahmad Ali Pourbabaee: methodology. Mohammad Soleimani: formal analysis. Faezeh Kabiri: investigation and writing—original draft preparation. Seyyed Soheil Aghaei: writing—review and editing. Tahereh Komeili Movahhed: visualization. Seyyed Soheil Aghaei: supervision and project administration. All authors have read and agreed to the published version of the manuscript.

Disclosure statement

No conflict of interest has been declared by the author(s).

Additional information

Funding

This research did not receive any specific grant from funding agencies in the public and commercial sectors.