High cell density continuous fermentation for L-lactic acid production from cane molasses

Abstract

Commercial production of lactic acid (LA) utilizes mostly glucose or lactose coupled with yeast extract (YE) as a supplement. With sugars, nitrogen, and vitamin supplementation being most of the LA production costs, the use of inexpensive molasses, a by-product of the sugar industry, can provide considerable cost savings. There are just a few publications on the production of LA from molasses; consequently, the present investigation was conducted using molasses supplemented with yeast extract. The research was done in a continuous-flow, high-cell-density (HCD) bioreactor with an external membrane microfiltration device for cell recycling. The system, run at 1 L with Lactobacillus delbrueckii NCIM 2025, produced a LA yield of 0.95–0.98 g/g from ∼100 g sugars/L when supplemented with 1 g/L YE. Dilution rates in the range of 0.04–0.36 h⁻¹ resulted in volumetric lactic acid productivities in the range of 4.3–27.6 g/L h, which compares favorably with the highest values recorded in literature, for glucose in the presence of YE, which was as high as 30 g/L. The utilization of cane molasses has a significant impact on the economics of lactic acid production, as measured by a comparison of costs with commercial glucose.

Keywords:

• Cell recycle
• continuous fermentation
• high cell density
• lactic acid
• molasses

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was financially supported by the “Implementation of the “DBT-ICT Center for Energy Biosciences Phase II-BT/EB/ICT-Extension 2012, 05.06.2013” and “Production of Microbial Cocoa Butter Equivalent/DBT-CEB/Farmsow/CCB.”