Bioremoval and tolerance study of sulfamethoxazole using whole cell Trichoderma harzianum isolated from rotten tree bark

Abstract

Antibiotic contamination raises concerns over antibiotic resistance genes (ARGs), which can severely impact the human health and environment. Sulfamethoxazole (SMX) is a widely used antibiotic that is incompletely metabolized in the body. In this study, the research objectives were (1) to isolate the native strain of Trichoderma sp. from the environment and analyze the tolerance toward SMX concentration by evaluating fungal growth, and (2) to investigate the potential of SMX removal by fungi. The potential fungi isolated from rotten tree bark showed 97% similarity to Trichoderma harzianum (Accession no. MH707098.1). The whole cell of fungi was examined in vitro; the strain Trichoderma harzianum BGP115 eliminated 71% of SMX after 7 days, while the white rot fungi Trametes versicolor, demonstrated 90% removal after 10 days. Furthermore, the tolerance of fungal growth toward SMX concentration at 10 mg L⁻¹ was analyzed, which indicated that Trichoderma harzianum BGP115 (the screened strain) exhibited more tolerance toward SMX than Trametes versicolor (the reference strain). The screened fungi isolated from rotted tree bark demonstrated the ability of SMX bioremoval and the potential to be tolerant to high concentrations of SMX.

Keywords:

• Antibiotics
• bioremoval
• fungi
• sulfamethoxazole
• water

Additional information

Funding

This research was supported by National Research Council of Thailand (NRCT) under the capacity of the building and development of new researchers according to the strategic research and innovation for graduate students in 2019 and On-site Laboratory Initiative of Graduate School of Global Environmental Studies, Kyoto University.