Phytochemical analysis and antioxidant activity of Tamarix africana, Arthrocnemum macrostachyum and Suaeda fruticosa, three halophyte species from Algeria

Abstract

Extracts and fractions using six solvents of increasing polarities from Northwest Algeria (Tamarix africana, Arthrocnemum macrostachyum and Suaeda fruticosa) were studied for phytochemical analysis and in vitro antioxidant properties. Methanol and water fractions were found to be the more suitable solvents used for extraction of polyphenolic compounds. Aqueous leaf fraction of T. africana showed the highest content of phenolics (61.06 ± 0.40 mg GAE/g DW) and condensed tannins (118.43 ± 11.79 mg CE/g DW). Dichloromethane stem fraction of T. africana had the highest 2,2-diphenyl-1-picrylhydrazil radical scavenging ability (0.34 ± 0.00 mg/ml). Methanol leaf fraction of the same plant exhibited the highest antioxidant power against the inhibition of β-carotene bleaching, while the maximum total antioxidant capacity was recorded in the leaf extract of S. fruticosa. Phenolic content was not influenced by the species but very affected by the extraction solvent, while antioxidant activities were not influenced by these two parameters. High-performance liquid chromatography with a diode-array detector analysis of methanol and aqueous leaf fractions of T. africana revealed the presence of six phenolic acids; chlorogenic and gallic acids were predominant and 10 flavonoid compounds among which rutin and quercetin-3-O-arabonoside were the major constituents. These findings suggest that these species may be considered as an interesting source of antioxidants.

Graphical Abstract

Keywords:

• Halophytes
• bioactive substances
• solvent affinity
• antioxidant activity
• high-performance liquid chromatography with a diode-array detector (HPLC-DAD) analysis

Acknowledgments

Nabila Belyagoubi-Benhammou sincerely thanks Dr. Angelo Gismondi and Prof. Antonella Canini, Department of Biology, University of Rome Tor Vergata, Italy, for their help in the HPLC-DAD analysis and for their linguistic revision and correction of this manuscript.

Disclosure statement

No potential conflict of interest was reported by the authors.