Association between rs12742784 polymorphism and hip fracture, bone mineral density, and EPHB2 mRNA expression levels in elderly Chinese women

Abstract

Objective: This study aimed to determine the association between rs12742784 polymorphism in the non-coding area and hip fracture, bone mineral density (BMD), and EPHB2 mRNA expression levels in elderly Chinese women.

Methods: We investigated 250 Chinese women (mean age: 63.5 ± 8.3 years) including 123 hip fracture patients and 127 non-fracture controls. All participants underwent clinical examination to meet the inclusion criteria. Lumbar and hip BMD were detected by dual-energy X-ray absorptiometry. rs12742784 polymorphism was determined by restriction fragment length polymorphism and EPHB2 mRNA expression levels were measured by real-time polymerase chain reaction.

Results: Distribution of rs12742784 genotypes agreed with Hardy–Weinberg equilibrium. The frequency of the CT + TT genotype was significantly associated with decreased risk of hip fracture (adjusted odds ratio = 0.57, p < 0.01) after adjusting for age and body mass index, and with increased BMD and EPHB2 mRNA expression levels. The T allele of the rs12742784 single nucleotide polymorphism (SNP) was a protective factor for hip fracture (adjusted odds ratio = 0.56, p < 0.01).

Conclusion: rs12742784 polymorphism was associated with EPHB2 mRNA expression levels, BMD, and hip fracture in Chinese women. The T allele of the rs12742784 SNP was a protective factor for osteoporosis and hip fracture.

摘要

目的：本研究旨在研究中国老年女性非编码区rs12742784多态性与髋部骨折、骨密度（BMD）、EPHB2 mRNA表达水平之间的关系。

方法：调查了250名中国女性（平均年龄：63.5±8.3岁）, 包括123名髋部骨折和127名无骨折的对照。对所有受试者都进行了临床检测确保其符合纳入标准。采用双能x线骨密度仪检测腰椎和髋部骨密度。利用限制性片段长度多态性检测rs12742784多态性, 实时聚合酶链反应检测EPHB2 mRNA表达水平。

结果：rs12742784基因型分布符合Hardy-Weinberg平衡。在调整了年龄和身体质量指数后, CT＋TT基因型的频率与髋部骨折风险的降低显著相关(调整后的优势比＝0.57,p < 0.01), CT＋TT基因型的频率与BMD增加以及EPHB2 mRNA表达水平也显著相关。rs12742784单核苷酸多态性(SNP)的T等位基因是髋部骨折的保护因素(调整后的优势比＝0.56, p<0.01)。

结论：rs12742784多态性与中国女性EPHB2 mRNA表达水平、BMD和髋部骨折有关。rs12742784 SNP的T等位基因是骨质疏松和髋部骨折的保护因素。

Keywords:

• rs12742784
• bone mineral density
• EPHB2 mRNA
• osteoporosis
• hip fracture

Ethical approval

All procedures performed in this study involving human participants were in accordance with the ethical standards of the Institutional Research and Ethical Committee and with the 1964 Helsinki Declaration and its later amendments.

Informed consent

All participants signed a written informed consent and the work was approved by the Ethics and Research Committee of Xiangya Hospital.

Potential conflict of interest

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by Hunan Provincial Natural Science Foundation [2016JJ6165].