Effect of resistance training combined with β-glucan ingestion on bone of ovariectomized mice

Abstract

Objective

This study aimed to evaluate the effects of resistance training (RT) combined with prebiotic β-glucan (BG) ingestion (Saccharomyces cerevisiae) in ovariectomized (OVX) mice.

Methods

Forty-eight mice were divided into six treatments: SHAM group and five OVX groups treated with saline (SAL), alendronate (ALE) (0.1 mg/kg), BG (62.5 mg/kg), RT (climbing a ladder with load) and the combination of BG + RT. All treatments were performed three times per week for 8 weeks starting 38 days after ovariectomy.

Results

OVX effectiveness was confirmed by a reduction in uterus mass (p < 0.05). Femur weight was higher in the SHAM group, followed by the ALE and RT groups with intermediate values and the BG + RT, BG and SAL groups with the lowest values (p < 0.05). The bone mineral density (BMD_DEXA) results were higher for the SHAM and RT groups compared to the other groups (p < 0.05). RT provided similar bone mass and resistance to fracture to ALE and SHAM. We found a similar number of osteocytes in the RT and SHAM groups, which was significantly higher than that found in animals receiving BG (p < 0.05). The BG + RT combination increased calcium bone concentration.

Conclusion

RT promoted benefits on bone health in the OVX model, which were not complemented by the consumption of BG.

摘要

目的:本研究旨在评价阻力训练(RT)联合益生元β-葡聚糖(BG)摄入(酿酒酵母)对去势小鼠(OVX)的影响。

方法:将48只小鼠分为6组:SHAM组和5个OVX组, 分别给予生理盐水(SAL)、阿仑磷酸盐(ALE) (0.1 mg/kg)、BG (62.5 mg/kg)、RT(带负荷爬梯子)和BG + RT联合治疗。所有治疗从卵巢切除术后38天开始, 每周进行3次, 持续8周。

结果:子宫的缩小证实了OVX的有效性(p <0.05)。SHAM组股骨重量较高, ALE组和RT组居中, BG+RT组、BG组和SAL组股骨重量最低(p <0.05)。SHAM组和RT组的骨密度(BMDDEXA)结果高于其他组(p<0.05)。RT组与ALE和SHAM组有相似的骨量和抗骨折性。我们发现RT组与SHAM组骨细胞数量相似, 明显高于接受BG组(p <0.05)。BG + RT组合增加了钙骨浓度。

结论:在OVX模型中, RT促进了骨骼健康的益处, 而BG的摄入并不能补充这一益处。

Keywords:

• Osteoporosis
• resistance training
• β-glucan
• estrogen

Acknowledgements

The authors thank Dr Antônio Carlos Shimano and Dr Roberta Carminati Shimano of the Department of Biomechanics, Medicine and Rehabilitation of the Locomotor System, School of Medicine, USP/RP-SP, Brazil and Geila Santos Carvalho of the Department of Soil Science, Federal University of Lavras – UFLA for their assistance during the experiment. They also thank Filipe Otávio Frederico Marcelino for his assistance in histological analysis. The authors are grateful for the support granted by the National Council for Scientific and Technological Development (CNPq), Higher Educational Personnel Improvement Coordination (CAPES) and the Minas Gerais State Research Foundation (FAPEMIG).

Potential conflict of interest

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by National Council for Scientific and Technological Development (CNPq); Coordination for the Improvement of Higher Education Personnel (CAPES); Research Support Foundation of Minas Gerais (FAPEMIG).