Salvianolic acid B improves diabetic skin wound repair through Pink1/Parkin-mediated mitophagy

Abstract

Diabetic skin wound is a disturbing and rapidly evolving clinical issue. Here, we investigated how salvianolic acid B (Sal B) affected the diabetic wound healing process. Following Sal B administration, histopathological damage was investigated by H&E and Masson staining, and CD34, apoptosis and mitophagy markers were measured by immunofluorescence, immunohistochemistry, and western blotting. Migration, proliferation, and mitochondrial function of high glucose (HG) –induced HMEC-1 cells were measured. The effects of si-Parkin on endothelial cell migration, apoptosis and mitochondrial autophagy were examined. Sal B alleviated inflammatory cell infiltration and promoted angiogenesis in skin wound tissue. Apoptosis and mitophagy were ameliorated by Sal B in diabetic skin wound tissues and HG-induced HMEC-1 cells. Parkin inhibition impaired the migratorypromoted cell apoptosis and inhibited mitophagy of HMEC-1 cells. This finding demonstrated that Sal B promoted diabetic skin wound repair via Pink1/Parkin-mediated mitophagy, improved our understanding of the diabetic wound healing process.

Keywords:

• Skin wound healing
• diabetes
• Salvianolic acid B
• mitophagy
• apoptosis

Disclosure statement

The authors report there are no competing interests to declare.

Data availability statement

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Additional information

Funding

This work was supported by the [Innovation Group Major Research Project of Education Department of Guizhou Province in 2017] under Grant [number Qianjiaohe KY [2017] No.042]; [National Natural Science Foundation of China in 2019] under Grant [number 81960805]; and [Postgraduate Research Fund Project of Guizhou Province in 2020] under Grant [number Qianjiao YJSCXJH(2020)161].