Genome-wide identification and expression of CYP71 gene family in response to low-temperature stress in banana

ABSTRACT

CYP71 belongs to cytochrome P450 (CYP) monooxygenase superfamily, which is functionally diverse and participates in shikimic acid pathway and the secondary metabolism of tryptophan. We identified 58 MaCYP71s and 14 MbCYP71s from the Musa acuminata Colla and Musa balbisiana Colla, and investigated the biological function of the banana CYP71 gene family. Analysis of conserved domain motifs showed that MaCYP71 was more conservative than MbCYP71. Through cluster analysis, MaCYP71 could be divided into 11 subgroups, and MbCYP71 could be divided into 4 subgroups. The expansion of the MaCYP71s might result from the combined actions of tandem and segmental duplications 17% of the MaCYP71 were found to be potentially responsive to low temperature. Exon skipping was the main alternative splicing events of MaCYP71. According to FPKM value and qPCR analysis of MaCYP71 at low temperature, the expressions of MaCYP71A1-4, MaCYP78A6-2, MaCYP78A5-5, and MaCYP78A6-3 at 4°C and 0°C were significantly higher than those at 28°C, which reflected their possible association with response to low-temperature stress. Fifteen members of MaCYP71 family were mainly regulated by miRNA (miR398 and novel_7. 15). MaCYP71s were targeted by 26 lncRNA genes, of which 13 lncRNAs were upregulated in expression at 4°C. From the above studies, it was suggested that MaCYP71 play a vital role in response to low-temperature stress in banana.

KEYWORDS:

• Banana
• CYP71
• gene family analysis
• low-temperature stress
• gene expression

Availability of data and materials

The data that support the findings of this study are openly available in [SRA] at https://dataview.ncbi.nlm.nih.gov/?search=SUB4055531, reference number [SRP148506].

Disclosure statement

No potential conflict of interest was reported by the authors.

Author contribution statement

Zhang Chengyu and Ni Shanshan contributed equally to this manuscript. Lin Yuling, XuHan Xu, Lai Zhongxiong conceived the study and design the experimental study. Li Xiaofang, Wu Hao, Li Qianyu and Wang Xiumei have done the experiments. Shen Xu and Liu Fan analyzed the data. Ni Shanshan written the original draft of this paper. Lin Zhengchun, Zhang Shuting, Nigarish Munir, Cheng Chunzhen revised the paper. All authors have read and approved the final version.

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/14620316.2022.2098179

Additional information

Funding

National Key Research and Development Project (2019YFD1000900), National Modern Agricultural Industrial Technology System (Banana) Special Fund (GARS-31-15), Fujian Province Plateau Science Section construction funding (102/71201801101); National Key Research and Development Program of ChinaNational Key Research and Development Program of China [2019YFD1000900].