Increasing CO₂ concentration impact upon nutrient absorption and removal efficiency of supra intensive shrimp pond wastewater by marine microalgae Tetraselmis chui

Abstract

The objective of this study was to investigate the effect of increasing CO₂ concentration on the growth and the capability of Tetraselmis chui. in removal of nitrate, ammonium and phosphate from shrimp pond wastewater (SPWW). The factorial experimental design was used with the treatment of SPWW percentage in culture medium, namely: 100% SPWW, 75% SPWW + 25% Sea Water (SW) and 75% SW + 25% SPWW coupled with three CO₂ concentration treatments: 390 ppm, 550 ppm and 1000 ppm using CO₂ system. Growth of T. chui. for lengh of cultivation period tended to be higher at treatments of 390 ppm CO₂ and 100% SPWW, however there was a declining growth over period of cultivation for both treatments. The growth rate of T. chui was higher for all percentage of SPWW treatments in culture medium at 390 ppm CO₂ concentration compared to other percentage of SPWW treatments and CO₂ concentration treatments. There was a decreasing of growth rate with increasing CO₂ concentration at 100% SPWW and 75% SPWW + 25% SW in culture medium. Nitrogen removal efficiency and removal rate by T. chui. were strongly affected by CO₂ concentration. However, there was no significant effect of increasing CO₂ concentration to removal efficiency and rate of PO₄ by T. chui.

Keywords:

• microalgae Tetraselmis chui and CO₂ concentration
• nutrient removal
• shrimp pond wastewater
• supra intensive aquaculture technique

Acknowledgment

Authors would like to thank to Research and Development Center of Marine, Coastal and Small Islands, Universitas Hasanuddin who have provided authors with facilities for running the experiment. Also thank to Zaenal and Indah Sari who help us for collecting water sample in the field.

Additional information

Funding

This study was funded by Ministry of Research, Technology and Higher Education, Republic of Indonesia under World Class Research scheme with the contract number: 172/SP2H/LT-DRPM/2019.