GENERATION OF A SPECIFIC POLYCLONAL ANTIBODY WITH HIGH AFFINITY TO ATORVASTATIN AND ITS EMPLOYMENT IN THE DEVELOPMENT OF ELISA FOR DETERMINATION OF ATORVASTATIN IN PLASMA

Abstract

For the first time, a polyclonal antibody with high affinity to atorvastatin (ATR) was generated. The high specificity of the antibody for ATR among its structural analogues and co-administered therapeutic agents was proved. The antibody was employed in the development of enzyme-linked immunosorbent assay for quantitation of ATR in plasma. The assay was validated over a working range of 0.2–5 ng/mL. The intra- and interassay precisions were satisfactory; the coefficients of variations were ≤5%. The accuracy of the method was proved as the mean recovery was 96.4 ± 4.3%. The assay can be used in therapeutic monitoring and pharmacokinetic studies for ATR.

Keywords:

• atorvastatin
• ELISA
• pharmacokinetic studies
• plasma
• polyclonal antibody
• therapeutic monitoring

ACKNOWLEDGMENT

The authors thank King Abdulaziz City for Science and Technology for funding the work (KACS-98-16-AT).

Notes

^a No cross-reactivity.

^b Other competitors are diltiazem, propranolol, losartan, valsartan, and amlodepine.