Skin Regeneration by Hybrid Carboxyl Methyl Cellulose/Calcium Alginate Fibers Electrospun Scaffold

ABSTRACT

This study aimed to investigate on elasticity, degradation, and mechanical strength of sodium carboxyl methyl cellulose (SCMC) and calcium alginate (CA)-based scaffolds and their effect on fibroblasts’ proliferation and adhesion. Using electrospinning technique by employing natural polymers provides a progressive approach in tissue engineering by producing similar structure with adjustable properties. Scanning electron microscope (SEM) was applied to observe the morphological and textural properties. Physico-chemical properties were studied using FTIR, tensile strain, contact angle, in vitro degradation, and water absorption test, and the biological properties were conducted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and SEM observations. The results showed that a bead-free fibers was produced with over 80% porosity. CA resulted in enhancing elasticity, wettability, water uptake absorption, and increasing the rate of biodegradation. Biological assessment revealed that fibroblast cells were adhered and proliferated on SCMC/CA scaffold better comparable with pure SCMC scaffold. The overall findings denoted to the chemical, mechanical, hydrophilicity and biological suitability of SCMC/CA nanofiber scaffolds that make it a promising candidate for further studies toward skin tissue engineering applications.

摘要

本研究旨在研究羧甲基纤维素钠 (SCMC) 和海藻酸钙 (CA) 支架的弹性, 降解和机械强度及其对成纤维细胞增殖和粘附的影响. 利用静电纺丝技术, 利用天然聚合物, 通过生产具有可调节性质的类似结构, 为组织工程提供了一种进步的方法. 用扫描电子显微镜 (SEM) 观察其形貌和织构特性. 通过FTIR, 拉伸应变, 接触角, 体外降解和吸水试验研究了其理化性质, 并通过3- (4,5-二甲基噻唑-2-基) −2,5-二苯基四唑溴化铵 (MTT) 测定和SEM观察对其生物学性质进行了研究. 结果表明, 制备的无珠纤维孔隙率超过80%. 钙有助于提高弹性, 润湿性, 吸水率和生物降解率. 生物学评价显示成纤维细胞在SCMC/CA支架上的粘附和增殖能力优于纯SCMC支架. 总体研究结果表明, SCMC/CA纳米纤维支架具有化学, 机械, 亲水性和生物学适用性, 使其成为皮肤组织工程应用进一步研究的有希望的候选者.

KEYWORDS:

• Scaffold
• Biodegradability
• Electrospinning
• Natural Fibers
• Skin
• Wound Healing

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The authors reported there is no funding associated with the work featured in this article.