ABSTRACT
This study aimed to investigate on elasticity, degradation, and mechanical strength of sodium carboxyl methyl cellulose (SCMC) and calcium alginate (CA)-based scaffolds and their effect on fibroblasts’ proliferation and adhesion. Using electrospinning technique by employing natural polymers provides a progressive approach in tissue engineering by producing similar structure with adjustable properties. Scanning electron microscope (SEM) was applied to observe the morphological and textural properties. Physico-chemical properties were studied using FTIR, tensile strain, contact angle, in vitro degradation, and water absorption test, and the biological properties were conducted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and SEM observations. The results showed that a bead-free fibers was produced with over 80% porosity. CA resulted in enhancing elasticity, wettability, water uptake absorption, and increasing the rate of biodegradation. Biological assessment revealed that fibroblast cells were adhered and proliferated on SCMC/CA scaffold better comparable with pure SCMC scaffold. The overall findings denoted to the chemical, mechanical, hydrophilicity and biological suitability of SCMC/CA nanofiber scaffolds that make it a promising candidate for further studies toward skin tissue engineering applications.
摘要
本研究旨在研究羧甲基纤维素钠 (SCMC) 和海藻酸钙 (CA) 支架的弹性, 降解和机械强度及其对成纤维细胞增殖和粘附的影响. 利用静电纺丝技术, 利用天然聚合物, 通过生产具有可调节性质的类似结构, 为组织工程提供了一种进步的方法. 用扫描电子显微镜 (SEM) 观察其形貌和织构特性. 通过FTIR, 拉伸应变, 接触角, 体外降解和吸水试验研究了其理化性质, 并通过3- (4,5-二甲基噻唑-2-基) −2,5-二苯基四唑溴化铵 (MTT) 测定和SEM观察对其生物学性质进行了研究. 结果表明, 制备的无珠纤维孔隙率超过80%. 钙有助于提高弹性, 润湿性, 吸水率和生物降解率. 生物学评价显示成纤维细胞在SCMC/CA支架上的粘附和增殖能力优于纯SCMC支架. 总体研究结果表明, SCMC/CA纳米纤维支架具有化学, 机械, 亲水性和生物学适用性, 使其成为皮肤组织工程应用进一步研究的有希望的候选者.
Disclosure statement
No potential conflict of interest was reported by the authors.