Chemical and toxicological alterations of zearalenone under ozone treatment

ABSTRACT

Our work mainly studied the elimination of zearalenone (ZEN) from corn flour by ozone treatment. We found four degradation products, after separation and identification by UPLC-ESI-QTOF-MS, named as Compound 1 (m/z 335.18), Compound 2 (m/z 351.19), Compound 3 (m/z 321.19) and Compound 4 (m/z 367.18). Then, one main transformation pathway was predicted on the basis of molecular weight and the reaction mechanism of ozone. We had also detected two of the degradation products by our modified UPLC-MS/MS method in real samples—contaminated corn flour. We screened the toxicity of the parent ZEN compound as well as the ozone induced decomposition products with BALB/c mice to evaluate the safety of our method in vivo. Therefore, aqueous ozone was demonstrated to effectively degrade ZEN and chemical structure alternations of ZEN after ozone treatment were predicted in both pure ZEN and real contaminated-corn flour samples. Considering the presence of the by-products formed, differences in the toxic effects on mice exposed to ZEN before and after the ozone treatment emphasise the significance of further study on the quality and toxicity of real food samples after ozone treatment.

KEYWORDS:

• Zearalenone
• Ozone
• UPLC-ESI-QTOF-MS
• UPLC-MS/MS
• Toxicity tests

Disclosure statement

No potential conflict of interest was reported by the authors.

Supplementary material

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Additional information

Funding

This work was supported by the National Natural Science Foundation of China [No. 31772069], the State Grain Administration [201513006-01-05], the National Grain and Oil Crop Product Quality Safety Risk Assessment [GJFP2018001] and the national first-class discipline program of Food Science and Technology [No.JUFSTR20180303].