ABSTRACT
A UPLC-MS/MS method was developed and validated for the determination of aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2) and ochratoxin A (OTA) in raw coffee samples. Mycotoxins were extracted using a modified QuEChERS method with little sample preparation excluding clean-up and enrichment procedures. Linearity was demonstrated for the five mycotoxins in the range 0.125–20 µg/kg. This method shows negligible matrix effect for individual concentrations, thus allowing the use of an external standard procedure. Limits of quantification (LOQ) ranged from 0.45 to 1 μg/kg. Recoveries between 63% and 89% were achieved. The intra- and inter-day precisions were lower than 15%. The applicability of the method was demonstrated, taking into account fitness for purpose, with simplicity, reliability, low costs and environmental friendliness. The results show that 3 out of 4 samples were contaminated with OTA.
Acknowledgments
The authors gratefully acknowledge “Laboratoire Marocain de l’Agriculture (LABOMAG), Casablanca, Morocco” for technical support.
Disclosure statement
No potential conflict of interest was reported by the author(s).