Comparative study on cupric oxide nanoparticles synthesis in saline buffer versus basic water by Spondias mombin peel extract for biocatalysis

Abstract

The authors herein compared the morphology, homogeneity and biocatalytic (peroxidase) activity of the synthesized cupric oxide nanoparticles (CuO NPs) from Spondias mombin peel extract for the first time in saline buffer and basic H₂O system respectively. The saline buffer-assisted synthesis of CuO NPs produced homogenous and monoclinic shaped nanoparticles having excellent peroxidase activity compared to basic H₂O. The saline buffer-assisted synthesis of CuO NPs was optimized in terms of peel extract concentration, time, and light. The study about elemental compositions, crystallinity, size and shape of CuO NPs (average size ⁓ 45 nm and monoclinic shape in PBS system) were confirmed through EDS, XRD, and SEM techniques. And the completion of CuO NPs synthesis within 5 minutes much shorter than the basic H₂O system was confirmed through UV-visible studies. Finally, the saline buffer-assisted CuO NPs showed 10 times higher peroxidase activity against TMB-H₂O₂ system than basic H₂O-system.

Keywords:

• Biocatalyst
• cupric oxide nanoparticles
• green synthesis
• peroxidase activity
• Spondias mombin peel extract

Conflicts of interest

There are no conflicts to declare.

Additional information

Funding

This work was supported by the Jagannath University Research grant (2018-19) under the title “Green Synthesis of Copper Oxide (II) Nanoparticle Using Plant Extract for Biomedical Application.”