Overview of in vitro digestion methods to evaluate bioaccessibility of lipophilic compounds in foods

ABSTRACT

In vitro digestion models for assessing the bioaccessibility of lipophilic compounds from foodstuffs can either be static or dynamic. Carotenoids are the most widely studied compounds, while there is hardly any information on sterols and fat-soluble vitamins available, nor on the use of dynamic methods. There is a large variability in the parameters of static methods, which makes data comparison difficult. To harmonize digestion conditions and compare bioaccessibility, INFOGEST model has been established. This review assesses the most relevant digestion methods (non-INFOGEST, INFOGEST and dynamic) applied to carotenoids, sterols, and fat-soluble vitamins in food matrices in the last ten years.

KEYWORDS:

• Carotenoids
• sterols
• fat-soluble vitamins
• INFOGEST
• dynamic system
• static model

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No potential conflict of interest was reported by the author(s).

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.

Additional information

Funding

This work is part of the project PID2019-104167RB-I00 funded by the Ministry of Science and Innovation (Spain) under grant MCIN/AEI/10.13039/501100011033. Nerea Faubel holds a grant under INVEST/2022/36 program (Ref. CPI-22-458, Generalitat Valenciana, Spain).