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Original Articles

Critical Assessment of Factors Affecting the Accuracy of the IoB Bitterness Method

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Pages 103-106 | Published online: 06 Feb 2018
 

Abstract

The IoB Method for Bitterness Determination, involving the extraction of iso-α-acids into a solvent, followed by UV spectrophotometric measurement, has been critically appraised. The manner in which samples are shaken has given no cause for concern. However, the use of filters for sample pretreatment, and the losses of iso-α-acids onto glassware are sources of potential disagreement between brewery analysts. The problems arising from high-performance liquid chromatography (HPLC) techniques to quantify iso-α-acids have been outlined, and the application of the IoB Bitterness Method to wort is discussed.

Resumen

El método IoB para la determinación de unidades de amargo, que involucra la extracción de iso-alfa ácidos con solventes, seguido por la medición espectrofotométrica en la region U.V., ha sido valorado críticamente. La manera en la cual las muestras son agitadas no presenta problemas. Sin embargo el uso de filtros para el pretratamiento de la muestra y la pérdida de iso alfa ácidos en el material de vidrio, muestran ser fuentes potenciales de discrepancia entre analistas de la cervecería. Los problemas que surgen de las técnicas HPLC para cuantificar iso alfa ácidos son señalados y la aplicación de el método IoB en mosto es discutido.

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