ABSTRACT
Eight different amber suppressor tRNA (suptRNA) mutations in the nematode Caenorhabditis elegans have been isolated; all are derived from members of the tRNATrp gene family (K. Kondo, B. Makovec, R. H. Waterston, and J. Hodgkin, J. Mol. Biol. 215:7–19, 1990). Genetic assays of suppressor activity suggested that individual tRNA genes were differentially expressed, probably in a tissue- or developmental stage-specific manner. We have now examined the expression of representative members of this gene family both in vitro, using transcription in embryonic cell extracts, and in vivo, by assaying suppression of an amber-mutated lacZ reporter gene in animals carrying different suptRNA mutations. Individual wild-type tRNATrp genes and their amber-suppressing counterparts appear to be transcribed and processed identically in vitro, suggesting that the behavior of suptRNAs should reflect wild-type tRNA expression. The levels of transcription of different suptRNA genes closely parallel the extent of genetic suppression in vivo. The results suggest that differential expression of tRNA genes is most likely at the transcriptional rather than the posttranscriptional level and that 5′ flanking sequences play a role in vitro, and probably in vivo as well. Using suppression of a lacZ(Am) reporter gene as a more direct assay of suptRNA activity in individual cell types, we have again observed differential expression which correlates with genetic and in vitro transcription results. This provides a model system to more extensively study the basis for differential expression of this tRNA gene family.
ACKNOWLEDGMENTS
We thank D. Baillie and members of his lab, especially D. Janke and J. Schein, for help and expertise; E. Stringham and P. Candido for pPZ1; A. Fire, E. Hedgecock, D. Moerman, Don Jones, and our research colleagues in the lab for advice and assistance; two anonymous reviewers for helpful comments; and J. Hodgkin and R. Waterston for continued support. We also thank J. Hodgkin, M. MacMorris, and the Caenorhabditis Genetics Center, supported by the U.S. NIH Division of Research Resources, for some strains of C. elegans.
This work was supported by a grant from NSERC Canada to B.M.H. R. M. Linning held an NSERC Canada postgraduate fellowship.