Ribosomal Protein L23 Activates p53 by Inhibiting MDM2 Function in Response to Ribosomal Perturbation but Not to Translation Inhibition

Abstract

The p53-MDM2 feedback loop is vital for cell growth control and is subjected to multiple regulations in response to various stress signals. Here we report another regulator of this loop. Using an immunoaffinity method, we purified an MDM2-associated protein complex that contains the ribosomal protein L23. L23 interacted with MDM2, forming a complex independent of the 80S ribosome and polysome. The interaction of L23 with MDM2 was enhanced by treatment with actinomycin D but not by gamma-irradiation, leading to p53 activation. This activation was inhibited by small interfering RNA against L23. Ectopic expression of L23 reduced MDM2-mediated p53 ubiquitination and also induced p53 activity and G₁ arrest in p53-proficient U2OS cells but not in p53-deficient Saos-2 cells. These results reveal that L23 is another regulator of the p53-MDM2 feedback regulation.

We thank Yanping Zhang and Karen Vousden for providing L11 plasmids and antibodies, Hunjoo Lee for plasmid preparation, David M. Keller and Jayme Gallegos for critically reading the manuscript, and other members in our laboratory for active discussion. We thank Yanping Zhang and his colleagues for communicating their work prior to publication.

L.D. was supported by an NIH grant (EY10572). This work is supported by grants to H.L. from NIH/NCI (CA095441, CA93614, and CA079721).