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Abstract
The genus Calypogeia belongs to one of the most difficult groups of leafy liverworts, with about 90 species. Nine species of the genus are currently known from Europe: C. azurea, C. integristipula, C. neesiana, C. suecica, C. muelleriana, C. sphagnicola, C. fissa, C. arguta, and C. azorica. Recently, another species, morphologically resembling C. muelleriana but genetically distinct from it, was detected using isozyme markers. In the present study, C. muelleriana and C. sp. nov. were further characterized by 13 and 14 specific inter-simple sequence repeat band patterns, respectively. Nei’s genetic distance between the two species was 0·61. Three species-specific bands were converted into sequence characterized amplified region (SCAR) markers. The first SCAR primer pair amplified a product of 360 and 600 bp in C. muelleriana and C. sp. nov., respectively. The two other SCAR markers were diagnostic for other European Calypogeia species.
Keywords:
This work was financially supported by grant no. N303 344235 from the Polish Ministry of Science and Higher Education. We thank Patrycja Gonera and Beata Pater for help in the laboratory. The authors also thank Alain Vanderpoorten and two anonymous reviewers for helpful comments on an earlier draft, and Robin Stevenson and Paul Bartels for checking the English of this manuscript.
Taxonomic Additions and Changes: Nil.