Comparison of Fructooligosaccharide Utilization by Lactobacillus and Bacteroides Species

Abstract

The utilization of 1-kestose (GF₂) and nystose (GF₃), the main components of fructooligosaccharides (FOS), by Lactobacillus and Bacteroides species was examined. Of seven Lactobacillus and five Bacteroides strains that utilized FOS, L. salivarius, L. rhamnosus, L. casei, and L. gasseri utilized only GF₂, whereas L. acidophilus and all the Bacteroides strains utilized both GF₂ and GF₃. Only the strains able to utilize both GF₂ and GF₃ had β-fructosidase activity in the culture supernatants. The culture supernatants of the Lactobacillus strains had higher β-fructosidase activity for GF₂ than for GF₃, whereas those of the Bacteroides strains had higher activity for GF₃ than for GF₂. Furthermore, β-fructosidase activity of the culture supernatants of the Lactobacillus cells grown in the GF₃ medium was much higher than that of the cells grown in the GF₂ medium, whereas the activity of the culture supernatants of the Bacteroides cells grown in the GF₃ medium was almost the same as that of the cells grown in the GF₂ medium. These results indicate that Lactobacillus species metabolize FOS in a different way from that of Bacteroides species.

• fructooligosaccharides
• 1-kestose
• nystose
• Lactobacillus
• Bacteroides