Quantum dot-based nanosensors for diagnosis via enzyme activity measurement

Abstract

Enzymes are essential in the human body, and the disorder of enzymatic activities has been associated with many different diseases and stages of disease. Luminescent semiconductor nanocrystals, also known as quantum dots (QDs), have garnered great attention in molecular diagnostics. Owing to their superior optical properties, tunable and narrow emissions, stable brightness and long lifetime, QD-based enzyme activity measurement has demonstrated improved detection sensitivity, which is considered particularly valuable for early disease diagnosis. Recent studies have also shown that QD-based nanosensors are capable of probing multiple enzyme activities simultaneously. This review highlights the current development of QD-based nanosensors for enzyme detection. The enzyme–QD hybrid system, equipped with unique electronic, optical and catalytic properties, is envisioned as a potential solution in addressing challenges in diagnostics and therapeutics.

Keywords::

• biosensors
• early disease diagnosis
• enzyme kinetics
• enzyme–nanoparticle conjugates
• nanomedicine

Financial & competing interests disclosure

The authors were supported by the Danish Research Councils (11-116325/FTP), Karen Elise Jensen Foundation, Carlsberg Foundation (Carlsbergfondet), the Aase and Ejnar Danielsens Foundation and the Arvid Nilssons Foundation. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.