Abstract
Long noncoding RNAs (lncRNAs), longer than 200 nucleotides in length, play important roles in the development and progression of various cancers. An increasing number of studies have revealed that lncRNAs function as potential oncogenes or tumor suppressors to influence biological processes, such as cell growth, invasion, migration and apoptosis. Urothelial carcinoma associated 1 (UCA1), an oncogenic lncRNA, was first found in bladder cancer and highly expressed in multiple cancers, including gastric cancer, colorectal cancer, lung cancer and breast cancer. UCA1 promotes tumorigenesis mainly via binding to tumor-suppressive microRNAs (miRNAs), activating several pivotal signaling pathways and alteration of epigenetic and transcriptional regulation. In addition, high expression of UCA1 is related to poor clinicopathological features especially for shorter overall survival, suggesting that UCA1 might be regarded as a prognosis biomarker in human cancers. In the present review, we summarized current studies on UCA1 to explore its prognostic value and underlying regulation mechanisms in the development of multiple cancers in order to provide a glimmer of hope for the prevention and treatment of malignant tumors.
Keywords:
Introduction
Cancer is one of the most common malignant diseases, with high morbidity and mortality, which seriously endanger people’s health.Citation1 The majority of patients are diagnosed in the advanced stage of the disease due to indiscernible early symptoms and lack of efficacious screening methods.Citation2 In the present, there are various modes of treatment for cancer patients, including surgery, radiotherapy, chemotherapy, biotherapy and targeted therapy.Citation3 However, the 5-year survival rate remains dismal.Citation4 Therefore, it is particularly important to identify new early biomarker and potential regulation mechanisms in the development of human cancers to improve the prognosis of patients. Recently, studies have shown that lncRNAs play important roles in tumorigenesis and prognosis via functioning as potential oncogenes or tumor suppressor genes.Citation5–Citation7
Less than 2% of the human genes can encode proteins, and the majority of the genome can be transcribed into non-coding RNAs, which further divided into long non-coding RNA (lncRNAs) and short non-coding RNAs (sncRNAs) according to their length.Citation8,Citation9 lncRNAs, lacking protein-coding capacity, are a class of non-coding RNA which are longer than 200 nucleotides in length and can modulate gene expression at the transcriptional, posttranscriptional, epigenetic and translation levels.Citation10–Citation12 Accumulating studies have found that lncRNAs are abnormal expressed and related to the prognosis in a variety of malignant tumors. Hence, it is necessary to explore whether lncRNAs can be used as prognostic markers and the mechanisms that affect the process of malignant tumors.
Urothelial carcinoma associated 1 (UCA1), a novel vital oncogenic lncRNA and a member of the human endogenous retrovirus H family, is 1,442 bp in length and includes 3 exons and 2 introns.Citation13 It was found that UCA1 is located on chromosome 19p13.12 with a TATA box at its 5ʹ end and a polyA tail at the 3ʹ end.Citation14 According to the tissue expression profiling, UCA1 was ubiquitously expressed in embryonic tissues and not expressed in most normal tissues of adults (except the heart and spleen).Citation15 UCA1 was firstly studied in bladder cancer and most localized in the cytoplasm, which may be crucial for the regulation of protein synthesis process in downstream genes.Citation16,Citation17 In recent years, many reports focus on UCA1 and tumorigenesis, and have shown that UCA1 is highly expressed and exerts oncogenic activity in numerous cancers, such as gastric cancer, colorectal cancer, cervical cancer and melanoma. Several studies further indicated that highly expressed UCA1 might serve as a prognostic marker for cancer patients because it is correlated with advanced TNM stage, positive lymph node metastases, higher cell viability and migration, stronger chemotherapy resistance and shorter overall survival.Citation18–Citation20 In this review, we searched recent studies on UCA1 and summarized the underlying mechanisms and relevant clinicopathological features in human cancers, as shown in and , to explore the prognostic value of UCA1.
Table 1 Functional characterizations of UCA1 in multiple human cancers
Table 2 Clinical significance of UCA1 in various human cancers
UCA1 in human cancers
Accumulating studies have demonstrated that UCA1 is highly expressed in various cancers, and its upregulation promotes cellular functions, such as cell proliferation, migration and invasion, which are closely related to prognosis of patients. The present review generalized the prognostic value and underlying mechanisms of UCA1 in human cancers.
Bladder cancer
UCA1, first studied in bladder cancer, was found to be overexpressed in bladder cancer (BC) cell lines. Previous studies have shown that HMGB1 can act as an inducer of epithelial–mesenchymal transition (EMT) to influence the invasion ability of BC cells.Citation21,Citation22 Xue et al demonstrated overexpression of UCA1 could induce EMT process in two ways, the one is through increasing the levels of zinc finger E-box binding homeobox 1 and 2 (ZEB1/2), another way is by inhibiting tumor suppressor hsa-miR-145 and then increasing the level of fascin homolog 1 (FSCN1) to promote the migration and invasion of bladder cancer cells.Citation23 Luo et al also reported that UCA1 inhibited tumor suppressor miR-143 and then upregulated the expression of HMGB1to promote invasion of bladder cancer cells.Citation24 In addition, Gou et al reported that BMP9 was highly expressed in superficial and infiltrating bladder cancer. Furthermore, the researchers found that increased BMP9 markedly upregulated the expression of UCA1, thereby facilitating the proliferation and migration of BC cell.Citation25 Therefore, these data suggested that UCA1 may enhance BC progression by regulating proliferation and migration ability of cancer cells.
Colorectal cancer
Several studies have found that when compared with noncancerous tissues and normal colorectal cells, UCA1 was upregulated in colorectal cancer (CRC) tissues and cell lines. According to previous reports, lncRNA could act as a competing endogenous RNA (ceRNA) to regulate RNA molecules in cytoplasmic.Citation26–Citation29 Bian et al suggested that amplified UCA1 promoted cell proliferation and tumorigenicity, the potential mechanisms were explored and found that UCA1 sponged miR-204-5p to enhance tumor-promoting genes CREB1, BCL2 and RAB22A expression.Citation30 Ni et al analyzed the relationship between UCA1 and clinicopathological features, and found that expression of UCA1 in CRC was significantly associated with positive lymphatic metastasis and higher tumor stage. Kaplan–Meier analysis results indicated that patients with high UCA1 expression had a significantly poorer prognosis compared to those with low UCA1 expression. Then, the author further reported silencing UCA1 repressed cell proliferation, migration, invasion and promoted apoptosis.Citation31 These studies strongly suggested that increased UCA1 exerted oncogenic effects in CRC.
Gastric cancer
The function and mechanisms of UCA1 were investigated in gastric cancer (GC). Li et al assessed the biological function of UCA1 by overexpression or downregulation of UCA1 in gastric cancer cell lines. Experimental results showed that highly expressed UCA1 enhanced the expression of p-AKT3, p-mTOR and S6K and promoted proliferation, migration and invasion of GC cells, while silencing of UCA1 have the opposite results, indicating UCA1 affected cellular functions in GC partly through activation of PI3K-Akt-mTOR signaling pathway.Citation32 Then, Qin et al similarly indicated UCA1 was overexpressed in gastric cancer tissues and cell lines. The level of miR-182 was downregulated and its target gene TIMP2 that closely related to cell proliferation and apoptosis was significantly increased when knockdown UCA1, which decreased the expression of Bcl-2 while enhanced the level of Bax. Further research revealed the expression of p-PI3K, p-AKT and p-GSK3β, key factors of PI3K/AKT/GSK3β signal pathways, and p-IκBα and p-p65, main factors of NF‐κB signal pathway, was increased when overexpression miR-182.Citation33 In addition, Gong et al indicated that upregulated UCA1 facilitated to cell migration and invasion in GC patients. Mechanistically, the researchers found that UCA1 contributed to GC progression via competitively binding miR-203 to increase the expression of ZEB2.Citation34 Similarly, Gu et al also discovered that UCA1 sponged miR-590-3p to enhance CREB1 expression, which would promote GC progression in vitro and vivo.Citation35 In short, these findings revealed that UCA1 might facilitate to the development of GC.
Hepatocellular carcinoma
Several meta-analyses have concluded that lncRNA UCA1 acted as a predictor of poor overall survival for patients with hepatocellular carcinoma (HCC).Citation36,Citation37 Wang et al showed that upregulation of UCA1 was markablely related with advanced TNM stages, positive metastasis and poorer survival rate. After further evaluation of biological function, the proliferation, migration and invasion capacity of HCC cell was repressed, and cell cycle was arrest in G0/G1 when knockdown UCA1. Subsequently, bioinformatics analysis and luciferase assays revealed that UCA1 could function as an endogenous sponge of miR-216b to promote FGFR1 expression. Depletion of UCA1 repressed the protein expression levels of ERK1/2 and p-ERK1/2, suggesting extracellular signal-regulated kinase (ERK) signaling pathway was involved in HCC progression.Citation38 In addition, Xiao et al similarly demonstrated that silencing UCA1 inhibited HCC cell invasion and metastasis in vitro and in vivo. Moreover, UCA1 could competitively bind to miR-203 to enhance the expression of snail, promoting epithelial–mesenchymal transition and tumor progression.Citation39 Consequently, these data indicated that UCA1 could serve as an oncogene in tumorigenesis.
Breast cancer
Several studies have identified the relationship between UCA1 and chemosensitivity of breast cancer (BC) cells. A report by Liu et al confirmed the strong association between UCA1 and tamoxifen resistance. Mechanistically, inhibition of UCA1 significantly attenuated the proliferation, migration and chemoresistance in the tamoxifen-resistant MCF-7-R and T47D-R cells, possibly due to inhibition of the Wnt/β-catenin pathway activation. Because further research identified that knockdown UCA1 prevented the nuclear translocation of β-catenin.Citation40 Similarly, Wu et al suggested enforced UCA1 markedly reduced tamoxifen sensitivity of MCF-7 cells through activating mTOR signaling pathway.Citation41 In addition, Zhu et al revealed that silencing UCA1 increased the expression of miR-18a and inhibited the expression of Yes-associated protein 1 (YAP1), a regulator of drug sensitivity, which could restore the chemo-sensitivity in trastuzumab-resistant SKBR-3 cells.Citation42 Taken together, these findings demonstrated that UCA1 contributed to regulate the chemo-resistance of BC cells.
Esophageal cancer
Jiao et al reported that UCA1 expression was elevated in esophageal cancer (EC) cells and tissues when compared with esophageal epithelial cell line NE1 and adjacent non-tumor tissues.Citation43 It was reported that lncRNAs can function as sponges of microRNA (miRNA) to regulate its activity.Citation44 The physical interaction between UCA1 and miR-204 was confirmed by RIP assay and luciferase assay. Furthermore, UCA1 acted as a sponge of miR-204 to positively regulate the expression of SOX4 that is a master mediator in tumorigenicity and cancer stemness to promote EC cell proliferation. The study also indicated amplified UCA1 was correlated with advanced TNM stage, higher clinical stage and poor prognosis, suggesting UCA1 might be a prognostic marker for EC patients.
Lung cancer
Recent studies have elucidated the role of UCA1 in lung cancer. According to a study by Nie et al, UCA1 was elevated in human non-small cell lung cancer (NSCLC) tissues. Kaplan–Meier analysis revealed that lung cancer patients with higher UCA1 expression level had poor clinical outcome. Functionally, silencing UCA1 inhibited NSCLC cell proliferation ability via sponging miR-193a-3p to abolish its endogenous suppressive effect on ERBB4.Citation45 Wu et al also reported that UCA1 exerted oncogenes activity and modulated the proliferation and invasion of lung cancer cells. Subsequent mechanism studies demonstrated that UCA1 directly regulated miR-193a to increase the expression of HMGB1 that could function as a carcinogene in tumors.Citation46 Thus, these studies manifested that UCA1 promoted tumorigenesis and its overexpression contributed to the progression of lung cancer.
Prostate cancer
Studies on UCA1 in prostate cancer (PCa) showed that it was significantly evaluated in PCa tissues and cell lines. Chemo- and radio-resistance remain obstacles for the failure of treatment in patients with PCa. Wang et al discovered that upregulation of UCA1 suppressed the activity of miR-204 and positively adjust sirt1 expression. The apoptosis induced by docetaxel was enhanced in docetaxel-resistant 22RV1/DR cells transfected with UCA1 siRNA, sirt1 siRNA and miR-204 mimics, namely UCA1 overexpression increased docetaxel chemoresistance via miR-204/sirt1 axis.Citation47 Subsequently, Ghiam et al found UCA1 was significantly upregulated in prostate cancer radioresistant cells compared with parental cells. Further research suggested that knockdown UCA1 not only could reverse radioresistance, induce cell cycle arrest in G2/M phase but also reduce the level of p-Akt, indicating PI3K/AKT pathway was activated in the development of PCa radioresistance.Citation48 Taken together, UCA1 also could influence the generation of resistance in prostate cancer.
Pancreatic cancer
An increasing number of studies showed that overexpression of UCA1 played important roles in biological processes of pancreatic cancer (PC). Zhang et al elucidated reduction of UCA1 decreased cell growth, migration, invasion ability in vitro and in vivo. Subsequently, rescue experiments manifested that miR-135a partially offset the effect of UCA1 on invasion and migration in PC cells.Citation49 Additionally, a study by Zhou et al reported that UCA1 could bind miR-96 to modulate the expression of FOXO3 that may promote proliferation, metastases and reduce apoptosis of PC cells, indicating that the UCA1/miR-96/FOXO3 axis participated in PC progression.Citation50 Chen et al discovered that UCA1 inhibited the expression of P27 to regulate cell cycle and proliferation of PC cells. Patients with elevated UCA1 expression had shorter overall survival.Citation51 Therefore, these findings suggested that UCA1 participated in the development of PC, and it might be a significant independent predictor of poor survival.
Ovarian cancer
Studies on UCA1 in ovarian cancer (OC) revealed that UCA1 was highly expressed in ovarian tissues compared with the normal tissues, and elevated UCA1 in the patients respond to chemotherapy always had worse survival time.Citation52 Similarly, a recent study indicated that UCA1 was upregulated in paclitaxel-resistant OC cells. Mechanistically, UCA1 acted as a sponge of miR-129 to promote the expression of ABCB1 which contributed to paclitaxel resistance.Citation53 UCA1 was associated with chemo-resistance and overall survival rate, suggesting that it might be a potential prognostic marker in ovarian cancer.
Tongue squamous cell carcinoma
A report by Fang et al demonstrated that UCA1 was highly expressed in tongue squamous cell carcinoma (TSCC) tissues, especially in tissues with lymph node metastasis.Citation54 Consistent with the Fang et al study, Zhang et al further found that TGF-β1 stimulated the expression of UCA1. Mechanistically, the researchers discovered that UCA1 competitively bound to miR-124 to decrease its inhibitory effect on downstream JAG1 and Notch1, which could promote EMT process and invasion of TSCC.Citation55 In general, these results demonstrated that UCA1 acted as an oncogene in TSCC progression.
Melanoma
When compared with normal controls, Tian et al and Wei et al identified that UCA1 expression was obviously elevated in melanoma tissues. Moreover, Tian et al suggested UCA1 might have a correlation with metastasis, and it is highly expressed in melanomas with lymph node metastasis than those at early stage.Citation56 In addition, Wei et al also further confirmed that knockdown UCA1 inhibited cell proliferation and induced G0/G1 cell cycle arrest by directly interacting with miR-507 to affect FOXM1 expression.Citation57 Therefore, UCA1 was involved in cell proliferation, metastasis and cell cycle arrest, it could be considered as a potential therapeutic target.
Thyroid carcinoma
Lu et al have studied the effects of UCA1 on thyroid cancer cells, the researchers showed that highly expressed UCA1 could promote proliferation and invasion, while inhibit apoptosis of thyroid cancer cells.Citation58 A report by Liu et al demonstrated that UCA1 regulated the expression of IGFBP5 by acting as a ceRNA to sponge miR-204 in papillary thyroid carcinoma cells.Citation59 In summary, these studies established a potential role of UCA1 in the development of thyroid carcinoma.
Osteosarcoma
The expression and mechanisms of UCA1 in the osteosarcoma have been studied by Zhu et al, the researchers indicated that knockdown UCA1 inhibited cell growth and migration by regulation of miR-301a and CXCR4. This study further confirmed Wnt and NF-κB signaling pathways were activated when upregulation CXCR4.Citation60 Li et al reported that transcriptional factor HIF‑1α could induce UCA1 expression and promote cell growth of osteosarcoma. Moreover, the author then examined whether UCA1 mediated cell growth and cell cycle arrest through the PTEN/AKT signaling pathway. The results showed that UCA1 suppressed PTEN expression while increased p-AKT expression to regulate cell growth.Citation61 Taken together, UCA1 upregulation is crucial in the pathogenesis of osteosarcoma.
Glioma
Recent studies have elucidated the role of UCA1 in glioma, showing this lncRNA was upregulated in glioma patient tissues and cell lines and associated with poor prognosis. Sun et al confirmed UCA1 promotes the development of glioma by decreasing the expression of tumor suppressor miR-122.Citation62 Similarly, He et al studied the interaction between UCA1 and miRNAs, and found that UCA1 could directly sponge miR-182 to enhance inhibitor of apoptosis-stimulating protein of p53 (IASPP) expression, demonstrating that UCA1/miR-182/IASPP axis participated in glioma progression.Citation63 These findings demonstrated that upregulated UCA1 possessed prognostic value and contributed to the progression of glioma.
Multiple myeloma
There are few published studies concerning the expression of UCA1 in multiple myeloma (MM). In multiple myeloma tissues and cell lines, Zhang et al confirmed that UCA1 expression was markedly evaluated.Citation64 High expression of UCA1 promoted cell proliferation, suppressed cell apoptosis and was associated with poor overall survival in patients with MM. Mechanistically, UCA1 could upregulate oncogene TGF-β expression to promote carcinogenesis. Therefore, UCA1 might be an oncogenic lncRNA in MM, suggesting its potential utilities as a prognostic marker. However, further studies on the role of UCA1 in MM development are warranted.
Oral squamous cell carcinoma
A report by Yang et al found that UCA1 was obviously upregulated in oral squamous cell carcinoma (OSCC) cell lines compared with normal oral cell line. Furthermore, mechanistic analysis revealed that Wnt/β-catenin signaling pathway was activated when UCA1 overexpression, with cell proliferation, migration and invasion promotion and cell apoptosis inhibition.Citation65 Moreover, the connection between UCA1 and cisplatin (CDDP) chemoresistance of oral squamous cell carcinoma was explored by Fang et al. Highly expressed UCA1 existed in CDDP-resistant OSCC cells, it enhanced proliferation, decreased CDDP susceptibility and inhibited CDDP-induced apoptosis of OSCC cells. Molecular mechanisms study further elucidated that UCA1 upregulated SF1 expression by acting as a ceRNA to affect the expression of miR-184.Citation66 Thus, these data revealed that UCA1 knockdown effectively suppressed cell proliferation, migration and invasion and enhanced CDDP susceptibility in OSCC.
Cervical cancer
As for cervical cancer, the present study discovered that UCA1 expression was dramatically increased in cervical cancer tissues. Yan et al discovered that UCA1 directly interacted with miR-206 to increase VEGF expression, which enhanced the migration and invasion capability of cervical cancer cells.Citation67 In addition, Fan et al examined the relationship between UCA1 and radioresistance of cervical cancer cells, the researchers found that UCA1 was significantly elevated in radioresistant cell lines that exhibited increased glycolysis. Further studies found that UCA1 promoted HK2 expression to enhance glucose consumption and lactate production, and ultimately increased the radioresistance of cervical cancer cells.Citation68 Therefore, UCA1 could act as a functional oncogene, and it was expected to regard as a novel prognosis target in cervical cancer.
In summary, these studies indicated that UCA1 was highly expressed in human cancers, and associated with cell proliferation, migration, invasion, apoptosis, chemo- and radio-resistance, and EMT process. In the progress of bladder cancer, colorectal cancer, pancreatic cancer, hepatocellular carcinoma, thyroid carcinoma, etc., these findings suggested UCA1 functioned as a ceRNA to modulate miRNAs and target genes expression, thus promoting tumorigenesis. In addition, in the development of gastric cancer, breast cancer, prostate cancer, osteosarcoma, etc., UCA1 activated several critical signaling pathways, such as Wnt/β-catenin, PI3K/AKT, NF-κB signaling pathway, to enhance biological processes, including ability of cell proliferation, migration, invasion, and chemo- and radio-resistance. Moreover, increased UCA1 usually had poor clinicopathological features and uncomfortable outcome, suggesting UCA1 was closely associated with prognosis, thus, it was expected to serve as a promising predictor for poor prognosis of cancer patients.
Regulating mechanisms of UCA1
Function as a ceRNA
Mounting evidence has demonstrated competing endogenous RNA (ceRNA), an important underlying mechanism in various cancer development, can serve as sponges of miRNAs to modulate its target genes expression.Citation26–Citation28 Bian et al suggested that UCA1 could sponge endogenous miR-204-5p and inhibit its activity, enhancing the expression of CREB1, BCL2 and RAB22A ().Citation30 Another study found that knockdown UCA1 repressed the proliferation, migration and metastasis capacity in esophageal cancer via binding miR-204 to promote the expression of SOX4 that contributes to tumorigenicity ().Citation43 Similarly, Zhou et al reported that UCA1 directly interacted with miR-96 to regulate FOXO3 level, showing that UCA1/miR-96/FOXO3 axis participated in pancreatic cancer progression.Citation50
Figure 1 Regulating mechanisms of UCA1 in multiple human cancers. (A) UCA1 acted as a ceRNA to bind with miR-204-5p to increase the expression of CREB1, BCL2 and RAB22A. (B) UCA1 functioned as a ceRNA to bind with miR-204 to upregulate SOX4 expression level. (C) Increased UCA1 could upregulate N-cadherin and vimentin expression, while E-cadherin, an epithelial marker, was downregulated. (D) UCA1 served as an endogenous sponge of miR-143 to increase the expression of HMGB1 then promote EMT process. (E) Silencing UCA1 could repress the expression of FGFR1to inhibit the activation of ERK signaling pathway. (F) UCA1 could regulate the expression of β-catenin to activate Wnt/β-catenin signaling pathway. (G) The PI3K/AKT signaling pathway was activated when UCA1 was highly expressed.
Involved in signaling pathways
EMT signaling pathway
EMT is an early event of tumor invasion and metastasis, the typical performance of which is downregulation of epithelial cell marker E-cadherin expression and upregulation of stromal cell markers such as N-cadherin, fibronectin and β-catenin.Citation69,Citation70 Xue et al revealed that highly expressed UCA1 in bladder cancer could decrease E-cadherin expression while increase vimentin expression, suggesting UCA1 promoted tumorigenesis through modulating EMT ().Citation23 HMGB1, a member of the high mobility group box subfamily, is an inducer of EMT, Luo et al demonstrated overexpression of UCA1 could competitively bind to miR-143, and thus increase the expression level of HMGB1 and subsequently induce EMT signaling pathway activation ().Citation24
ERK signaling pathway
ERK, which included ERK1 and ERK2, is one of the most common signaling pathways in the MAPK family that is involved in several cellular biological responses such as cell proliferation, differentiation, transformation and apoptosis.Citation71 A study reported that FGFR1 could act as an upstream factor of the MAPK signaling pathway to regulate its cascade.Citation72 Wang et al showed that UCA1 could bind with miR-216b to promote FGFR1 expression, further research found that depletion of UCA1 inhibited the expression of ERK1 and ERK2, indicating high expressed UCA1 could activate ERK signaling pathway ().Citation38
Wnt/β-catenin signaling pathway
The β-catenin protein, widely present in eukaryotic cells, was abnormal activated in tumorigenesis. As shown in study by Liu et al, β-catenin was aggregated and transferred to the nucleus in chemoresistance breast cancer cells (MCF-7-R and T47D-R). In addition, it was found that β-catenin located in the cytoplasm when suppressed lncRNA UCA1, suggesting that UCA1 could contribute to the β-catenin cytonuclear translocation.Citation40 At the same time, in the study of breast cancer, Xiao et al also reported that overexpressed UCA1 upregulated nuclear β-catenin expression level and increased transcription of cyclin D1 and MMP-7 that are downstream genes of β-catenin ().Citation73 These data demonstrated that the Wnt/β-catenin signaling pathway was inhibited when silencing UCA1.
PI3K/AKT pathway
The PI3K/AKT signaling pathway is usually abnormal activated and regulates cell proliferation and progression in many human cancers. Experimental conducted by Li et al and Qin et al revealed that amplified UCA1 enhanced the expression of p-PI3K and p-AKT3, suggesting the PI3K/AKT pathway was abnormal activated ().Citation32,Citation33 Similarly, Ghiam et al also found that silencing UCA1 reduced the expression level of p-Akt that could increase chemosensitivity, suggesting UCA1 could activate the PI3K/AKT signaling pathway to act oncogenic effect.Citation48 These findings indicated that UCA1 may play a pivotal role in the development of various cancers.
Epigenetic and transcriptional regulation
Histone methylation modulation
An accumulation of studies have indicated that UCA1 regulates the transcription of genes by epigenetic modifications. A study found that UCA1 facilitated to hepatocellular tumorigenesis through physically binding to enhancer of EZH2 and suppressed transcription of P27 by histone methylation (H3K27me3) on its promoter, resulting in upregulation of CDK2 that is crucial in cell cycle progression ().Citation74 Lee et al suggested that UCA1 was upregulated when SATB1 depletion, with H3K4 trimethylation (H3K4me3) levels increased and H3K27 trimethylation (H3K27me3) level decreased, these indicated that SATB1 repressed the expression of UCA1 and further suppressed growth and survival of breast cancer cells.Citation75 Taken together, several studies demonstrated that UCA1 manipulated transcriptional regulation through histone methylation on the promoter of genes.
Figure 2 Epigenetic and transcriptional regulation mechanisms of UCA1 in multiple human cancers. (A) UCA1 physically bound to EZH2 and suppressed transcription of P27 by histone methylation (H3K27me3) on the promoter of p27Kip1. (B) Transcription factor SP1 could activate UCA1 expression, and UCA1 physically combined with EZH2 to enhance the expression of cyclin D1. (C) TEAD·SMAD complexes promoted the level of UCA1.
Transcriptional factor and complex
Wang et al showed that transcription factor SP1 directly bound to the core promoter of UCA1 to increase its expression, then UCA1 physically interacted with EZH2 to stimulate the translation of cyclin D1, promoting the transition of gastric cancer cells into G1/S and facilitating to cell proliferation ().Citation76 The Hippo pathway is closely related to the TGF-β- SMAD pathway.Citation77 As a report by Mota et al, a tumor suppressor Merlin was downregulated in breast cancer tissues, resulting in inactivation of Hippo pathway and translocation of YAP/TAZ into the nucleus. The YAP/TAZ in the nucleus not only could bind with the transcription factor TEAD but also interact with the SMAD complex in the TGF-β-SMAD pathway to activate downstream target gene UCA1 that involved in tumorigenesis, cell proliferation, migration and invasion ().Citation78 Similarly, Zhang et al found UCA1 could interact with the mediators MOB1, Lats1 and YAP of the Hippo pathway to form a ribonucleoprotein complex, and increase nuclear localization and stabilization of YAP, promoting cell proliferation and inhibiting apoptosis in pancreatic cancer.Citation79 These studies indicated transcription factors and complexes could regulate the expression of UCA1 by binding with their promoters.
Conclusion and future perspectives
In the present, a growing number of evidence has reported that lncRNAs, regulators of human gene expression, were abnormal expressed and influenced the development of cancers. LncRNA UCA1, a typical oncogene, was observed overexpressed in various cancers. Amplified UCA1 could affect many cellular functions, such as contributing to cell proliferation, migration, invasion, while restraining cell apoptosis in vitro and in vivo. The underlying regulatory mechanisms of UCA1 were explored and involved multiple steps, including competitively binding with miRNAs to regulate related genes expression, abnormal activation of several significant signaling pathways, and alteration of epigenetic and transcriptional regulation. These studies also revealed that UCA1 could be regarded as a prognosis biomarker due to highly expression of it was related to poor clinicopathological features especially for shorter overall survival. However, further studies are needed to elucidate the prognostic value of UCA1 in human cancers.
Although lncRNA has received more and more attention, and further studies are carried out in various tumors, but compared with mRNA and miRNA, the detailed mechanisms about lncRNA in tumorigenesis and development have still not been fully explained. In other words, due to the diversity and complexity of lncRNA mechanisms, the current understanding of lncRNA function is still in primary stage, which requires more research on UCA1 to explore the regulatory mechanisms in carcinogenesis in the future.
In summary, UCA1 can be regarded as an oncogene to influence tumor progression and a promising predictor for poor prognosis for cancer patients, further works will be necessary for facilitating translation of UCA1 from basic research into clinical utility as early as possible.
Disclosure
The authors report no conflicts of interest in this work.
Acknowledgment
This study was funded by Health Commission of Hubei Province Scientific Research Project (WJ2019M256).
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