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Review

Prevalence of extrapulmonary tuberculosis among people living with HIV/AIDS in sub-Saharan Africa: a systemic review and meta-analysis

, &
Pages 225-237 | Published online: 05 Nov 2018

Abstract

Background

The double burden of infectious diseases such as tuberculosis (TB) and HIV disproportionately affects Africa. Our objective was to summarize the prevalence of extrapulmonary TB (EPTB) among people living with HIV/AIDS (PLWHA) in sub-Saharan Africa (SSA).

Methods

We searched PubMed and Google Scholar for studies done on the prevalence of EPTB among PLWHA which published in English from 1990 to 2017. We also searched bibliographic indices and browsed through reference lists of articles and journals. We critically appraised the quality of articles and abstracted the data with Joanna Briggs Institute (JBI) checklists. We used Review Manager Software (version 5.3) and Compressive Meta-analysis Software (version 2.2) for analysis. Finally, we summarized the effect estimate by using random-effects model and explored potential sources of heterogeneity by subgroup and sensitivity analyses. We assessed publication bias by funnel plot and statistical test. Finally, we used PRISMA format for reporting.

Results

We included, in this review, 31 studies that fulfilled our inclusion criteria, with a total population of 28,659. The prevalence estimate of EPTB among PLWHA ranged from 6.4% (95% CI: 3.8, 9.0) to 36.8% (95% CI: 28.6, 45); random-effects pooled prevalence of EPTB among PLWHA was found to be 20% (95% CI: 17, 22; heterogeneity: τ2=0; χ2=509.09, degrees of freedom [df]=30, P<0.00001; I2=94%). No evidence of publication bias was observed (P=0.44 for Egger’s regression analysis and P=0.11 for Begg’s rank correlation analysis).

Conclusion

We can conclude from our analyses that the prevalence of EPTB among PLWHA was high. It is necessary to give emphasis for EPTB, especially when screening TB among PLWHA, widening the spectrum of screening to include all EPTB sites in the countries with high EPTB and/or HIV prevalence in the general population.

Introduction

Tuberculosis (TB) is the ninth leading cause of death worldwide and the leading cause from a single infectious agent that is ranking above HIV/AIDS. The disproportionate burden of HIV and TB infection and disease in Africa at the start of the new millennium continues unabated. In 2016, of all estimated 10.4 million new cases of (25% in Africa) TB that resulted in 1.7 million (44% in Africa) deaths worldwide, Africa though, home to only 13% of the world’s population have been living in Africa. Africa bears 30% of HIV prevalence among TB patients or its co-infection as compared to 1.3% by the Eastern Mediterranean region. HIV prevalence in TB patients or co-infection was low in Eastern Mediterranean region (1.3%), nevertheless, it was 30% in Africa.Citation1

Furthermore, Africa bears 75% of about 1.03 million of TB/HIV co-infection cases that occur each year globally. Furthermore, in Africa, TB is often observed first in HIV-infected patients, and it is the leading cause of death among them.Citation1 In addition, the UNAIDS global report in 2016 showed that sub-Saharan Africa (SSA) takes the lead by contributing the highest burden of HIV: 70% (25.5 million) of 36.7 million people living with HIV, 64% of 1.8 million newly infected, and 73% of 1.0 million deaths.Citation2

Extrapulmonary TB (EPTB) is the TB of organs other than the lungs, eg, pleura, lymph nodes, abdomen, genitourinary tract, skin, joints, bones, and meningeal.Citation3,Citation4 A majority of individuals after exposure control the spread of bacillia in pulmonary site, but in others bacilli disseminate through lymphatic or hematogenous system and subsequently affect single or multiple extrapulmonary sites.Citation5 The prevalence of EPTB among notified TB cases in African region in 2017 was 16%, which is the second highest next to Eastern Mediterranean region (24%), which is more than global prevalence of EPTB (15%). The lowest prevalence was reported from Western Pacific region (8%).Citation1

To mitigate the burden of EPTB/HIV co-infection, the WHO took the initiative. In 2007, it published guidelines on how to diagnose EPTB among HIV-positive people which were based on one culture-positive specimen or histological or strong clinical evidence consistent with active EPTB, followed by a decision by a clinician to treat with a full course of anti-TB treatments.Citation6 In addition, the WHO published an up-to-date policy on collaborative TB/HIV activities in 2012.Citation7 This policy emphasized the importance of surveillance of HIV among TB patients and vice versa in all countries. Furthermore, in 2013, the WHO updated the policy on the diagnosis of TB using GeneXpert Mycobacterium tuberculosis/rifampicin as an initial diagnostic test at point of care. It could replace microscopy and be used for diagnosis of all presumptive pulmonary TB (PTB) patients, pediatrics patients, TB/HIV co-infected patients, and selected specimen of the diagnosis of EPTB patients.Citation8,Citation9

A wide range of estimates for the prevalence of EPTB among people living with HIV/AIDS (PLWHA) have been reported from different SSA countries. For instance, in Ethiopia, more than half, 54.7% co-infection among HIV/AIDS individuals, had EPTB.Citation10 In Cameroon and Ghana, HIV co-infection with EPTB was 37.1% and 45.2%, respectively.Citation11,Citation12 However, an estimate reported on the prevalence of EPTB among PLWHA was inconsistent and unclear.

Therefore, for the dual burden of TB/HIV affecting the Africa, we aimed to summarize and synthesize the prevalence of EPTB among HIV-positive individuals in SSA. This is important for public health policy and planning and development of clinical services that address the needs of EPTB and HIV/AIDS patients.

Methods

Study participants

The study participants included as a sample in reviewed studies could be males and females; all age groups; the time of screening or diagnosing EPTB among HIV had been at the same time (both tested at a time) or after the HIV status is known. The time of reviewed studies performed could be either when HIV/AIDS patients have been in pre-antiretroviral therapy or taking antiretroviral therapy.

Outcome

This review considered studies that include the prevalence estimate of EPTB among PLWHA.

Eligibility criteria

For our review, we included the studies that have been done on PLWHA and reported the proportion of co infection for EPTB and HIV. Included studies were observational study design, which includes cohort (prospective or retrospective), cross-sectional or medical review. Studies that were written only in English, health facilities that could be hospitals, health centers or similar that belong to governmental or nongovernmental, or private, and published between January 1, 1990, and November 30, 2017, were included. The screening or investigations were included irrespective of diagnostic methods. Abstracts without full texts, articles without journal name and/or authors, conference proceeding or presentation, studies with total population of less than 50 people, study with incidence, qualitative study, review, case study, TB/HIV co-infection or PTB/HIV only co-infection, and book chapters were excluded from the final analysis.

Search strategy

A three-stage search strategy was used to identify all relevant published literature. First, an initial search of PubMed database and Google Scholar was made. Second, searching the reference lists or bibliographies of all identified articles was checked manually for articles of interest, and finally gray literature was sought from institutions and government websites. Available electronic journal articles were searched through HINARI. We also searched relevant reference lists and relevant journals manually. The dates of publication covered were January 1, 1990, to November 30, 2017. The search engine used with modification was with initial keywords and Medical Subject Headings (MeSH) term for PubMed database: “Tuberculosis, lymph node” OR “tuberculosis, cardiovascular” OR “tuberculosis, central nervous system” OR “tuberculosis, cutaneous” OR “tuberculosis, endocrine” “tuberculosis, gastrointestinal” OR “tuberculosis, hepatic” OR “tuberculosis, ocular” OR “tuberculosis, oral” OR “tuberculosis, osteoarticular” OR “tuberculosis, pleural” OR “tuberculosis, splenic” OR “tuberculosis, urogenital” OR “tuberculosis” AND “HIV” OR “AIDS” OR “human immunodeficiency virus” OR “acquired immunodeficiency syndrome” OR “co-infection” OR “opportunistic infection” OR “opportunistic” OR “sub Saharan Africa”.

Methods of EPTB screening or diagnosis among PLWHA

WHO or National TB Control Program diagnostic methods of the respective country were used; either combination or individual screening methods were used; either of the following methods were used: microbiologically determined sputum microscopy or sputum culture, PCR, GeneXpert, X-ray, broncho-alveolar lavage, histopathology, and ultrasound. Clinical signs and symptoms and response to treatment in combination with one of the abovementioned diagnostic methods were also used. In addition, methods of HIV screening or diagnosis as per WHO or national HIV screening or diagnosis protocol which mainly either rapid test or ELISA test or both were used.

Methodological quality assessment

All articles selected for inclusion in the review were subjected to a rigorous, independent appraisal by the investigators prior to inclusion in the review using standardized critical appraisal instruments from the Joanna Briggs Institute (JBI).Citation13 Two reviewers independently appraised the studies. Disagreement was resolved by consensus between the two reviewers or through consultation with the third author, if required. The checklists were focused on setting, method of sampling techniques and sample size determination, screening/diagnostic method, and validity and reliability of measurements and data analysis. All relevant studies with optimum score were included and discussed with its limitations and risk of biases and how that might affect the results of this review.

Study selection

After studies were identified, we selected studies by passing through four steps primarily screening through titles and abstracts with over inclusion; second, full-text review for eligibility checkup was performed. Third, methodological critical appraisal was performed, and finally studies having optimum quality were included for systemic review and meta-analysis.

Data collection process: data item and extraction tools

We used a standardized form of JBI for data item and extraction.Citation13 This data extraction form was used to extract the relevant information from appraised articles. A number of variables including study locations or country, years of publications, study periods, study designs, number of patients included in the study, and prevalence of EPTB/HIV co-infection were extracted from all studies included in the review. For this review, we used summary estimate rather than individual patient-level data.

Data analysis and statistical methods

Prevalence of EPTB was calculated as a number of EPTB cases per total number of PLWHA. The prevalence data were identified and abstracted or calculated from the given data from respective studies. In studies, when the standard error (SE) was not reported, we calculated it from the prevalence by using the following formula: SE = √P (1–P)/N and 95% CI = P±1.96 × SE, where P is the prevalence, N is the sample size or included PLWHA.

Meta-analysis was done by using Review Manager Software (version 5.3; the Cochrane Collaboration, 2014) and Compressive Meta-analysis Software (version 2.2). Heterogeneity of studies was assessed using Cochran’s Q (reported as χ2, P-values, and degrees of freedom [df]) and I2 statistics, which described the percentage of total variation between studies that were due to inconsistency rather than chance.Citation14 Unlike Q, I2 is inherently dependent on the number of studies included in the review: its values of 25%, 50%, and 75% show low, moderate, and high degrees of heterogeneity, respectively. We also used inverse variance, random-effects model and proportion for the effect size estimate. Unlike fixed-effects model, random-effects model enabled us to see within and between study variations using Kendall’s τ2.Citation15 Sensitivity analysis and subgroup analysis were also performed. Funnel plot was used to see the possibility of publication bias and statistical tests of Egger’s regression analysis and Begg’s rank correlation analysis.Citation16 We used forest plot to summarize the effect size. Finally, we used PRISMA standard for reporting.Citation17

Results

Study characteristics

In our search, we found 341 records. After the removal of duplicates and initial screening of titles and abstracts, we identified 121 potential full-text articles for eligibility. After screening the full-text articles, we included 31 studies in the review with 28,659 HIV-positive people ().

Figure 1 Study flow.

Notes: The number of studies and the sample size contributed by 12 countries from SSA that had included studies were as follows: ten studies with 2,837 people with HIV/AIDS were from Ethiopia,Citation18Citation27 four each were from Nigeria (2,042)Citation28Citation31 and South Africa (13,929),Citation32Citation35 and three were from Cameroon (2,246).Citation11,Citation36,Citation37 Two each were from Uganda (245)Citation38,Citation39 and Zambia (471),Citation40,Citation41 one each was from Tanzania (175),Citation42 Benin (521),Citation43 Burkina Faso (112),Citation44 Malawi (5,561),Citation45 Kenya (846),Citation46 and Gabon (205)Citation47 ().
Abbreviations: EPTB, extrapulmonary TB; TB, tuberculosis.
Figure 1 Study flow.

Table 1 Characteristics of the studies included in the systemic review in SSA, 2017

Methodological quality assessment of included studies

The methodological quality assessments of studies were assessed by using JBI critical appraisal. As per the standard, the study that had optimum quality that was above the mean for “yes” was included ().

Table 2 Critical appraisal results of studies of EPTB among PLWHA by using JBI prevalence critical appraisal checklist, SSA, 2017

Meta-analysis

Our primary meta-analyses aimed to summarize the prevalence of EPTB among PLWHA.

On meta-analysis, data of 28,659 PLWHA as a sample were used to estimate the proportion of EPTB. The summary effect or pooled proportional estimate of meta-analysis of EPTB among PLWHA was found to be 20% (95% CI: 17, 22; heterogeneity: τ2=0; χ2=509.09, df=30, P<0.00001; I2=94%; ).

Figure 2 Forest plot including all available relevant studies on EPTB among PLWHA in SSA, 2017.

Abbreviations: df, degrees of freedom; EPTB, extrapulmonary tuberculosis; PLWHA, people living with HIV/AIDS; SE, standard error; SSA, sub-Saharan Africa.
Figure 2 Forest plot including all available relevant studies on EPTB among PLWHA in SSA, 2017.

Sensitivity analysis

For the statistical fidelity, sensitivity analysis was performed using Compressive Meta-Analysis Software by removing one study while keeping the others in random-effects model, and by excluding one large study,Citation32 the prevalence estimate of EPTB among PLWHA was not changed, 20% (95% CI: 17, 23; heterogeneity: τ2=0.01; χ2=507.37, df=29, P<0.00001; I2=94%; ).

Table 3 Heterogeneity assessment summary by using sensitivity and subgroup analyses, SSA, 2017

Subgroup analysis

Subgroup analysis was done to find heterogeneity based on age, types of EPTB, study design, subregion of SSA, and countries having two or more studies and the time variation. For instance, by study population age group, inconsistency of the estimate was not removed, the EPTB among PLWHA did not vary by age group when we compared children <15 years,Citation23,Citation28,Citation39 adults ≥15 yearsCitation11,Citation21,Citation46 or ≥18 yearsCitation25,Citation29,Citation33 and all age groups in remaining 21 studies ().

Effect of publication year on estimate

To determine the effect of year of publications on EPTB prevalence estimate among PLWHA, we performed meta-regression using Compressive Meta-analysis (CMA) Software. We observed that publication year increased by a year and the point estimate of EPTB prevalence among PLWHA increased with 0.4% at 95% CI (0.2, 0.6%), P=0.00067 ().

Figure 3 Scatter plot showing the effect of publication year on point estimate of prevalence of EPTB among PLWHA, SSA, 2017.

Abbreviations: EPTB, extrapulmonary tuberculosis; PLWHA, people living with HIV/AIDS; SSA, sub-Saharan Africa.
Figure 3 Scatter plot showing the effect of publication year on point estimate of prevalence of EPTB among PLWHA, SSA, 2017.

Publication bias

We assessed publication bias using CMA Software through funnel plot and regression tests with random-effects model for included studies (). There was no publication bias. In addition, we performed statistical test, and no evidence of publication bias was observed (P=0.44 for Egger’s regression analysis; P=0.11 for Begg’s rank correlation analysis).

Figure 4 Funnel plot for SE by point estimate (A) for included studies (B) and input studies that shown similar pooled estimate.

Abbreviation: SE, standard error.
Figure 4 Funnel plot for SE by point estimate (A) for included studies (B) and input studies that shown similar pooled estimate.

Discussion

In our systematic review and meta-analysis of EPTB among PLWHA, we selected 31 studies with 28,659 HIV/AIDS people. Our main findings showed that there was a high and diverse prevalence of EPTB among PLWHA in SSA which ranged from 6.4% (95% CI: 3.8, 9.0) in NigeriaCitation29 to 36.8% (95% CI: 28.6, 45.0) in Ethiopia.Citation19 The overall pooled estimate prevalence of EPTB among PLWHA was 20% (95% CI: 17, 22; heterogeneity: τ2=0; χ2=509.09, df=30, P<0.00001; I2=94%).

This is high when we compared with the prevalence estimate of EPTB among notified TB cases in Africa. According to the WHO report of 2017, the prevalence of EPTB in Africa and world was 16% and 15%, respectively, among notified TB cases in 2016.Citation1 From the WHO report of 2017, we observed the prevalence of EPTB among notified TB cases for respective sub-Saharan countries, when we compared SSA by the geographical area, the eastern SSA was the lead, followed by Southern SSA, and the western was the least. One-third of the studies (ten of 31 studies) were conducted in Ethiopia, with highest prevalence of EPTB (32%) from SSA in notified TB cases, followed by Malawi 26%, Tanzania 21%, Zambia 18%, Burkina Faso, Kenya and Cameroon each 17%, South Africa 10%, Uganda 8%, and Nigeria 5%.Citation1

However, the pooled prevalence of EPTB among PLWHA in subregions of SSA showed a slight difference with highest from southern SSA, 21% (95% CI: 15, 26); followed by eastern SSA, 20% (95% CI: 16, 23), and finally, western SSA, 19% (95% CI: 13, 24). This might be explained that the prevalence of HIV in population was high for the countries with evidence that included in the synthesis. That is, in 2016, SSA had the highest HIV/AIDS burden by sharing 70% (25.5 million) of 36.7 million people living with HIV, 64% of 1.8 million newly infected, and 73% of 1.0 million deaths worldwide.Citation2 The HIV/AIDS burden varies among subregions of SSA countries as eastern and southern Africa is the region hardest hit by HIV/AIDS with lion share, 53% of 36.7 million PLWHA and western and central Africa shares 17%, making 70% of PLWHA in SSA.Citation2

In SSA, the pooled estimate of prevalence of EPTB among PLWHA by countries having two or more studies in subgroup analysis showed higher when compared with estimated prevalence of EPTB among notified TB cases for Africa, 16%. For instance, in Cameroon from western Africa with three studies, the pooled prevalence was 25% (95% CI: 12, 37), which was more than national estimate 17%, in Ethiopia from eastern with 10 studies was 23% (95% CI: 17, 29) that was less than national estimate of 32%, and South Africa was 17% (95% CI: 14, 20) more than national estimate 10%,Citation1 but comparable with study done in respective countries.

Although the clinical presentations of TB among PLWHA vary by stage of HIV-positive individuals, TB is the common disease among them.Citation48 At an early stage of HIV infection, the ratio of PTB to EPTB is 80:20 and at late stage is 50:50.Citation49 In SSA, TB is often the first manifestation of HIV infection, and it is the leading cause of death among HIV-infected individuals.Citation50 Similarly, HIV infection is one of the most important risk factors associated with an increased risk of latent TB infection progressing to active TB disease. While only 10% of immunocompetent individuals infected with M. tuberculosis go on to develop active disease in their lifetime; 50% of those coinfected with HIV develop active TB.Citation51

To reduce the burden of TB, the WHO’s policy on collaborative TB/HIV activities recommends a combination of measures among HIV-infected individuals. These measures include intensified case finding, isoniazid preventive therapy, and infection control and antiretroviral therapy.Citation7

Although good progress was made in countries of SSA for intensified TB case findings among HIV-positive individuals or vice versa, still more intensified case finding of TB among PLWHA shall be performed. More over, if TB screened among PLWHA, the documentation was low as 18% in Africa, thus, documentation needs more focus in Africa.Citation1 In addition, the SSA that had reviewed studies had not fulfilled the three 90th objectives, which is to indicate that, the 1st 90 means 90% of people who are living with HIV, know their HIV status(positive or negative), the 2nd 90 means 90% of people living with HIV are on ART treatment, and the 3rd 90 means among people using ART, 90% of their virus is suppressed.Citation2

These findings are potentially important from a public health perspective since it suggests that more attention should be paid to screen EPTB among PLWHA, especially in countries with high EPTB in population of SSA (eg, Ethiopia and Malawi) and high HIV prevalence in population (eg, southern African countries).

Our sensitivity analyses showed that our overall results had no difference when we excluded one study with largest sample size.Citation32 The heterogeneity was substantial (I2=94%), and we performed subgroup analysis by age group, types of EPTB, study design, time period, subregion, and countries having two or more studies and observed reduced or zero heterogeneity (). Thus, we explored and investigated the sources of inconsistence and finally performed the pooled estimate. We determined the effect of publication year on effect estimate that also one of the sources of heterogeneity. We expected the clinical variation as EPTB could be varied based on the organ involved, and in the way that the setting at which laboratory investigation performed, by whom and by how many people performed and interpreted. In reviewed studies, there was study design variations. Some studies we reviewed were performed with retrospective review of medical records, this results in underestimating the prevalence of EPTB among PLWHA. Others could be conducted with cross-sectional design in which comes the difficulty to judge which comes first.Citation52 Similarly, at the analysis stage, appropriate analysis was not conducted in some studies that is why we excluded some studies when the prevalence of EPTB was not calculated, or not clear, and/or no data.

Overall, the bias that could possibly occur in some of the individual studies included in our review was selection bias that resulted from primary exposure or outcome measurement as the diagnosis of EPTB is challenging.Citation6 Especially, in HIV-positive individuals, TB is atypical, and some facilities where the studies conducted were at health centers or similar. In these facilities, the required material or profession might not present, and in some studies the HIV statuses of some EPTB patients were unknown.

Second, selection bias could occur at design stage, in which a majority of evidence suggested that 19 out of 31 studies were retrospective in nature. In this case, authors were endorsed to underway studies with entry criteria majorly including those records that had complete data and excluded incomplete that could lead to nonresponse or missed variables or cases even without indicating the proportion of incomplete records.Citation53 Finally, the information bias particularly during data collection time could be committed in some studies included in the review as mentioned earlier, because more than half of the studies involved data abstraction from article-based register without piloting or standardizing data abstraction checklists in addition to poor data handling practice in low-income developing countries.Citation54 Thus, in both cases, the prevalence of EPTB among PLWHA, we found in this review, could have been underestimated. For future study, we recommend that further details on exposures and outcome measurement, samples and sampling techniques, and analysis are required.

However, our study has derived its strengths from its capability of having estimating EPTB among PLWHA in double burdened and leading continent, Africa, although not without limitation. As there were diverse and overlapping diagnostic techniques that had been performed in studies we reviewed, we could not perform the analysis of diagnosis that limited us to compare the prevalence of EPTB among PLWHA by diagnostic techniques. In addition, we reviewed articles written and/or published only in English; data published in other languages were not obtained. We therefore recommend collaboration of researchers from different language backgrounds to do a more exhaustive research in the future.

Conclusion

We can conclude from our analyses that the prevalence of EPTB among PLWHA was high. It is necessary to give emphasis for EPTB rather than assuming as a rare event and focusing only on PTB, specifically, when screening TB among PLWHA, widening the spectrum of screening to include all common EPTB types in countries with high EPTB and/or HIV prevalence in the population.

Disclosure

The authors report no conflicts of interest in this work.

References

  • World Health OrganizationGlobal Tuberculosis ReportGenevaWorld Health Organization2017 Licence: CC BY-NCSA 3.0 IGO2017
  • UNAIDSJoint United Nations Programme on HIV/AIDS (UNAIDS)GenevaUNAIDS2017.
  • Ait-KhalidNAlarconEArmengolRManagement of Tuberculosis: A Guide to Essentials of Good Practice6th ednParis, FranceInternational Union Ageinest Tuberculosis and Lung Disease2010
  • World Health OrganizationTB/HIV Clinical ManualGeneva, SwitzerlandWorld Health Organization2004
  • WaniRSClinical manifestations of pulmonary and extra-pulmonary tuberculosisSouth Sudan Med J2013635256
  • World Health OrganizationDiagnosis and Treatment of Smear-Negative Pulmonary and Extrapulmonary Tuberculosis Among Adults and BlescentsGeneva, SwitzerlandWorld Health Organization2007
  • World Health OrganizationCollaborative TB/HIV Activities Guidelines for National Programmes and Other Stake HoldersGeneva, SwitzerlandWorld Health Organization2012
  • DhedaKRuhwaldMTheronGPeterJYamWCPoint-of-care diagnosis of tuberculosis: Past, present and futureAsian Pacific Soc Respir201218217232
  • World Health OrganizationGlobal Tuberculosis ReportGeneva, SwitzerlandWorld Health Organization2016
  • SinkalaEGraySZuluIClinical and ultrasonographic features of abdominal tuberculosis in HIV positive adults in ZambiaBMC Infect Dis20099445119374757
  • AgborAABignaJJBillongSCFactors associated with death during tuberculosis treatment of patients co-infected with HIV at the Yaoundé Central Hospital, Cameroon: an 8-year hospital-based retrospective cohort study (2006–2013)PLoS One2014912e11521125506830
  • NassikasNYangHForsonAKwartengEKwaraAFactors associated with mortality in extrapulmonary tuberculosis patients at a teaching hospital in GhanaGhana Med J2015494233238
  • Joanna Briggs Institute Reviewers’ ManualThe Systematic Review of Prevalence and Incidence DataSouth Australia2014
  • ThorlundKImbergerGJohnstonBCEvolution of heterogeneity (I2) estimates and their 95% confidence intervals in large meta-analysesPLoS One201277e3947122848355
  • HigginsJPThompsonSGDeeksJJAltmanDGMeasuring inconsistency in meta-analysesBMJ2003327741455756012958120
  • EggerMDavey SmithGSchneiderMMinderCBias in meta-analysis detected by a simple, graphical testBMJ199731571096296349310563
  • MoherDLiberatiATetzlaffJAltmanDGPRISMA GroupPreferred reporting items for systematic reviews and meta-analyses: the PRISMA statementPLoS Med200967e1000097619621072
  • AlemieGAGebreselassieFCommon types of tuberculosis and co-infection with HIV at private health institutions in Ethiopia: a cross sectional studyBMC Public Health20141431932324708793
  • AmareDTuberculosis and HIV co-infection among patients on tuberculosis treatment at Fenote Selam District Hospital, Amhara Regional State, Northwest EthiopiaGlobal J Med Res201515522494618
  • GebreegziabherSBYimerSABjuneGATuberculosis case notification and treatment outcomes in West Gojjam Zone, Northwest Ethiopia: A Five-Year Retrospective StudyJ Tuberc Res2016412333
  • DeribewANegussuNMelakuZDeribeKInvestigation outcomes of tuberculosis suspects in the health centers of Addis Ababa, EthiopiaPLoS One201164e1861421526179
  • EsmaelATsegayeGWubieMEndrisMTuberculosis and human immune deficiency virus co-infection in Debre Markos Referral Hospital in Northwest Ethiopia: a five years retrospective studyJ AIDS Clin Res2013412263267
  • HailuDAbegazWEBelayMChildhood tuberculosis and its treatment outcomes in Addis Ababa: a 5-years retrospective studyBMC Pediatr201414616724581267
  • MekonnenDAwokeDEndalkachewDTuberculosis and human immunodeficiency virus in western Burkina Faso: clinical presentation and clinical evaluationBMC Res Notes2015866667126559922
  • SkogmarSBalchaTTJemalZHDevelopment of a clinical scoring system for assessment of immunosuppression in patients with tuberculosis and HIV infection without access to CD4 cell testing--results from a cross-sectional study in EthiopiaGlob Health Action20147231052311024560255
  • TarekegneDJemalMAtanawTPrevalence of human immunodeficiency virus infection in a cohort of tuberculosis patients at Metema Hospital, Northwest Ethiopia: a 3 years retrospective studyBMC Res Notes2016919219727026410
  • YassinMATakeleLGebresenbetSHIV and tuberculosis coinfection in the southern region of Ethiopia: a prospective epidemiological studyScand J Infect Dis200436967067315370654
  • DanielOJAdejumoOAGidadoMAbdur-RazzaqHAJaiyesimiEOHIV-TB co-infection in children: associated factors and access to HIV services in Lagos, NigerPublic Health Action20155316516926399285
  • AffusimCCKesiemeEAbahVOThe Pattern of presentation and prevalence of tuberculosis in HIV-seropositive patients seen at Benin City, NigeriaInt Scholar Res Network201216
  • AdejumoOADanielOJOtesanyaAFFactors associated with TB/HIV co-infection among drug sensitive tuberculosis patients managed in a secondary health facility in Lagos, NigeriaAfr J Infect Dis2017112758228670643
  • IliyasuZBabashaniMPrevalence and predictors of tuberculosis coinfection among HIV-seropositive patients attending the Aminu Kano Teaching Hospital, northern NigeriaJ Epidemiol2009192818719265273
  • GuptaRKLawnSDBekkerLGCaldwellJKaplanRWoodRImpact of human immunodeficiency virus and CD4 count on tuberculosis diagnosis: analysis of city-wide data from Cape Town, South AfricaInt J Tuberc Lung Dis20131781014102223827024
  • HoffmannCJHoffmannJDKenslerCTuberculosis and hepatic steatosis are prevalent liver pathology findings among HIV-infected patients in South AfricaPLoS One2015102e011781325668620
  • NglaziMDBekkerLGWoodRKaplanRThe impact of HIV status and antiretroviral treatment on TB treatment outcomes of new tuberculosis patients attending co-located TB and ART services in South Africa: a retrospective cohort studyBMC Infect Dis20151553654326584607
  • van der PlasHMeintjesGSchutzCComplications of antiretroviral therapy initiation in hospitalised patients with HIV-associated tuberculosisPLoS One201382e5414523408935
  • NammeLHSolangeDMBertrandMNElvisTAchJHChristopherKExtrapulmonary Tuberculosis and HIV co infection in patients treated for tuberculosis at the Doula Hospital in CameroonAnn Tropical Med Public Health201361100104
  • NjozingNBMiguelSSTihPMHurtigAKKarinHAAssessing the accessibility of HIV care packages among tuberculosis patients in the Northwest Region, CameroonBMC Public Health20101012913920226022
  • OcamaPKatwereMPiloyaTThe spectrum of liver diseases in HIV infected individuals at an HIV treatment clinic in Kampala, UgandaAfr Health Sci20088181219357726
  • WobudeyaELukoyeDLubegaIRMugabeFSekaddeMMusokePEpidemiology of tuberculosis in children in Kampala district, Uganda, 2009-2010; a retrospective cross-sectional studyBMC Public Health201515967100426407719
  • SiddiqiOKGhebremichaelMDangXDiagnosis of central nervous system opportunistic infections in HIV-infected Zambian adultsClin Infect Dis201458121771177724668125
  • SinkalaEGraySZuluIClinical and ultrasonographic features of abdominal tuberculosis in HIV positive adults in ZambiaBMC Infect Dis20099445119374757
  • KamenjuPSaidATuberculosis-HIV co-infection among patients admitted at Muhimbili National Hospital in Dares salaam, TanzaniaTanzania J Health Res20111312531
  • AdeSHarriesADTrébucqANational profile and treatment outcomes of patients with extrapulmonary tuberculosis in BéninPLoS One201494e9560324755603
  • MalkinJEPrazuckTSimonnetFTuberculosis and human immunodeficiency virus in western Burkina Faso: clinical presentation and clinical evaluationInt J Tubercul Lung Dis1999116874
  • FeldackerCTweyaHKeiserOCharacteristics of adults and children diagnosed with tuberculosis in Lilongwe, Malawi: findings from an integrated HIV/TB clinicTrop Med Int Health20121791108111622808948
  • MuttaiHLasersonKFAkelloIAntiretroviral therapy uptake among adult tuberculosis patients newly diagnosed with HIV in Nyanza Province, KenyaPublic Health Action20133428629326393048
  • StolpSMHusonMAJanssenSBeyemeJOGrobuschMPTuberculosis patients hospitalized in the Albert Schweitzer Hospital, Lambaréné, Gabon-a retrospective observational studyClin Microbiol Infect20131911E499E50123826878
  • LeedsILMageeMJKurbatovaEVSite of extrapulmonary tuberculosis is associated with HIV infectionClin Infect Dis2012551758122423123
  • SharmaSKMohanAKadhiravanTHIV-TB co-infection: epidemiology, diagnosis & managementIndian J Med Res2005121455056715817963
  • CorbettELMarstonBChurchyardGJde CockKMTuberculosis in sub-Saharan Africa: opportunities, challenges, and change in the era of antiretroviral treatmentLancet2006367951492693716546541
  • AdeizaMOkpapiJAbbaAHIV-Associated tuberculosis: A sub-Saharan African perspectiveSub-Saharan Afr J Med201411114
  • SongJWChungKCObservational studies: cohort and case-control studiesPlast Reconstr Surg201012662234224220697313
  • BenchimolEISmeethLGuttmannAThe Reporting of studies Conducted using Observational Routinely-collected health Data (RECORD) statementPLoS Med20151210e100188526440803
  • VassarMHolzmannMThe retrospective chart review: important methodological considerationsJ Educ Eval Health Prof201310121824324853