Abstract
L.-K. Kang, S.-P.L. Hwang, G.-C. Gong, H.-J. Lin, P.-C. Chen and J. Chang. 2007. Influences of nitrogen deficiency on the transcript levels of ammonium transporter, nitrate transporter and glutamine synthetase genes in Isochrysis galbana (Isochrysidales, Haptophyta). Phycologia 46: 521–533. DOI: 10.2216/06-44.1
Three DNA fragments belonging to genes involved in nitrogen uptake and assimilation, including a nitrate transporter gene (IgNrt2), an ammonium transporter gene (IgAmt), and a glutamine synthetase gene (IgglnII), were obtained from the marine haptophyte, Isochrysis galbana. Based on alignments of the deduced amino acid sequences, the IgNRT2 fragment shared 47% identity with the Cylindrotheca fusiformis (Bacillariophyceae) nitrate transporter, the IgAMT fragment shared 48% identity with the C. fusiformis ammonium transporter, and the IgGSII fragment shared 61% identity with Skeletonema costatum (Bacillariophyceae) glutamine synthetase. Southern hybridization indicated that both IgNrt2 and IgAmt may have more than one copy in the genome of I. galbana, but only a single copy was detected for IgglnII. The transcript abundances of these three genes under various nitrogen-supply conditions were monitored simultaneously by quantitative real-time polymerase chain reaction (PCR). In the presence of ammonium, both IgNrt2 and IgAmt were severely repressed with mean mRNA concentrations of 0.67 and 3.45 µmol (mol 18S rRNA)−1. IgNrt2 and IgAmt mRNA transcripts increased 41-fold and 8.3-fold, respectively, in the presence of nitrate, and increased 160-fold and 15-fold, respectively, in the nitrogen-depleted condition. The mRNA level of IgglnII was low in cells grown in the presence of ammonium [3.98 µmol (mol 18S rRNA)−1], but mild increases in the range of 2- to 4-fold were observed in cells grown in both nitrate-containing and nitrogen-depleted cultures. Our results demonstrated that the expression patterns of IgNrt2, IgAmt, and IgglnII shared a common trend with their homologous genes in diatoms and green algae under various nitrogen-supply conditions. This group of genes could be used as indicators in natural environments to estimate the degree of nitrogen deficiency and chemical forms of nitrogenous nutrients in use on a species-specific basis.
ACKNOWLEDGMENTS
We thank the Core Facility of the Institute of Cellular and Organismic Biology, Academia Sinica, for assistance in DNA sequencing. This study was supported in part by research grants NSC 92-2313-B-019-050 and 93-2313-B-019-037 from by the National Science Council (R.O.C.) and in part by funds from the Center for Marine Bioscience and Biotechnology, National Taiwan Ocean University.