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Articles

Influences of nitrogen deficiency on the transcript levels of ammonium transporter, nitrate transporter and glutamine synthetase genes in Isochrysis galbana (Isochrysidales, Haptophyta)

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Pages 521-533 | Received 13 Jun 2006, Accepted 02 May 2007, Published online: 22 Apr 2019

REFERENCES

  • Allen A.E., Ward B.B. & Song B.K. 2005. Characterization of diatom (Bacillariophyceae) nitrate reductase genes and their detection in marine phytoplankton communities. Journal of Phycology 41: 95–104.
  • Armbrust E.V., Berges J.A., Bowler C., Green B.R., Martinez D., Putnam N.H., Zhou S., Allen A.E., Apt K.E., Bechner M., Brzezinski M.A., Chaal B.K., Chiovitti A., Davis A.K., Demarest M.S., Detter J.C., Glavina T., Goodstein D., Hadi M.Z., Hellsten U., Hildebrand M., Jenkins B.D., Jurka J., Kapitonov V.V., Kroger N., Lau W.W.Y., Lane T.W., Larimer F.W., Lippmeier J.C., Lucas S., Medina M., Montsant A., Obornik M., Parker M.S., Palenik B., Pazour G.J., Richardson P.M., Rynearson T.A., Saito M.A., Schwartz D.C., Thamatrakoln K., Valentin K., Vardi A., Wilkerson F.P. & Rokhsar D.S. 2004. The genome of the diatom Thalassiosira pseudonana: ecology, evolution and metabolism. Science 306: 79–86.
  • Boehringer M. 1993. The DIG system user's guide for filter hybridization Boehringer Mannheim GmbH Biochemica, Mannheim, Germany. 90 pp.
  • Britto D.T., Siddiqi M.Y., Glass A.D.M. & Kronzucker H.J. 2001. Futile transmembrane NH4+ cycling: a cellular hypothesis to explain ammonium toxicity in plants. Proceedings of the National Academy of Sciences of the United States of America 98. 4255–4258.
  • Chen Q. & Silflow C.D. 1996. Isolation and characterization of glutamine synthetase genes in Chlamydomonas reinhardtii. Plant Physiology 112: 987–996.
  • Chomczynski P. 1992. One-hour downward alkaline capillary transfer for blotting of DNA and RNA. Analytical Biochemistry 201: 134–139.
  • Chung C.C., Hwang S.P.L. & Chang J. 2005. Cooccurrence of ScDSP gene expression, cell death and DNA fragmentation in a marine diatom, Skeletonema costatum. Applied and Environmental Microbiology 71: 8744–8751.
  • Clark C.G. 1992. DNA purification from polysaccharide-rich cells, P.D-3.1-2. In: Protocol in protozoology (Ed. by J.J. Lee & A.T. Soldo), pp. 31–32. Society of Protozoology, Lawrence, Kansas, USA.
  • Collos Y., Descolas-Gros C., Fontugne M., Mortain-Bertrand A., Chretiennot-Dinet M.J. & Frikha M.G. 1993. Chemical, isotopic and enzymatic monitoring of free and enclosed seawater: implications for primary production estimates in incubation bottles. Marine Ecology Progress Series 93: 49–54.
  • Crawford N.M. & Glass A.D.M. 1998. Molecular and physiological aspects of nitrate uptake in plants. Trends in Plant Science 3: 389–395.
  • Davidson K., Flynn K.J. & Cunningham A. 1992. Non—steady state ammonium-limited growth of the marine phytoflagellate, Isochrysis galbana Parke. The New phytologist 122: 433–438.
  • Dugdale R.C. & Wilkerson F.P. 1986. The use of super(15)N to measure nitrogen uptake in eutrophic oceans; experimental considerations. Limnology and Oceanography 31: 673–689.
  • Forde B.G. 2000. Nitrate transporters in plants: structure, function and regulation. Biochimica Et Biophysica Acta-Biomembranes 1465: 219–235.
  • Galvan A. & Fernandez E. 2001. Eukaryotic nitrate and nitrite transporters. Cellular and Molecular Life Sciences 58: 225–233.
  • Gardner W.S., Wynne D.S. & Dunstan W.M. 1976. Simplified procedure for the manual analysis of nitrate in seawater. Marine Chemistry 4: 393–396.
  • Gerendas J., Zhu Z.J., Bendixen R., Ratcliffe R.G. & Sattelmacher B. 1997. Physiological and biochemical processes related to ammonium toxicity in higher plants. Zeitschrift Fur Pflanzenernahrung Und Bodenkunde 160: 239–251.
  • Goldman J.C. & Mccarthy J.J. 1978. Steady state growth and ammonium uptake of a fast-growing marine diatom. Limnology and Oceanography 23: 695–703.
  • Gong G.-C., Liu K.-K. & Pai S.-C. 1995. Prediction of nitrate concentration from two end member mixing in the southern East China Sea. Continental Shelf Research 15: 827–842.
  • Gonzalez-Ballester D., Camargo A. & Fernandez E. 2004. Ammonium transporter genes in Chlamydomonas: the nitrate-specific regulatory gene Nit2 is involved in Amt1;1 expression. Plant Molecular Biology 56: 863–878.
  • Hecky R.E. & Kilham P. 1988. Nutrient limitation of phytoplankton in freshwater and marine environments: a review of recent evidence on the effects of enrichment. Limnology and Oceanography 33: 796–822.
  • Hildebrand M. 2005. Cloning and functional characterization of ammonium transporters from the marine diatom Cylindrotheca fusiformis (Bacillariophyceae). Journal of Phycology 41: 105–113.
  • Hildebrand M. & Dahlin K. 2000. Nitrate transporter genes from the diatom Cylindrotheca fusiformis (Bacillariophyceae): mRNA levels controlled by nitrogen source and by the cell cycle. Journal of Phycology 36: 702–713.
  • Howitt S.M. & Udvardi M.K. 2000. Structure, function and regulation of ammonium transporters in plants. Biochimica Et Biophysica Acta-Biomembranes 1465: 152–170.
  • Inokuchi R., Kuma K., Miyata T. & Okada M. 2002. Nitrogen-assimilating enzymes in land plants and algae: phylogenic and physiological perspectives. Physiologic Plantarum 116: 1–11.
  • Keane T., Creevey C., Pentony M., Naughton T. & Mclnerney J. 2006. Assessment of methods for amino acid matrix selection and their use on empirical data shows that ad hoc assumptions for choice of matrix are not justified. BMC Evolutionary Biology 6: 29.
  • Kilham P. & Hecky R.E. 1988. Comparative ecology of marine and freshwater phytoplankton. Limnology and Oceanography 33: 776–795.
  • Koltermann M., Moroni A., Gazzarini S., Nowara D. & Tischner R. 2003. Cloning, functional expression and expression studies of the nitrate transporter gene from Chlorella sorokiniana (strain 211–8k). Plant Molecular Biology 52: 855–864.
  • La Roche J., Mckay R.M.L. & Boyd P. 1999. Immunological and molecular probes to detect phytoplankton responses to environmental stress in nature. Hydrobiologia 401: 177–198.
  • Lindell D. & Post A.F. 2001. Ecological aspects of ntcA gene expression and its use as an indicator of the nitrogen status of marine Synechococcus spp. Applied and Environmental Microbiology 67: 3340–3349.
  • Matsuda Y. & Colman B. 1995. Characterization of sulfate transport in the green alga Chlorella ellipsoidea. Plant and Cell Physiology 36: 1291–1296.
  • Maurin C. & Le Gal Y. 1997. Isoforms of glutamine synthetase in the marine coccolithophorid Emiliania huxleyi (Prymnesiophyceae). Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology 118: 903–912.
  • Mimura T., Reid R.J. & Smith F.A. 1998. Control of phosphate transport across the plasma membrane of Chara corallina. Journal of Experimental Botany 49: 13–19.
  • Morris A.W. & Riley J.P. 1963. The determination of nitrate in seawater. Analytica Chimica Acta 29: 272–279.
  • Pai S.C., Tsau Y.J. & Yang T.I. 2001. pH and buffering capacity problems involved in the determination of ammonia in saline water using the indophenol blue spectrophotometric method. Analytica Chimica Acta 434: 209–216.
  • Palenik B. & Wood A.M. 1998. Molecular markers of phytoplankton physiological status and their application at the level of individual cells. In: Molecular approaches to the study of the ocean (Ed. by K.E. Cooksey), pp. 188–205. Chapman & Hall, London.
  • Quesada A. & Fernandez E. 1994. Expression of nitrate assimilation related genes in Chlamydomonas reinhardtii. Plant Molecular Biology 24: 185–194.
  • Quesada A., Galvan A., Schnell R.A., Lefebvre P.A. & Fernandez E. 1993. Five nitrate assimilation-related loci are clustered in Chlamydomonas reinhardtii. Molecular and General Genetics 240: 387–394.
  • Quesada A., Galvan A. & Fernandez E. 1994. Identification of nitrate transporter genes in Chlamydomonas reinhardtii. Plant Journal 5: 407–419.
  • Quesada A., Hidalgo J. & Fernandez E. 1998. Three Nrt2 genes are differentially regulated in Chlamydomonas reinhardtii. Molecular and General Genetics 258: 373–377.
  • Robertson D.L. & Alberte R.S. 1996. Isolation and characterization of glutamine synthetase from the marine diatom Skeletonema costatum. Plant Physiology 111: 1169–1175.
  • Robertson D.L., Smith G.J. & Alberte R.S. 1999. Characterization of a cDNA encoding glutamine synthetase from the marine diatom Skeletonema costatum (Bacillariophyceae). Journal of Phycology 35: 786–797.
  • Robertson D.L., Smith G.J. & Alberte R.S. 2001. Glutamine synthetase in marine algae: new surprises from an old enzyme. Journal of Phycology 37: 793–795.
  • Sambrook J. & Russell D.W. 2001. Molecular cloning: a laboratory manual Cold Spring Harbor Laboratory Press, New York, USA.
  • Scanlan D.J., Silman N.J., Donald K.M., Wilson W.H., Carr N.G., Joint I. & Mann N.H. 1997. An immunological approach to detect phosphate stress in populations and single cells of photosynthetic picoplankton. Applied and Environmental Microbiology 63: 2411–2420.
  • Shimogawara K. & Usuda H. 1995. Uptake of inorganic phosphate by suspension-cultured tobacco cells: kinetics and regulation by Pi starvation. Plant and Cell Physiology 36: 341–351.
  • Solorzano L. 1969. Determination of ammonia in natural waters by the phenolhypochlorite method. Limnology and Oceanography 14: 799–801.
  • Sonnhammer E.L.L., Heijne G. & Krogh A. 1998. A hidden Markov model for predicting transmembrane helices in protein sequences. In: Proceedings of the 6th international conference on intelligent systems for molecular biology (Ed. by J.I. Glasgow, T.G. Littlejohn, F. Major, R.H. Lathrop, D. Sankoff & C. Sensen), pp. 175–182. AAAI press, Menlo Park, California, USA.
  • Strickland J.D.H. & Parsons T.R. 1972. A practical handbook of seawater analysis Fisheries Research Board of Canada, Ottawa. 310 pp.
  • Takabayashi M., Wilkerson F.P. & Robertson D. 2005. Response of glutamine synthetase gene transcription and enzyme activity to external nitrogen sources in the diatom Skeletonema costatum (Bacillariophyceae). Journal of Phycology 41: 84–94.
  • Thomas G.H., Mullins J.G.L. & Merrick M. 2000. Membrane topology of the Mep/Amt family of ammonium transporters. Molecular Microbiology 37: 331–344.
  • Thompson J.D., Higgins D.G. & Gibson T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic acids research 22: 4673–4680.
  • von Wiren N., Gazzarrini S., Gojon A. & Frommer W.B. 2000. The molecular physiology of ammonium uptake and retrieval. Current Opinion in Plant Biology 3: 254–261.

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