Differential Circular RNA Expression Profiling During Osteogenic Differentiation in Human Adipose-Derived Stem Cells

Abstract

Aim: Circular RNAs (circRNAs) are essential for stem cell differentiation. This study aimed to investigate their exact mechanism of action in human adipose-derived stem cell (hADSC) osteogenesis. Materials & methods: Isolated hADSCs were cultured in growth medium or osteogenic medium, then total RNA was extracted for circRNA microarray, hierarchical cluster, gene ontology, regulating pathway and circRNA–miRNA–mRNA network analyses. Results: A total of 171 circRNAs were upregulated and 119 were downregulated in induced groups compared with those in noninduced groups. Eight circRNAs, 40 miRNAs and 342 mRNAs were selected to construct a competing circRNA-miRNA-mRNA network. Conclusion: These findings may provide novel insight into altered and specific circRNAs that might function as competing endogenous RNAs in hADSCs during osteogenic differentiation.

Keywords::

• bioinformatics
• circRNA–miRNA–mRNA network
• circular RNA
• hADSCs
• osteogenic differentiation

Supplementary data

To view the supplementary data that accompany this paper please visit the journal website at: www.tandfonline.com/doi/suppl/10.2217/epi-2019-0218

author-contributions

S Guo conceived and designed the experiments. Y Kang collected the samples, performed the experiments and analyzed the data. J Liu, T Zhang, Q Sun and D He contributed equipment, materials and analysis tools.

Financial & competing interest disclosure

The authors have no relevant affiliations orfinancial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

All protocols for human tissue collection and handling were approved by the Research Ethics Committee of the First Affiliated Hospital of China Medical University (approval no: (2014)2014-110-2). We have followed the principles outlined in the Declaration of Helsinki for all human experimental investigations.

Data sharing statement

The data used to support the findings of this study are available from the corresponding author upon request. The authors certify that this manuscript reports original clinical trial data. Individual, de-identified participant data that underlie the results reported in this article (text, tables, figures, and appendices) are available from the corresponding author following publication.