Abstract
Aim: To reveal transcriptome-wide N6-methyladenosine (m6A) methylome of coronary artery disease (CAD). Materials&methods: The m6A levels of RNA from peripheral blood mononuclear cells measured by colorimetry were significantly decreased in CAD cases. Transcriptome-wide m6A methylome profiled by methylated RNA immunoprecipitation sequencing (MeRIP-seq) identified differentially methylated m6A sites within both mRNAs and lncRNAs between CAD and control group. Results: Bioinformatic analysis indicated that differentially methylated genes were involved in the pathogenesis of atherosclerosis. MeRIP-quantitative real-time PCR assay confirmed the reliability of MeRIP-seq data. Finally, the rat carotid artery balloon injury model was performed to confirm the role of m6A demethylase FTO in neointima formation. Conclusion: Our study provided a resource of differentially methylated m6A profile for uncovering m6A biological functions in the pathogenesis of CAD.
Supplementary data
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Acknowledgments
We sincerely thank all patients and healthy volunteers who participated in this study.
Financial & competing interests disclosure
This work was supported by the Natural Science Foundation of Beijing (No.7212081 [to L Wang]); CAMS Innovation Fund for Medical Sciences (CIFMS) (No.2016-I2M-1-009 [to L Wang], 2017-I2M-1-004 [to J Li], 2016-I2M-1-011 [to X Lu], 2016-I2M-2-001 [to S Chen], 2016-I2M-3-018 [to J Chen] and 2017-I2M-1-008 [to J Cao]); the National Natural Science Foundation of China (No. 82070473 [to S Chen], No. 91857118 [to X Lu] and 81600361 [to B Yang]); the High-Tech Research and Development Program of China (863 Plan; No.2012AA02A516 [to L Wang]); and the National Basic Research Program of China (973 Plan; No.2011CB503901 [to L Wang]) from the Ministry of Science and Technology of China. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
No writing assistance was utilized in the production of this manuscript.
Data sharing statement
The data supporting the findings of the present study are available from corresponding author upon reasonable request.