Evaluation of the Functional Importance of Organic Cation Transporters on the Ocular Disposition of Its Intravitreally Injected Substrate in Rabbits

Abstract

Purpose: To evaluate the functional role of organic cation transporters (OCT) and ocular tissue distribution of intravitreally injected OCT substrate tetraethylammonium (TEA) in presence of OCT blocker (quinidine).

Methods: New Zealand albino rabbits of either sex were used. Intravitreal quinidine pretreatment was made 30 min before the administration of TEA. Modulation of vitreous and ocular tissue kinetics of OCT substrate was evaluated with or without blocker pretreatment. Gamma scintigraphy was also performed to visualize the vitreous residence of ^99mTc-labelled TEA in the presence and absence of blocker.

Results: Intravitreally injected quinidine did not significantly alter the ocular disposition of TEA. TEA showed less significant posterior elimination kinetics and slow anterior elimination which resulted in longer residence time of TEA in eye after intravitreal administration.

Conclusions: Intravitreally injected OCT substrates may follow an anterior elimination pathway and prolonged residence time in vitreous humor. The present study shows that OCT may not be active from vitreous-to-blood route in the blood–retinal barrier.

KEYWORDS::

• Intravitreal
• Ocular
• Organic cation transporters
• Pharmacokinetics

Declaration of interest: We thank CSIR for providing Senior Research Fellowship to Dr Nirmal J and All India Institute of Medical Sciences for providing intramural grant (F.6-1/2010-Acad) for this research work. The authors report no conflict of interest. The authors alone are responsible for the content and writing of the paper.