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International Journal of Radiation Biology Volume 92, 2016 - Issue 5
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Research Article

Proteomic insights into the functional basis for the response regulator DrRRA of Deinococcus radiodurans

Liangyan WangKey Laboratory of Chinese Ministry of Agriculture and Zhejiang Province for Nuclear Agricultural Sciences, Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou, Zhejiang, China;
,
Jing HuKey Laboratory of Chinese Ministry of Agriculture and Zhejiang Province for Nuclear Agricultural Sciences, Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou, Zhejiang, China;
,
Mengjia LiuKey Laboratory of Chinese Ministry of Agriculture and Zhejiang Province for Nuclear Agricultural Sciences, Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou, Zhejiang, China;
,
Su YangKey Laboratory of Chinese Ministry of Agriculture and Zhejiang Province for Nuclear Agricultural Sciences, Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou, Zhejiang, China;
,
Ye ZhaoKey Laboratory of Chinese Ministry of Agriculture and Zhejiang Province for Nuclear Agricultural Sciences, Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou, Zhejiang, China;
,
Kaiying ChengKey Laboratory of Chinese Ministry of Agriculture and Zhejiang Province for Nuclear Agricultural Sciences, Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou, Zhejiang, China;
,
Guangzhi XuKey Laboratory of Chinese Ministry of Agriculture and Zhejiang Province for Nuclear Agricultural Sciences, Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou, Zhejiang, China; ;Institute of Agriculture and Food Science, Zhejiang Agriculture and Forest University, Lin’an, Zhejiang, China;
,
Mingfeng LiKey Laboratory of Chinese Ministry of Agriculture and Zhejiang Province for Nuclear Agricultural Sciences, Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou, Zhejiang, China; ;Dongying Environmental Protection Agency, Dongying, Shandong, China
,
Bing TianKey Laboratory of Chinese Ministry of Agriculture and Zhejiang Province for Nuclear Agricultural Sciences, Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou, Zhejiang, China; Correspondence[email protected]; [email protected]
&
Yuejin HuaKey Laboratory of Chinese Ministry of Agriculture and Zhejiang Province for Nuclear Agricultural Sciences, Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou, Zhejiang, China; Correspondence[email protected]; [email protected]
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Pages 273-280 | Received 18 Apr 2015, Accepted 28 Jan 2016, Published online: 07 Mar 2016
 
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Abstract

Purpose To investigate the function basis of the recently discovered response regulator, drRRA (DNA damage response regulator A) in Deinococcus radiodurans, we compared the proteomic profile of the radiation-sensitive drRRA mutant with that of wild-type strain under both non-stress and gamma radiation treatment.

Materials and methods Total proteins of D. radiodurans cells were subjected to two-dimension electrophoresis. Protein spots in 2-Dimension gels were silver stained and scanned. Spots that changed significantly in expression levels were selected for mass spectrometry analysis. Seven genes encoding representative proteins were knocked out for stress resistance analysis.

Results A total of 52 proteins displayed significant expression level changes at least 1.5-fold in the mutant relative to wild-type strain under non-stress conditions, with 31 repressed and 21 induced proteins, which might affect the cell response of D. radiodurans to gamma radiation. The proteins were distributed into functional groups including stress response, metabolism, and function unknown. Disruptions of several altered proteins including DRA0259 (Catalase E) and DR1538 (Osmotically inducible protein C), reduced the antioxidant activity of D. radiodurans.

Conclusion Combined with our previous result of transcriptional profile, we further confirmed that inactivation of DrRRA affects the expression of various stress response systems.

Disclosure statement

The authors report no conflict of interest. The authors alone are responsible for the content and writing of the paper.

Funding information

This work was supported by National Basic Research Program of China (2015CB910600), grants from the National Natural Science Foundation of China (31210103904, 31370102, 31170079, 31570058, 31500656), a special Fund for Agroscientific Research in the Public Interest (201103007), the Natural Science Foundation of Zhejiang Province (LY13C010001), the Public Project of Zhejiang Province (2014C33024), and Zhejiang Provincial Natural Science Foundation for Outstanding Young Scientists (LR16C050002).

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