Grape juice concentrate (G8000™) modulates apoptosis but not oxidative stress following rat colon carcinogenesis induced by azoxymethane

Abstract

Objectives: The aim of this study was to evaluate if grape juice concentrate is able to protect against experimental colon carcinogenesis.

Material and methods: For this purpose, a total of 35 male Wistar rats were randomly distributed into seven groups: G1: SHAM animals receiving only saline; G2: animals receiving 15 mg/kg azoxymethane (AOM); G3: animals receiving 1% grape juice concentrate 2 weeks before the administration of AOM; G4: animals receiving 2% grape juice concentrate 2 weeks before the administration of AOM; G5: animals receiving 1% grape juice concentrate 4 weeks after the last administration of AOM; G6: animals receiving 2% grape juice concentrate 4 weeks after the last administration of AOM; G7: animals receiving only 2% grape juice concentrate.

Results: The group that received 2% grape juice concentrate before induction with AOM showed the decreased expression of Bcl-2 compared to those animals that were induced by AOM (positive control). Regarding Bax, animals that received grape juice at 2% decreased Bax immunoexpression when compared to AOM group. Furthermore, animals that intake grape juice at 1% after induced by AOM decreased Bax immunoexpression as well. 8-OHdGLI did not show significant statistically differences (p > 0.05) among groups.

Conclusion: In summary, our results demonstrate that grape juice is able to modulate rat colon carcinogenesis as a result of induction of apoptosis.

• Azoxymethane
• colon carcinogenesis
• grape juice concentrate
• rat

Acknowledgements

We would like to thank Joaquim Soares de Almeida for preparing the slides for immunohistochemistry and Marcelo de Souza Silva by immunohistochemistry.

Declaration of interest

The authors declare no conflict of interest.

DAR is a recipient of the CNPq fellowship. CTFO and APBG are recipients from CAPES.