Chloroplast based genetic diversity among Chinese grapes genotypes

Abstract

Chloroplast simple sequence repeat (cpSSR) markers previously reported were used to analyze chloroplast diversity of Chinese grapes. Nine cpSSR primer pairs from the chloroplast genomes of citrus and tobacco (Nicotiana tabacum L.) were found useful for analyzing the grapes chloroplast genome (cpDNA). Selected cpSSR primer pairs showed polymorphism among 64 varieties, with polymorphism information content (PIC) values ranging from 0.522 to 0.659, with an average of 0.590. Results depicted that a total number of 43 alleles were detected on nine loci with an average of 4.77 alleles per locus with highest number of six alleles for marker ARCP2 and CCMP10. Gene diversity explained by these cpSSR makers ranged from 4 to 68% with an average of 63.66% per marker. The genetic relationship suggested by UPGMA based dendrogram divided the genotypes into three main clusters and four subgroups, results showed that 2B is the biggest group. Overall Hong Ma Ge and Da LI Mei Gui Xiung 98% similar, while 64-1-19 was the most diverse as compared to rest of genotypes.

Keywords:

• Chloroplast
• genetic diversity
• phylogenetic associations
• vitis vinifera

Disclosure statement

The authors declare that they have no competing interests.

Funding information

This work is supported by grants from the Important National Science and Technology Specific Projects (No. 2012FY110100-3), National Science Foundation of China (NSFC) (No. 31401846) and Fundamental Research Funds for the Central Universities (KJQN201540).