Human transbodies that interfere with the functions of Ebola virus VP35 protein in genome replication and transcription and innate immune antagonism

Watee SeesuayCenter of Research Excellence on Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine Siriraj HospitalMahidol University10700BangkokThailand

Surasak JittavisutthikulCenter of Research Excellence on Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine Siriraj HospitalMahidol University10700BangkokThailand

Nawannaporn Sae-limCenter of Research Excellence on Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine Siriraj HospitalMahidol University10700BangkokThailand

Nitat SookrungCenter of Research Excellence on Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine Siriraj HospitalMahidol University10700BangkokThailand;Department of Research and Development, Faculty of Medicine Siriraj HospitalMahidol University10700BangkokThailand

Yuwaporn SakolvareeCenter of Research Excellence on Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine Siriraj HospitalMahidol University10700BangkokThailand

Wanpen ChaicumpaCenter of Research Excellence on Therapeutic Proteins and Antibody Engineering, Department of Parasitology, Faculty of Medicine Siriraj HospitalMahidol University10700BangkokThailandCorrespondence[email protected]

Pages 1-15 | Received 13 Oct 2017, Accepted 08 Jan 2018, Published online: 21 Mar 2018

Cite this article
https://doi.org/10.1038/s41426-018-0031-3
CrossMark

Full Article
Figures & data
References
Supplemental
Citations
Metrics
Licensing
Reprints & Permissions
View PDF PDF

References

LeungDWPrinsKCBaslerCFAmarasingheGKEbolavirus VP35 is a multifunctional virulence factorVirulence20101 526 53110.4161/viru.1.6.129843061251
PubMed Web of Science ®Google Scholar
MühlbergerELötferingBKlenkHDBeckerSThree of the four nucleocapsid proteins of Marburg virus, NP, VP35, and L, are sufficient to mediate replication and transcription of Marburg virus-specific monocistronic minigenomesJ. Virol.19987287568764110291
PubMed Web of Science ®Google Scholar
NodaTAoyamaKSagaraHKidaHKawaokaYNucleocapsid-like structures of Ebola virus reconstructed using electron tomographyJ. Vet. Med. Sci.20056732532810.1292/jvms.67.325
PubMed Web of Science ®Google Scholar
BaslerCFThe Ebola virus VP35 protein functions as a type I IFN antagonistProc. Natl Acad. Sci. USA200097122891229410.1073/pnas.22039829717334
PubMed Web of Science ®Google Scholar
BaslerCFGarcía-SastreAViruses and the type I interferon antiviral system: induction and evasionInt. Rev. Immunol.20022130533710.1080/08830180213277
PubMedGoogle Scholar
BaslerCFThe Ebola virus VP35 protein inhibits activation of interferon regulatory factor 3J. Virol.2003777945795610.1128/JVI.77.14.7945-7956.2003161945
PubMed Web of Science ®Google Scholar
BaslerCFAmarasingheGKEvasion of interferon responses by Ebola and Marburg virusesJ. Interferon Cytokine Res.20092951152010.1089/jir.2009.00762988466
PubMed Web of Science ®Google Scholar
CárdenasWBEbola virus VP35 protein binds double-stranded RNA and inhibits alpha/beta interferon production induced by RIG-I signalingJ. Virol.2006805168517810.1128/JVI.02199-051472134
PubMed Web of Science ®Google Scholar
YenBMulderLCMartinezOBaslerCFMolecular basis for ebolavirus VP35 suppression of human dendritic cell maturationJ. Virol.201488125001251010.1128/JVI.02163-144248944
PubMed Web of Science ®Google Scholar
PrinsKCCárdenasWBBaslerCFEbola virus protein VP35 impairs the function of interferon regulatory factor-activating kinases IKK-epsilon and TBK-1J. Virol.2009833069307710.1128/JVI.01875-082655579
PubMed Web of Science ®Google Scholar
HallerOKochsGWeberFThe interferon response circuit: induction and suppression by pathogenic virusesVirology200634411913010.1016/j.virol.2005.09.024
PubMed Web of Science ®Google Scholar
HartmanALTownerJSNicholSTA C-terminal basic amino acid motif of Zaire ebolavirus VP35 is essential for type I interferon antagonism and displays high identity with the RNA-binding domain of another interferon antagonist, the NS1 protein of influenza A virusVirology200432817718410.1016/j.virol.2004.07.006
PubMed Web of Science ®Google Scholar
LeungDWStructure of the Ebola VP35 interferon inhibitory domainProc. Natl Acad. Sci. USA200910641141610.1073/pnas.08078541062626716
PubMed Web of Science ®Google Scholar
KimberlinCREbolavirus VP35 uses a bimodal strategy to bind dsRNA for innate immune suppressionProc. Natl Acad. Sci. USA201010731431910.1073/pnas.0910547107
PubMed Web of Science ®Google Scholar
ReidSPCárdenasWBBaslerCFHomo-oligomerization facilitates the interferon-antagonist activity of the ebolavirus VP35 proteinVirology200534117918910.1016/j.virol.2005.06.0443955989
PubMed Web of Science ®Google Scholar
LeungDWAn intrinsically disordered peptide from Ebola virus VP35 controls viral RNA synthesis by modulating nucleoprotein-RNA interactionsCell Rep.20151137638910.1016/j.celrep.2015.03.0344599368
PubMed Web of Science ®Google Scholar
KirchdoerferRNAbelsonDMLiSWoodMRSaphireEOAssembly of the Ebola virus nucleoprotein from a chaperoned VP35 complexCell Rep.20151214014910.1016/j.celrep.2015.06.0034500542
PubMed Web of Science ®Google Scholar
LuthraPJordanDSLeungDWAmarasingheGKBaslerCFEbola virus VP35 interaction with dynein LC8 regulates viral RNA synthesisJ. Virol.2015895148515310.1128/JVI.03652-144403485
PubMed Web of Science ®Google Scholar
PrinsKCBasic residues within the ebolavirus VP35 protein are required for its viral polymerase cofactor functionJ. Virol.201084105811059110.1128/JVI.00925-102950600
PubMed Web of Science ®Google Scholar
LeungDWStructural basis for dsRNA recognition and interferon antagonism by Ebola VP35Nat. Struct. Mol. Biol.20101716517210.1038/nsmb.17652872155
PubMed Web of Science ®Google Scholar
ClarkDVJahrlingPBLawlerJVClinical management of Filovirus-infected patientsViruses201241668168610.3390/v40916683499825
PubMed Web of Science ®Google Scholar
GeisbertTWTreatment of Ebola virus infection with a recombinant inhibitor of factor VIIa/tissue factor: a study in rhesus monkeysLancet20033621953195810.1016/S0140-6736(03)15012-X
PubMed Web of Science ®Google Scholar
WarfieldKLGene-specific countermeasures against Ebola virus based on antisense phosphorodiamidate morpholino oligomersPLoS Pathog.20062e110.1371/journal.ppat.00200011326218
PubMed Web of Science ®Google Scholar
HensleyLERecombinant human activated protein C for the postexposure treatment of Ebola hemorrhagic feverJ. Infect. Dis.2007196S390S39910.1086/520598
PubMed Web of Science ®Google Scholar
GeisbertTWPostexposure protection of non-human primates against a lethal Ebola virus challenge with RNA interference: a proof-of-concept studyLancet20103751896190510.1016/S0140-6736(10)60357-1
PubMed Web of Science ®Google Scholar
WarrenTKProtection against filovirus diseases by a novel broad-spectrum nucleoside analogue BCX4430Nature201450840240510.1038/nature13027
PubMed Web of Science ®Google Scholar
PicazoEGiordanettoFSmall molecule inhibitors of ebola virus infectionDrug Discov. Today20152027728610.1016/j.drudis.2014.12.010
PubMed Web of Science ®Google Scholar
KilgorePEGrabensteinJDSalimAMRybakMTreatment of ebola virus diseasePharmacotherapy201535435310.1002/phar.1545
PubMed Web of Science ®Google Scholar
Henao-RestrepoAMEfficacy and effectiveness of an rVSV-vectored vaccine expressing Ebola surface glycoprotein: interim results from the Guinea ring vaccination cluster-randomised trialLancet201538685786610.1016/S0140-6736(15)61117-5
PubMed Web of Science ®Google Scholar
JahrlingPBGeisbertJBSwearengenJRLarsenTGeisbertTWEbola hemorrhagic fever: evaluation of passive immunotherapy in nonhuman primatesJ. Infect. Dis.2007196S400S40310.1086/520587
PubMed Web of Science ®Google Scholar
MaruyamaTEbola virus can be effectively neutralized by antibody produced in natural human infectionJ. Virol.19997360246030112663
PubMed Web of Science ®Google Scholar
ParrenPWGeisbertTWMaruyamaTJahrlingPBBurtonDRPre- and postexposure prophylaxis of Ebola virus infection in an animal model by passive transfer of a neutralizing human antibodyJ. Virol.2002766408641210.1128/JVI.76.12.6408-6412.2002136210
PubMed Web of Science ®Google Scholar
OswaldWBNeutralizing antibody fails to impact the course of Ebola virus infection in monkeysPLoS Pathog.20073e910.1371/journal.ppat.00300091779296
PubMed Web of Science ®Google Scholar
MarziAProtective efficacy of neutralizing monoclonal antibodies in a nonhuman primate model of Ebola hemorrhagic feverPLoS ONE20127e3619210.1371/journal.pone.00361923338609
PubMed Web of Science ®Google Scholar
QiuXSuccessful treatment of ebola virus-infected cynomolgus macaques with monoclonal antibodiesSci. Transl. Med.20124138ra8110.1126/scitranslmed.3003876
PubMed Web of Science ®Google Scholar
QiuXReversion of advanced Ebola virus disease in nonhuman primates with ZMappNature2014514475310.1038/nature137774214273
PubMed Web of Science ®Google Scholar
OlingerGGJr.Delayed treatment of Ebola virus infection with plant-derived monoclonal antibodies provides protection in rhesus macaquesProc. Natl Acad. Sci. USA2012109180301803510.1073/pnas.12137091093497800
PubMed Web of Science ®Google Scholar
PettittJTherapeutic intervention of Ebola virus infection in rhesus macaques with the MB-003 monoclonal antibody cocktailSci. Transl. Med.20135199ra11310.1126/scitranslmed.3006608
PubMed Web of Science ®Google Scholar
de La VegaMAWongGKobingerGPQiuXThe multiple roles of sGP in Ebola pathogenesisViral Immunol.2015283910.1089/vim.2014.0068
PubMed Web of Science ®Google Scholar
CortiDProtective monotherapy against lethal Ebola virus infection by a potently neutralizing antibodyScience20163511339134210.1126/science.aad5224
PubMed Web of Science ®Google Scholar
ForthalDNFunctions of antibodiesMicrobiol. Spectr.20142AID-0019-201410.1128/microbiolspec.AID-0019-2014
PubMedGoogle Scholar
KristensenMBirchDMørck NielsenHApplications and challenges for use of cell-penetrating peptides as delivery vectors for peptide and protein cargosInt. J. Mol. Sci.201617E18510.3390/ijms17020185
PubMed Web of Science ®Google Scholar
LiuJCell-penetrating peptide-mediated delivery of TALEN proteins via bioconjugation for genome engineeringPLoS ONE20149e8575510.1371/journal.pone.00857553896395
PubMed Web of Science ®Google Scholar
KulkeawKHuman monoclonal ScFv neutralize lethal Thai cobra, Naja kaouthia, neurotoxinJ. Proteom.20097227028210.1016/j.jprot.2008.12.007
PubMed Web of Science ®Google Scholar
LuthraPSunDSilvermanRHHeBActivation of IFN-β expression by a viral mRNA through RNase L and MDA5Proc. Natl Acad. Sci. USA20111082118212310.1073/pnas.10124091083033319
PubMed Web of Science ®Google Scholar
Escudero-PérezBVolchkovaVADolnikOLawrencePVolchkovVEShed GP of Ebola virus triggers immune activation and increased vascular permeabilityPLoS. Pathog.201410e100450910.1371/journal.ppat.10045094239094
PubMed Web of Science ®Google Scholar
RiveraAMessaoudiIPathophysiology of Ebola virus infection: current challenges and future hopesACS Infect. Dis.2015118619710.1021/id5000426
PubMed Web of Science ®Google Scholar
DarlingTLSherwoodLJHayhurstAIntracellular cross-linking of filoviral nucleoproteins with Xintrabodies restricts viral packagingFront. Immunol.20178119710.3389/fimmu.2017.011975623874
PubMed Web of Science ®Google Scholar
GarzaJATaylorABSherwoodLJHartPJHayhurstAUnveiling a drift resistant cryptotope within Marburgvirus nucleoprotein recognized by llama single-domain antibodiesFront. Immunol.20178123410.3389/fimmu.2017.012345630700
PubMed Web of Science ®Google Scholar
KugelmanJREmergence of Ebola virus escape variants in infected nonhuman primates treated with the MB-003 antibody cocktailCell Rep.2015122111212010.1016/j.celrep.2015.08.038
PubMed Web of Science ®Google Scholar
JasenoskyLDNeumannGKawaokaYMinigenome-based reporter system suitable for high-throughput screening of compounds able to inhibit Ebolavirus replication and/or transcriptionAntimicrob. Agents Chemother.2010543007301010.1128/AAC.00138-102897319
PubMed Web of Science ®Google Scholar
EdwardsMRHigh-throughput minigenome system for identifying small-molecule inhibitors of Ebola virus replicationACS Infect. Dis.2015138038710.1021/acsinfecdis.5b000534537067
PubMed Web of Science ®Google Scholar
ZhangYJDingJNZhongHHanJGExploration micromechanism of VP35 IID interaction and recognition dsRNA: A molecular dynamics simulationProteins2017851008102310.1002/prot.25269
PubMed Web of Science ®Google Scholar
The UniProt Consortium.UniProt: the universal protein knowledgebaseNucleic Acids Res.201745D158D16910.1093/nar/gkw1099
PubMed Web of Science ®Google Scholar
RetterIAlthausHHMünchRMüllerWVBASE2, an integrative V gene databaseNucleic Acids Res.200533D671D67410.1093/nar/gki088
PubMed Web of Science ®Google Scholar
RoyAKucukuralAZhangYThe Protein Data BankNucleic Acids Res.20105725738
Google Scholar
KozakovDImproving the physical realism and structural accuracy of protein models by a two-step atomic-level energy minimizationBiophys. J.201712255278
Google Scholar
HospitalAAtomic-level protein structure refinement using fragment-guided molecular dynamics conformation samplingStructure20122812781279
Google Scholar

Share icon
Back to Top

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.

People also read
Recommended articles
Cited by

To cite this article:

Reference style: APA Chicago Harvard

Citation copied to clipboard

Reference styles above use APA (6th edition), Chicago (16th edition) & Harvard (10th edition)

Download citation

Download a citation file in RIS format that can be imported by citation management software including EndNote, ProCite, RefWorks and Reference Manager.

Choose format: RIS BibTex RefWorks Direct Export

Choose options: Citation Citation & abstract Citation & references

Human transbodies that interfere with the functions of Ebola virus VP35 protein in genome replication and transcription and innate immune antagonism

References

Information for

Open access

Opportunities

Help and information

Your download is now in progress and you may close this window

Login or register to access this feature

Human transbodies that interfere with the functions of Ebola virus VP35 protein in genome replication and transcription and innate immune antagonism

References

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date