HPLC Assay of Phospholipase A₂ Activity Using Low-Temperature Derivatization of Fatty Acids

REFERENCES

• Atanasov , V. , V. Bardarov , B. Aleksiev , and M. Mitewa . 2005 . Vipoxin specificity studied by gas chromatographic determination of the enzyme reaction products: Influence of Ca, Mg, and Sr . J. Venom. Anim. Toxins Trop. Dis. 11 : 350 – 359 .
   Google Scholar
• Atanasov , V. N. , D. Danchev , M. Mitewa , and S. Petrova . 2009 . Hemolytic and anticoagulant study of the neurotoxin vipoxin and its components–Basic phospholipase A2 and an acidic inhibitor . Biochemistry (Moscow) 74 : 339 – 345 .
   Web of Science ®Google Scholar
• Dole , V. P. , and H. Meinertz . 1960 . Microdetermination of long-chain fatty acids in plasma and tissues . J. Biol. Chem. 235 : 2595 – 2599 .
   PubMed Web of Science ®Google Scholar
• Durst , H. D. , M. Milano , E. J. Kikta Jr. , S. A. Connelly , and E. Grushka . 1975 . Phenacyl esters of fatty acids via crown ether catalysts for enhanced ultraviolet detection in liquid chromatography . Anal. Chem. 47 : 1797 – 1801 .
   PubMed Web of Science ®Google Scholar
• Elsbach , P. , and J. Weiss . 1991 . Utilization of labeled Escherichia coli as phospholipase substrate . In Methods in Enzymology , ed. E. A. Dennis . San Diego : Academic Press .
   Google Scholar
• Hoffmann , G. E. , and W. G. Guder . 1989 . Serum phospholipase: Regulatory and patophysiological aspects . Klin. Wochenschr. 67 : 144 – 148 .
   PubMedGoogle Scholar
• Kaiser , E. , P. Chiba , and K. Zaky . 1990 . Phospholipases in biology and medicine . Clin. Biochem. 23 : 349 – 370 .
   PubMed Web of Science ®Google Scholar
• Kline , W. F. , D. P. Enagonio , D. J. Reeder , and W. E. May . 1982 . Liquid chromatographic determination of valproic acid in human serum . J. Liq. Chromatogr. 5 : 1697 – 1709 .
   Web of Science ®Google Scholar
• Kudo , I. , and M. Murakami . 2002 . Phospholipase A2 enzymes . Prostag. Oth. Lipid M. 68–69 : 3 – 58
   PubMed Web of Science ®Google Scholar
• Miller , A. A. , E. Kurschel , R. Osieva , and C. G. Schmidt . 1987 . Clinical pharmacology of sodium butyrate in patients with acute leukemia . Eur. J. Cancer Clin. Oncol. 23 : 1283 – 1287 .
   PubMedGoogle Scholar
• Mobilio , D. , and L. A. Marshall . 1989 . Recent advances in the design and evaluation of inhibitors of PLA2 . Ann. Rep. Med. Chem. 24 : 157 – 166 .
   Web of Science ®Google Scholar
• Mohamed Shakir , K. M. 1981 . Phospholipase A2 activity of post-heparin plasma: A rapid and sensitive assay and partial characterization . Anal. Biochem. 114 : 64 – 70 .
   PubMedGoogle Scholar
• Moody , J. P. , and S. M. Allan . 1983 . Measurement of valproic acid in serum as the 4-bromophenacyl ester by high performance liquid chromatography . Clin. Chim. Acta 127 : 263 – 269 .
   PubMed Web of Science ®Google Scholar
• Nikolova-Damyanova , B. 1997 . Reversed phase HPLC: General principles and application to fatty acids and triacylglycerols . In Advances in Lipid Methodology-Four , ed. W. W. Christie . Dundee , Scotland : Oily Press .
   Google Scholar
• Nuhn , P. , L. Heine , and R. Benecke . 1985. Gaschromatographische Aktivitaetsbestimmung von Phospholipase A2. Zentralbl. Pharm. 124: 77–81.
   Google Scholar
• Pei , P. T. , W. G. Kossa , S. Ramachandran , and R. S. Henly . 1976 . High pressure reverse phase liquid chromatography of fatty acids p-bromophenacyl esters . Lipids 11 : 814 – 816 .
   PubMed Web of Science ®Google Scholar
• Pind , S. , and A. Kuksis . 1988 . Solubilization and assay of phospholipase A2 activity from rat jejunal brush-border membranes . Biochim. Biophys. Acta 938 : 211 – 221 .
   PubMed Web of Science ®Google Scholar
• Pungercar , J. , and I. Krizaj . 2007 . Understanding the molecular mechanism underlying the presynaptic toxicity of secreted phospholipases A2 . Toxicon 50 : 871 – 892 .
   PubMed Web of Science ®Google Scholar
• Puttmann , M. , H. Krug , E. von Ochsenstein , and R. Kattermann . 1993 . Fast HPLC determination of serum free fatty acids in the picomole range . Clin. Chem. 39 : 825 – 832 .
   PubMed Web of Science ®Google Scholar
• Richieri , G. V. , and A. M. Kleinfield . 1995 . Continuous measurement of phospholipase A2 activity using the fluorescent probe ADIFAB . Anal. Biochem. 229 : 256 – 263 .
   PubMedGoogle Scholar
• Rigoni , M. , P. Caccin , S. Gschmeissner , G. Koster , A. D. Postle , O. Rosseto , G. Schiavo , and C. Montecucco . 2005 . Equivalent effects of snake PLA2 neurotoxins and lysophospholipid–fatty acid mixtures . Science 310 : 1678 – 1680 .
   PubMed Web of Science ®Google Scholar
• Schmitt , M. , and M. Lehr . 2004 . HPLC assay with UV spectrometric detection for the evaluation of inhibitors of cytosolic phospholipase A2 . J. Pharm. Biomed. Anal. 35 : 135 – 142 .
   PubMed Web of Science ®Google Scholar
• Tojo , H. , T. Ono , and M. Okamoto . 1993 . Reverse-phase high performance liquid chromatographic assay of phospholipases: Application of spectrophotometric detection to rat phospholipase A2 isozymes . J. Lipid Res. 34 : 837 – 844 .
   PubMedGoogle Scholar
• Wilton , D. C. 1990 . A continuous fluorescence displacement assay for the measurement of phospholipase A2 and other lipases that release long-chain fatty acids . Biochem. J. 266 : 435 – 439 .
   PubMedGoogle Scholar