Quantification of RNase A and Its PEGylated Conjugates on Polymer-Salt Rich Environments Using UV Spectrophotometry

REFERENCES

• Abuchowski , A. , T. Van Es , N. C. Palczuk , and F. F. Davis . 1977 . Alteration of immunological properties of bovine serum albumin by covalent attachment of polyethylene glycol . J. Biol. Chem. 252 : 3578 – 3581 .
   PubMed Web of Science ®Google Scholar
• Benavides , J. , and M. Rito-Palomares . 2008 . Practical experiences from the development of aqueous two-phase processes for the recovery of high valuable biological products . J. Chem. Technol. Biotechnol. 83 : 133 – 142 .
   Web of Science ®Google Scholar
• Chai , X. , M. Jin , and C. Jiang . 1993 . Partition of proteins in aqueous two-phase systems containing charged dextran or hydrophobic PEG . Biotechnol. Techniques 7 : 373 – 378 .
   Google Scholar
• Cisneros-Ruiz , M. 2006 . Chromatographic separation of conjugates polymer – protein. PhD Diss., ITESM , Monterrey , Mexico .
   Google Scholar
• Cisneros-Ruiz , M. , K. Mayolo-Deloisa , T. M. Przybycien , and M. Rito-Palomares . 2009 . Separation of PEGylated from unmodified Ribonuclease A using sepharose media . Sep. Purif. Technol. 65 : 105 – 109 .
   Web of Science ®Google Scholar
• Delgado , C. , M. Malmsten , and J. M. Van Alstine . 1997 . Analytical partitioning of poly(ethylene glycol)-modified proteins . J. Chromatogr. B 692 : 263 – 272 .
   PubMedGoogle Scholar
• Fee , C. J. , and J. M. Van Alstine . 2006 . PEG-proteins: Reaction engineering and separation issues . Chem. Eng. Sci. 61 : 924 – 939 .
   Web of Science ®Google Scholar
• Frauenfelder , H. , G. Chen , J. Berendzen , P. W. Fenimore , H. Jansson , B. H. McMahon , et al. . 2009 . A unified model of protein dynamics . Proc. Natl. Acad. Sci. U.S.A. 106 : 5129 – 5134 .
   PubMed Web of Science ®Google Scholar
• Harris , J. M. , and R. B. Chess . 2003 . Effect of PEGylation on pharmaceuticals . Nat. Rev. Drug Discovery 2 : 214 – 221 .
   PubMed Web of Science ®Google Scholar
• Jentoft , N. , and D. G. Dearborn . 1979. Labeling of proteins by reductive methylation using sodium cyanoborohydride. J. Biol. Chem. 254: 4359–4365.
   PubMed Web of Science ®Google Scholar
• Matousek , J. , P. Pouckova , J. Soucek , and J. Skvor . 2002 . PEG chains increase aspermatogenic and antitumor activity of RNase A and BS-RNase enzymes . J. Controlled Release 82 : 29 – 37 .
   PubMed Web of Science ®Google Scholar
• Noble , J. , A. Knight , A. Reason , A. Di Matola , and M. Bailey . 2007 . A comparison of protein quantitation assays for biopharmaceutical applications . Mol. Biotechnol. 37 : 99 – 111 .
   PubMed Web of Science ®Google Scholar
• Pace , C. N. , F. Vajdos , L. Fee , G. Grimsley , and T. Gray . 1995 . How to measure and predict the molar absorption coefficient of a protein . Protein Sci. 4 : 2411 – 2423 .
   PubMed Web of Science ®Google Scholar
• Pesek , J. J. , M. T. Matyska , and G. B. Dawson . 2004 . Open tubular capillary electrochromatography of synthetic peptides on etched chemically modified columns . Anal. Chem. 76 : 23 – 30 .
   PubMed Web of Science ®Google Scholar
• Raines , R. T. 1998 . Ribonuclease A . Chem. Rev. 98 : 1045 – 1065 .
   PubMed Web of Science ®Google Scholar
• Rito-Palomares , M. 2004 . Practical application of aqueous two-phase partition to process development for the recovery of biological products . J. Chromatogr. B 807 : 3 – 11 .
   PubMed Web of Science ®Google Scholar
• Roberts , M. , M. Bentley , and J. Harris . 2002 . Chemistry for peptide and protein PEGylation . Adv. Drug Delivery Rev. 54 : 459 – 476 .
   PubMed Web of Science ®Google Scholar
• Rosenheck , K. , and P. Doty . 1961 . The far ultraviolet absorption spectra of polypeptide and protein solutions and their dependance on conformation . Biochemistry 47 : 1775 – 1785 .
   Google Scholar
• Snider , J. , C. Neville , L. Yuan , and J. Bullock . 1992 . Characterization of the heterogeneity of polyethylene glycol-modified superoxide dismutase by chromatographic and electrophoretic techniques . J. Chromatogr. 599 : 141 – 155 .
   PubMed Web of Science ®Google Scholar
• Sookkumnerd , T. , and J. T. Hsu . 2000 . Purification of PEG-protein conjugates by countercurrent distribution in aqueous two-phase systems . J. Liq. Chromatogr. Relat. Technol. 23 : 497 – 503 .
   Web of Science ®Google Scholar
• Veronese , F. M. , and G. Pasut . 2005 . PEGylation, successful approach to drug delivery . Drug Discovery Today 10 : 1451 – 1458 .
   PubMed Web of Science ®Google Scholar
• Zalipsky , S. 1995 . Functionalized poly(ethylene glycol) for preparation of biologically relevant conjugates . Bioconjugate Chem. 6 : 150 – 165 .
   PubMed Web of Science ®Google Scholar
• Zaslavsky , B. Y. 1995 . Aqueous Two-Phase Partitioning: Physical Chemistry and Bioanalytical Applications . New York , USA : Marcel Dekker, Inc .
   Google Scholar