125
Views
1
CrossRef citations to date
0
Altmetric
BIOANALYTICAL

Papain Purification Insights: Monitoring by Electrophoretic Approaches and MALDI-TOF Peptide Mass Fingerprint Analyses

, , &
Pages 2124-2137 | Received 29 Dec 2009, Accepted 21 Aug 2010, Published online: 12 Aug 2011

REFERENCES

  • Azarkan , M. , A. El Moussaoui , D. van Wuytswinkel , G. Dehon , and Y. Looze . 2003 . Fractionation and purification of the enzymes stored in the latex of Carica papaya . J. Chrom. B 790 : 229 – 238 .
  • Baines , B. S. , and K. Brocklehurst . 1979 . A necessary modification to the preparation of papain from any high-quality latex of Carica papaya and evidence for the structural integrity of the enzyme produced by traditional methods . Biochem. J. 177 : 541 – 548 .
  • Barrett , A. 2001. Proteolytic Enzymes: Nomenclature and Classification, Proteolytic Enzymes, A Practical Approach , eds. R. Beynon and J. S. Bond. Oxford : Oxford University Press.
  • Barrett , A. , N. D. Rawlings , and J. F. Woessner . 2004 . Handbook of Proteolytic Enzymes . London , UK : Academic Press .
  • Bradford , M. M. 1976 . A rapid and sensitive method for the quantitation of micrograms quantities of protein utilizing the principle of protein dye binding . Anal. Biochem. 72 : 248 – 254 .
  • Bruno , M. A. , S. A. Trejo , N. O. Caffini , and L. M. López . 2008 . Purification and characterization of hieronymain III. Comparison with other proteases previously isolated from Bromelia hieronymi Mez . Protein J. 27 : 426 – 433 .
  • Buttle , D. J. , A. A. Kembhavi , S. L. Sharp , R. T. Shute , D. H. Rich , and A. J. Barrett . 1989 . Affinity purification of the novel cysteine proteinase papaya proteinase IV, and papain from papaya latex . Biochem. J. 261 : 469 – 476 .
  • Damodaran , S. , T. D. Wood , P. Nagarajan , and R. A. Rabin . 2007 . Evaluating peptide mass fingerprinting-based protein identification . Geno. Prot. Bioinfo. 5 : 152 – 157 .
  • Domsalla , A. , and M. F. Melzig . 2008 . Occurrence and properties of proteases in plant lattices . Planta Med. 74 : 699 – 711 .
  • Dunn , M. J. 1989 . Initial planning. Electrophoretic analysis methods . In Protein Purification Methods: A Practical Approach , eds. R. Beynon and J. S. Bond . New York : IRL Press, Oxford University Press .
  • El Mossaoui , A. , M. Nijs , C. Paul , R. Witjens , J. Vincentelli , M. Azarkan , and Y. Looze . 2001 . Revisiting the enzymes stored in the laticifers of Carica papaya in the context of their possible participation in the plant defence mechanism . Cell. Mol. Life Sci. 58 : 556 – 570 .
  • Espin , N. , and M. N. Islam . 1998 . Stabilization of papain from papaya peels . Food Sci. Technol. Int. 4 : 179 – 187 .
  • Falkner , J. A. , M. Kachman , D. M. Veine , A. Walker , J. R. Strahler , and P. C. Andrews . 2007 . Validated MALDI-TOF/TOF mass spectra for protein standards . J. Am. Soc. Mass Spectrom. 18 : 850 – 855 .
  • Frank , A. M. , M. M. Savitski , M. N. Nielsen , R. A. Zubarev , and P. A. Pevzner . 2007 . De novo peptide sequencing and identification with precision mass spectrometry . J Proteome Res . 6 : 114 – 123 .
  • Gianazza , E. , and P. G. Righetti . 1980 . Facts and artifacts in isoelectric focusing . In Electrophoresis '79 , ed. B. J. Radola . Berlin : Walter de Gruyter .
  • Kimmel , J. R. , and E. L. Smith . 1954 . Crystalline papain. I. Preparation, specificity, and activation . J. Biol. Chem. 207 : 515 – 531 .
  • Matsumoto , K. , D. Yamamoto , H. Ohishi , K. Tomoo , T. Ishida , M. Inoue , T. Sadatome , K. Kitamura , and H. Mizuno . 1989 . Mode of binding of E-64-c, a potent thiol protease inhibitor, to papain as determined by X-ray crystal analysis of the complex . FEBS Lett. 245 : 177 – 180 .
  • Monge , A. , E. J. Barreiro , P. Huenchuñir , R. Pinzón , G. Mora , A. Núñez , et al. . 2008 . Functional foods: Reflections on an expanding market . Chemistry Int. 30 : 9 – 13 .
  • Morcelle , S. R. , S. A. Trejo , F. Canals , F. X. Avilés , and N. S. Priolo . 2004 . Funastrain c II, a cysteine endopeptidase purified from the latex of Funastrum clausum . Protein J. 23 : 205 – 215 .
  • Napper , A. D. , S. P. Bennett , M. Borowsku , M. B. Holdrige , M. J. C. Leonard , E. E. Rogers , Y. Duan , R. A. Laursen , B. Reinhold , and S. L. Shames . 1994 . Purification and characterization of multiple forms of the pineapple-stem-derived cysteine proteinases ananain and comosain . Biochem. J. 301 : 727 – 735 .
  • Neuhoff , V. , N. Arold , D. Taube , and W. Ehrhardt . 1988 . An improved procedure for staining of proteins following separation in polyacrylamide gels is described which utilizes the colloidal properties of Coomassie Brilliant Blue G-250 and R-250 . Electrophoresis 9 : 255 – 262 .
  • Obregón , W. D. , C. S. Liggieri , S. R. Morcelle , S. A. Trejo , F. X. Avilés , and N. S. Priolo . 2009. Biochemical and PMF MALDI-TOF analyses of two novel papain-like proteinases. Protein Pept. Lett. 16: 1323–1333.
  • Perkins , D. N. , D. J. C. Pappin , D. M. Creasy , and J. S. Cottrell . 1999 . Probability-based protein identification by searching sequence databases using mass spectrometry data . Electrophoresis 20 : 3551 – 3567 .
  • Priolo , N. , L. M. I. López , M. C. Arribére , C. L. Natalucci , and N. O. Caffini . 1991 . New purified plant proteinases for the food industry . Acta Aliment. 20 : 189 – 196 .
  • Schägger , H. , and G. von Jagow . 1987 . Tricine-sodium dodecyl sulfate polyacrilamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa . Anal. Biochem. 166 : 368 – 379 .
  • Takahashi , N. , Y. Yasuda , G. Kashiko , M. Tokiko , and T. Murachi . 1973 . Multiple molecular forms of stem bromelin . J. Biochem. 74 : 355 – 357 .

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.