Impact of dietary chitosan oligosaccharide and its effects on coccidia challenge in broiler chickens

References

• Abdel-Latif, M., H. M. Abdel-Haleem, and A. S. Abdel-Baki. 2016. “Anticoccidial Activities of Chitosan on Eimeria Papillata.” Parasitology Research 115: 2845–2852. doi:10.1007/s00436-016-5035-0.
   PubMed Web of Science ®Google Scholar
• Adedokun, S. A., K. M. Ajuwon, L. F. Romero, and O. Adeola. 2012. “Ileal Endogenous Amino Acid Losses: Response of Broiler Chickens to Fiber and Mild Coccidial Vaccine Challenge.” Poultry Science 91: 899–907. doi:10.3382/ps.2011-01777.
   PubMed Web of Science ®Google Scholar
• Adhikari, P., C. H. Lee, D. E. Cosby, N. A. Cox, and W. K. Kim. 2018. “Effect of Probiotics on Fecal Excretion, Colonization in Internal Organs and Immune Gene Expression in the Ileum of Laying Hens Challenged with Salmonella Enteritidis.” Poultry Science 98: 1235–1242. doi:10.3382/ps/pey443.
   Web of Science ®Google Scholar
• Allen, P. C., and R. H. Fetterer. 2002. “Interaction of Dietary Vitamin E with Eimeria Maxima Infections in Chickens.” Poultry Science 81: 41–48. doi:10.1093/ps/81.1.41.
   PubMed Web of Science ®Google Scholar
• Amer, O. S. O., M. A. Dkhil, W. M. Hikal, and S. Al-Quraishy. 2015. “Antioxidant and Anti-inflammatory Activities of Pomegranate (punica Granatum) on Eimeria Papillata-induced Infection in Mice.” Biomedical Research International 2015: 1–7.
   Web of Science ®Google Scholar
• Bai, W. Q., K. Y. Zhang, X. M. Ding, S. P. Bai, J. P. Wang, H. W. Peng, and Q. F. Zeng. 2019. “High Dietary Energy Content Increases Inflammatory Markers after Lipopolysaccharide Challenge in Meat Ducks.” Poultry Science 98: 164–171. doi:10.3382/ps/pey380.
   PubMed Web of Science ®Google Scholar
• Ballard, S. T., J. H. Hunter, and A. E. Taylor. 1995. “Regulation of Tight-junction Permeability during Nutrient Absorption across the Intestinal Epithelium.” Annual Review of Nutrition 15: 35–55. doi:10.1146/annurev.nu.15.070195.000343.
   PubMed Web of Science ®Google Scholar
• Chae, S. Y., M. Jang, and J. Nah. 2005. “Influence of Molecular Weight on Oral Absorption of Water Soluble Chitosans.” Journal of Control Release 102: 383–394. doi:10.1016/j.jconrel.2004.10.012.
   PubMed Web of Science ®Google Scholar
• Chen, Y. J., I. H. Kim, J. H. Cho, J. S. Yoo, Y. Wang, Y. Huang, H. J. Kim, and S. O. SHIN. 2009. “Effects of Chito-oligosaccharide Supplementation on Growth Performance, Nutrient Digestibility, Blood Characteristics and Immune Responses after Lipopolysaccharide Challenge in Weanling Pigs.” Livestock Science 124: 255–260. doi:10.1016/j.livsci.2009.02.006.
   Web of Science ®Google Scholar
• Cook, G. C. 1998. “Small Intestinal Coccidiosis and Emergent Clinical Problems.” Journal of Infection 16: 213–219. doi:10.1016/S0163-4453(88)97484-1.
   Web of Science ®Google Scholar
• Crini, G. 2005. “Recent Developments in Polysaccharide-based Materials Used as Absorbents in Wastewater Treatment.” Progress in Polymer Science 30: 38–70. doi:10.1016/j.progpolymsci.2004.11.002.
   Web of Science ®Google Scholar
• Cyktor, J. C., and J. Turner. 2011. “Interleukin-10 and Immunity against Prokaryotic and Eukaryotic Intracellular Pathogens.” Infectious Immunology 79: 2964–2973. doi:10.1128/IAI.00047-11.
   PubMed Web of Science ®Google Scholar
• Dalloul, R. A., T. W. Bliss, and Y. H. Hong. 2007. “Unique Responses of the Avian Macrophage to Different Species of Eimeria.” Molecular Immunology 44: 558–566. doi:10.1016/j.molimm.2006.02.004.
   PubMed Web of Science ®Google Scholar
• De Verdal, H., S. Mignon-Grasteau, C. Jeulin, E. L. Bihan-Duval, M. Leconte, S. Mallet, C. Martin, and A. Narcy. 2010. “Digestive Tract Measurements and Histological Adaptation in Broiler Lines Divergently Selected for Digestive Efficiency.” Poultry Science 89: 1955–1961. doi:10.3382/ps.2010-813.
   PubMed Web of Science ®Google Scholar
• Dimier-Poisson, I. H., D. T. Bout, and P. Quéré. 1999. “Chicken Primary Enterocytes: Inhibition of Eimeria Tenella Replication after Activation with Crude Interferon-γ Supernatants.” Avian Diseases 48: 617–624. doi:10.1637/7180-031604R.
   Web of Science ®Google Scholar
• Dkhil, M. A., D. Delic, and S. Al-Quraishy. 2013. “Goblet Cells and Mucin Related Gene Expression in Mice Infected with Eimeria Papillata.” The Scientific World Journal 2013: 1–6. doi:10.1155/2013/439865.
   Web of Science ®Google Scholar
• Du, A., S. Hu, and S. Wang. 2005. “Eimeria Tenella: Ginsenosides Enhanced Immune Response to the Immunization with Recombinant 5401 Antigen in Chickens.” Experimental Parasitology 111: 191–197. doi:10.1016/j.exppara.2005.07.006.
   PubMed Web of Science ®Google Scholar
• Fang, I. M., C. H. Yang, C. M. Yang, and M. S. Chen. 2013. “Chitosan Oligosaccharides Attenuates Oxidative Stress Related Retinal Degeneration in Rats.” PLoS One 8: 1–16. doi:10.1371/journal.pone.0077323.
   Web of Science ®Google Scholar
• Fasina, Y. O., P. S. Holt, E. T. Moran, R. W. Moore, D. E. Conner, and S. R. Mckee. 2008. “Intestinal Cytokine Response of Commercial Source Broiler Chicks to Salmonella Typhimurium Infection.” Poultry Science 87: 1335–1346. doi:10.3382/ps.2007-00228.
   PubMed Web of Science ®Google Scholar
• Gao, J., H. J. Zhang, S. H. Yu, S. G. Wu, I. Yoon, J. Quigley, Y. P. Gao, and G. H. Qi. 2008. “Effects of Yeast Culture in Broiler Diets on Performance and Immunomodulatory Functions.” Poultry Science 87: 1377–1384. doi:10.3382/ps.2007-00228.
   PubMed Web of Science ®Google Scholar
• Grenier, B., H. E. Schwartz-Zimmermann, S. Caha, W. D. Moll, G. Schatzmayr, and T. J. Applegate. 2015. “Dose-dependent Effects on Sphingoid Bases and Cytokines in Chickens Fed Diets Prepared with Fusarium Verticillioides Culture Material Containing Fumonisins.” Toxins 7: 1253–1272. doi:10.3390/toxins7051738.
   PubMed Web of Science ®Google Scholar
• Helander, I. M., E. L. Nurmiaho-Lassila, R. Ahvenainen, J. Rhoades, and S. Roller. 2001. “Chitosan Disrupts the Barrier Properties of the Outer Membrane of Gram-negative Bacteria.” International Journal of Food Microbiology 71: 235–244. doi:10.1016/S0168-1605(01)00609-2.
   PubMed Web of Science ®Google Scholar
• Hong, Y. H., H. S. Lillehoj, S. H. Lee, D. Park, and E. P. Lillehoj. 2006. “Molecular Cloning and Characterization of Chicken Lipopolysaccharide-induced TNF-alpha Factor (LITAF).” Developmental and Comparative Immunology 30: 919–929. doi:10.1016/j.dci.2005.06.002.
   PubMed Web of Science ®Google Scholar
• Huang, R. L., Y. L. Yin, G. Y. Wu, T. J. Zhang, L. L. Li, M. X. Li, Z. R. Tang, et al. 2005. “Effect of Dietary Oligochitosan Supplementation on Ileal Nutrient Digestibility and Performance in Broilers.” Poultry Science 84: 1383–1388. doi:10.1093/ps/84.9.1383.
   PubMed Web of Science ®Google Scholar
• Jeon, Y. J., P. J. Park, and S. K. Kim. 2001. “Antimicrobial Effect of Chitooligosaccharides Produced by Bioreactor.” Carbohydrate Polymers 44 (1): 71–76.
   Web of Science ®Google Scholar
• Johnson, R. W. 1997. “Inhibition of Growth by Pro-inflammatory Cytokines: An Integrated View.” Journal of Animal Science 75: 1244–1255. doi:10.2527/1997.7551244x.
   PubMed Web of Science ®Google Scholar
• Kettunen, H., K. Tiihonen, S. Peuranen, M. T. Saarinen, and J. C. Remus. 2001. “Dietary Betaine Accumulates in the Liver and Intestinal Tissue and Stabilizes the Intestinal Epithelial Structure in Healthy and Coccidia-infected Broiler Chicks.” Comparative Biochemistry Physiology 130: 759–769. doi:10.1016/S1095-6433(01)00410-X.
   Web of Science ®Google Scholar
• Khambualai, O., K. Yamauchi, S. Tangtaweewipat, and B. Cheva-Isarakul. 2008. “Effects of Dietary Chitosan Diets on Growth Performance in Broiler Chickens.” Journal of Poultry Science 45: 206–209. doi:10.2141/jpsa.45.206.
   Web of Science ®Google Scholar
• Khambualai, O., K. Yamauchi, S. Tangtaweewipat, and B. Cheva-Isarakul. 2009. “Growth Performance and Intestinal Histology in Broiler Chickens Fed with Dietary Chitosan.” British Poultry Science 50: 592–597. doi:10.1080/00071660903247182.
   PubMed Web of Science ®Google Scholar
• Kluth, H., K. Mehlhorn, and M. Rodehutscord. 2005. “Studies on the Intestine Section to Be Sampled in Broiler Studies on Precaecal Amino Acid Digestibility.” Archives of Animal Nutrition 59: 271–279. doi:10.1080/17450390500217058.
   PubMed Web of Science ®Google Scholar
• Koch, B. E., J. Stougaard, and H. P. Spaink. 2015. “Keeping Track of the Growing Number of Biological Functions of Chitin and Its Interaction Partners in Biomedical Research.” Glycobiology 25: 469–482. doi:10.1093/glycob/cwu134.
   PubMed Web of Science ®Google Scholar
• Kucharzik, T., S. V. Walsh, J. Chen, C. A. Parkos, and A. Nusrat. 2001. “Neutrophil Transmigration in Inflammatory Bowel Disease Is Associated with Differential Expression of Epithelial Intercellular Junction Proteins.” American Journal of Pathology 159: 2001–2009. doi:10.1016/S0002-9440(10)63051-9.
   PubMed Web of Science ®Google Scholar
• Lee, S. H., H. S. Lillehoj, S. I. Jang, D. K. Kim, C. Ionescu, and D. Bravo. 2010. “Effect of Dietary Curcuma, Capsicum, and Lenti- Nus on Enhancing Local Immunity against Eimeria Acervulina Infection.” Journal of Poultry Science 47: 89–95. doi:10.2141/jpsa.009025.
   Web of Science ®Google Scholar
• Lee, S. H., H. S. Lillehoj, S. I. Jang, K. W. Lee, D. Bravo, and E. P. Lillehoj. 2011. “Effects of Dietary Supplementation with Phytonutrients on Vaccine-stimulated Immunity against Infection with Eimeria tenella.” Veterinary Parasitology 181: 97–105. doi:10.1016/j.vetpar.2011.05.003.
   PubMed Web of Science ®Google Scholar
• Levine, N. D., K. N. Mehra, D. T. Clark, and I. J. Aves. 1960. “A Comparison of Nematode Egg Counting Techniques for Cattle and Sheep Feces.” American Journal of Veterinary Research 21: 511–515.
   PubMed Web of Science ®Google Scholar
• Li, X. J., X. S. Piao, S. W. Kim, P. Liu, L. Wang, Y. B. Shen, and S. C. Jung. 2007. “Effects Of Chito-oligosaccharide Supplementation on Performance, Nutrient Digestibility, and Serum Composition in Broiler Chickens.” Poultry Science 86: 1107–1114.
   PubMed Web of Science ®Google Scholar
• Lillehoj, H. S., C. H. Kim, C. L. Keeler, and S. Zhang. 2007. “Immunogenomic Approaches to Study Host Immunity to Enteric Pathogens.” Poultry Science 86: 1491–1500. doi:10.1093/ps/86.6.1196.
   PubMed Web of Science ®Google Scholar
• Lillehoj, H. S., and E. P. Lillehoj. 2000. “Avian Coccidiosis. A Review of Acquired Intestinal Immunity and Vaccination Strategies.” Avian Diseases 44: 408–425. doi:10.2307/1592556.
   PubMed Web of Science ®Google Scholar
• Lim, H. S., I. K. Paik, T. I. Sohn, and W. Y. Kim. 2006. “Effects of Supplementary Copper Chelates in the Form of Methionine, Chitosan and Yeast on the Performance of Broilers.” Asian Australian Journal of Animal Science 19: 1322–1327. doi:10.5713/ajas.2006.1322.
   Web of Science ®Google Scholar
• Liu, P., X. S. Piao, S. W. Kim, P. Liu, L. Wang, Y. B. Sheu, H. S. Lee, and S. Y. LI. 2008. “Effects of Chito-oligosaccharide Supplementation on Growth Performance, Nutrient Digestibility, Intestinal Morphology, and Fecal Shedding of Escherichia Coli and Lactobacillus in Weanling Pigs.” Journal of Animal Science 86: 2609–2618. doi:10.2527/jas.2007-0668.
   PubMed Web of Science ®Google Scholar
• Livak, K. J., and D. Schmittgen. 2001. “Analysis of Relative Gene Expression Data Using Real-time Quantitative PCR and the 2−δδct Method.” Methods 25: 402–408. doi:10.1006/meth.2001.1262.
   PubMed Web of Science ®Google Scholar
• Lynagh, G. R., M. Bailey, and P. Kaiser. 2000. “Interleukin-6 Is Produced during Both Murine and Avian Eimeria Infections.” Veterinary Immunology and Immunopathology 762: 89–102. doi:10.1016/S0165-2427(00)00203-8.
   Web of Science ®Google Scholar
• Mast, J., and B. M. Goddeeris. 1999. “Development of Immunocompetence of Broiler Chickens.” Veterinary Immunology and Immunopathology 70: 245–256. doi:10.1016/S0165-2427(99)00079-3.
   PubMed Web of Science ®Google Scholar
• Mcdougald, L. R. 2003. “Coccidiosis.” In Diseases of Poultry, edited by Y. M. Saif, 1001–1010. 11th ed. Ames: Iowa State Press.
   Google Scholar
• Metwaly, M. S., M. A. Dkhil, and S. Al-Quraishy. 2012. “The Potential Role of Phoenix Dactylifera on Eimeria Papillata-induced Infection in Mice.” Parasitology Research 111: 681–687. doi:10.1007/s00436-012-2938-2.
   PubMed Web of Science ®Google Scholar
• Mori, T., Y. Irie, S. I. Nishimura, S. Tokura, M. Matsuura, M. Okumura, T. Kadosawa, and T. Fujinaga. 1998. “Endothelial Cell Responses to Chitin and Its Derivatives.” Journal of Biomedical Materials Research Banner 43: 469–472. doi:10.1002/(SICI)1097-4636(199824)43:4<469::AID-JBM15>3.0.CO;2-9.
   PubMed Web of Science ®Google Scholar
• Ng, S. C., A. L. Hart, M. A. Kamm, A. J. Stagg, and S. C. Knight. 2009. “Mechanisms of Action of Probiotics: Recent Advances.” Inflammatory Bowel Diseases 15: 300–310. doi:10.1002/ibd.20602.
   PubMed Web of Science ®Google Scholar
• Nishimura, K., S. Nishimura, N. Nishi, I. Saiki, S. Tokura, and I. Azuma. 1984. “Immunological Activity of Chitin and Its Derivatives.” Vaccine 2: 93–99. doi:10.1016/S0264-410X(98)90039-1.
   PubMedGoogle Scholar
• Nrc. 1994. Nutrient Requirements of Poultry. 9th rev ed. Washington, DC: Natl. Acad. Press.
   Google Scholar
• O’Shea, C. J., T. Sweeney, M. B. Lynch, J. J. Callan, and J. V. O’Doherty. 2011. “Modification of Selected Bacteria and Markers of Protein Fermentation in the Distal Gastrointestinal Tract of Pigs upon Consumption of Chitosan Is Accompanied by Heightened Manure Odor Emissions.” Journal of Animal Science 89: 1366–1375. doi:10.2527/jas.2010-2922.
   PubMed Web of Science ®Google Scholar
• Okawa, Y., M. Kobayashi, S. Suzuki, and M. Suzuki. 2003. “Comparative Study of Protective Effects of Chitin, Chitosan and N-acetyl Chitohexaose against Pseudomonas Aeruginosa and Listeria Monocytogenes Infection in Mice.” Biological Pharmaceutical Bulletin 26: 902–904. doi:10.1248/bpb.26.902.
   PubMed Web of Science ®Google Scholar
• Olukosi, O. A., A. J. Cowieson, and O. Adeola. 2007. “Age-related Influence of a Cocktail of Xylanase, Amylase, and Protease or Phytase Individually or in Combination in Broilers.” Poultry Science 86: 77–86. doi:10.1093/ps/86.6.1196.
   PubMed Web of Science ®Google Scholar
• Papineau, A. M., D. G. Hoover, D. Knorr, and D. F. Farkas. 1991. “Antimicrobial Effect of Water Soluble Chitosans with High Hydrostatic Pressure.” Food Biotechnology 5: 45–57. doi:10.1080/08905439109549790.
   Web of Science ®Google Scholar
• Parker, J., E. O. Oviedo-Rondon, B. C. Clack, S. Clemente-Hernandez, J. Osborne, J. C. Remus, H. Kettunen, H. Makivuokko, and E. M. Pierson. 2007. “Enzymes as Feed Additives to Aid in Response against Eimeria Species in Coccidia-vaccinated Broilers Fed Corn-soybean Meal Diets with Different Protein Levels.” Poultry Science 86: 643–653. doi:10.1093/ps/86.6.1196.
   PubMed Web of Science ®Google Scholar
• Perez-Carbajal, C., D. Caldwell, M. Farnell, K. Stringfellow, S. Pohl, G. Casco, A. Pro-Martinez, and C. A. Ruiz-Feria. 2010. “Immune Response of Broiler Chickens Fed Different Levels of Arginine and Vitamin E to a Coccidiosis Vaccine and Eimeria Challenge. Poultry Science 89: 1870–1877. Produced by Bioreactor.” Carbohydrate Polymer 44: 71–76.
   Google Scholar
• Razdan, A., and D. Petterson. 1994. “Effect of Chitin and Chitosan on Nutrient Digestibility and Plasma Lipid Concentrations in Broiler Chickens.” British Journal of Nutrition 72: 277–288. doi:10.1079/BJN19940029.
   PubMed Web of Science ®Google Scholar
• Razdan, A., and D. Petterson. 1996. “Hypolipidaemic, Gastrointestinal and Related Responses of Broiler Chickens to Chitosans of Different Viscosity.” British Journal of Nutrition 76: 387–397. doi:10.1079/BJN19960044.
   PubMed Web of Science ®Google Scholar
• Razdan, A., D. Pettersson, and J. Pettersson. 1997. “Broiler Chicken Body Weights, Feed Intakes, Plasma Lipid and Small-intestinal Bile Acid Concentrations in Response to Feeding of Chitosan and Pectin.” British Journal of Nutrition 78: 283–291. doi:10.1079/BJN19970146.
   PubMed Web of Science ®Google Scholar
• Rhoades, J., G. Gibson, K. Formentin, M. Beer, and R. Rastall. 2006. “Inhibition of the Adhesion of Enteropathogenic Escherichia Coli Strains to HT-29 Cells in Culture by Chito-oligosaccharides.” Carbohydrate Polymer 64: 57–59. doi:10.1016/j.carbpol.2005.10.025.
   Web of Science ®Google Scholar
• Robbins, K. R., A. M. Saxton, and L. L. Southern. 2006. “Estimation of Nutrient Requirements Using Broken-line Regression Analysis.” Journal of Animal Science 84: 155–165. doi:10.2527/2006.8413_supplE155x.
   PubMedGoogle Scholar
• Saraiva, M., and A. O’Garra. 2010. “The Regulation of IL-10 Production by Immune Cells.” Nature Reviews Immunology 10: 170–181. doi:10.1038/nri2711.
   PubMed Web of Science ®Google Scholar
• Shao, Y., Y. Guo, and Z. Wang. 2013. “Beta-1,3/1,6-glucan Alleviated Intestinal Mucosal Barrier Impairment of Broiler Chickens Challenged with Salmonella Enterica Serovar Typhimurium.” Poultry Science 92: 1764–1773. doi:10.3382/ps.2013-03029.
   PubMed Web of Science ®Google Scholar
• Singla, A. K., and M. Chawla. 2001. “Chitosan: Some Pharmaceutical and Biological Aspects: An Update.” Journal of Pharmacy and Pharmacology 53: 1047–1067. doi:10.1211/0022357011777774.
   PubMed Web of Science ®Google Scholar
• Sudarshan, N. R., D. G. Hoover, and D. Knorr. 1992. “Antibacterial Action of Chitosan.” Food Biotechnology 6: 257–272. doi:10.1080/08905439209549838.
   Web of Science ®Google Scholar
• Suk, Y. O. 2004. “Interaction of Breed-by-chitosan Supplementation on Growth and Feed Efficiency at Different Supplementing Ages in Broiler Chickens.” Asian-Australasian Journal of Animal Science 17: 1705–1711. doi:10.5713/ajas.2004.1705.
   Web of Science ®Google Scholar
• Suzuki, T. 2013. “Regulation of Intestinal Epithelial Permeability by Tight Junctions.” Cell and Molecular Life Sciences 70: 631–659.
   PubMed Web of Science ®Google Scholar
• Swiatkiewicz, S., M. Swiatkiewicz, A. Arczewska-Wlosek, and D. Jozefiak. 2015. “Chitosan and Its Oligosaccharide Derivatives Chito-oligosaccharides as Feed Supplements in Poultry and Swine Nutrition.” Journal of Animal Physiology and Animal Nutrition 99: 1–12. doi:10.1111/jpn.12222.
   PubMed Web of Science ®Google Scholar
• Talebi, A., and G. Mulcahy. 2005. “Partial Protection against Eimeria Acervulina and Eimeria Tenella Induced by Synthetic Peptide Vaccine.” Experimental Parasitology 110: 342–348. doi:10.1016/j.exppara.2005.03.026.
   PubMed Web of Science ®Google Scholar
• Tan, J. Z., T. J. Applegate, S. Liu, Y. Guo, and S. D. Eicher. 2014. “Supplemental Dietary L-arginine Attenuates Intestinal Mucosal Disruption during a Coccidial Vaccine Challenge in Broiler Chickens.” British Journal of Nutrition 112: 1098–1109. doi:10.1017/S0007114514001846.
   PubMed Web of Science ®Google Scholar
• Tang, Z. R., Y. L. Yin, C. M. Nyachoti, R. L. Huang, T. J. Li, C. B. Yang, X. J. Yang. 2005. “Effect of Dietary Supplementation of Chitosan and Galacto mannanoligosaccharide on Serum Parameters and the Insulin-like Growth factor-I mRNA Expression in Early-weaned Piglets.” Domestic Animal Endocrinology 28: 430–441. doi:10.1016/j.domaniend.2005.02.003.
   Google Scholar
• Thongborisute, J., H. Takeuchi, H. Yamamoto, and Y. Kawashima. 2006. “Visualization of the Penetrative and Mucoadhesive Properties of Chitosan and Chitosan-coated Liposomes through the Rat Intestine.” Journal of Liposome Research 16: 127–141. doi:10.1080/08982100600680816.
   PubMed Web of Science ®Google Scholar
• Tokura, S., H. Tamura, and I. Azuma. 1999. “Immunological Aspects of Chitin and Chitin Derivatives Administered to Animals.” Experientia Supplementum 87: 279–292.
   PubMedGoogle Scholar
• Torzsas, T. L., C. W. C. Kendall, M. Sugano, Y. Iwamoto, and A. V. Rao. 1996. “The Influence of High and Low Molecular Weight Chitosan on Colonic Cell Proliferation and Aberrant Crypt Foci Development in CF1 Mice.” Food and Chemical Toxicology 34: 73–77. doi:10.1016/0278-6915(95)00083-6.
   PubMed Web of Science ®Google Scholar
• Waititu, S. M., A. Yitbarek, E. Matini, H. Echeverry, E. Kiarie, J. C. Rodriguez-Lecompte, and C. M. Nyachoti. 2014. “Effect of Supplementing Direct-fed Microbials on Broiler Performance, Nutrient Digestibilities, and Immune Responses.” Poultry Science 93: 625–635. doi:10.3382/ps.2013-03575.
   PubMed Web of Science ®Google Scholar
• Wang, X. W., Y. G. Du, X. F. Bai, and S. G. Li. 2003. “The Effect of Oligochitosan on Broiler Gut Flora, Microvilli Density, Immune Function and Growth Performance.” Acta Zoonutrimenta Sinica 15: 32–35.
   Google Scholar
• Williams, R. B. 2005. “Intercurrent Coccidiosis and Necrotic Enteritis of Chickens: Rational, Integrated Disease Management by Maintenance of Gut Integrity.” Avian Pathology 34: 159–180. doi:10.1080/03079450500112195.
   PubMed Web of Science ®Google Scholar
• Withanage, G., P. Wigley, P. Kaiser, P. Mastroeni, H. Brooks, C. Powers, R. Beal, P. Barrow, D. Maskell, and I. Mcconnell. 2005. “Cytokine and Chemokine Responses Associated with Clearance of a Primary Salmonella Enterica Serovar Typhimurium Infection in the Chicken and in Protective Immunity to Rechallenge.” Infectious Immunology 73: 5173–5182. doi:10.1128/IAI.73.8.5173-5182.2005.
   PubMed Web of Science ®Google Scholar
• Wu, Q. J., N. Liu, X. H. Wu, G. Y. Wang, and L. Lin. 2018. “Glutamine Alleviates Heat Stress-induced Impairment of Intestinal Morphology, Intestinal Inflammatory Response, and Barrier Integrity in Broilers.” Poultry Science 97: 2675–2683. doi:10.3382/ps/pey003.
   PubMed Web of Science ®Google Scholar
• Xiao, D., Z. Tang, Y. Yin, B. Zhang, X. Hu, Z. Feng, and J. Wang. 2013. “Effects of Dietary Administering Chitosan on Growth Performance, Jejunal Morphology, Jejunal Mucosal sIgA, Occluding Claudin-1 and TLR4 Expression in Weaned Piglets Challenged by Enterotoxigenic Escherichia Coli.” International Immunopharmacology 17: 670–676.
   PubMed Web of Science ®Google Scholar
• Xu, Y., B. Shi, S. Yan, T. Li, Y. Guo, and J. Li. 2013. “Effects of Chitosan on Body Weight Gain, Growth Hormone and Intestinal Morphology in Weaned Pigs.” Asian-Australasian Journal of Animal Science 26: 1484–1489.
   PubMed Web of Science ®Google Scholar
• Yun, C. H., H. S. Lillehoj, and K. D. Choi. 2000. “Eimeria Tenella Infection Induces Local Gamma-interferon Production and Intestinal Lymphocyte Subpopulation Changes.” Infectious Immunology 68: 1282–1288.
   PubMed Web of Science ®Google Scholar
• Zhang, M., T. Tan, H. Yuan, and C. Rui. 2003. “Insecticidal and Fungicidal Activities of Chitosan and Oligo-chitosan.” Journal Bioactive and Compatible Polymer 18: 391–400.
   Web of Science ®Google Scholar
• Zhu, L. X., Z. G. Song, H. Lin, and L. Yuan. 2003. “Effects of Chitosan on Growth Performance and Immune Function in Broiler Chickens.” China Feed 4: 15–17.
   Google Scholar