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Caryologia
International Journal of Cytology, Cytosystematics and Cytogenetics
Volume 51, 1998 - Issue 3-4
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Original Articles

Use of fluorochromes chromomycin A3 and DAPI to study constitutive heterochromatin and NORs in four species of bats (Phyllostomidae)

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Pages 265-278 | Received 02 Sep 1998, Accepted 12 Nov 1998, Published online: 31 Jan 2014

REFERENCES

  • Amemiya C.T. and Gold J.R., 1986.—Chromomycin A3 stains nucleolus organizer regions of fish chromosomes. Copeia, 1: 226–231.
  • Amemiya C.T. and Gold J.R., 1987.—Chromomycin staining of vertebrate chromosomes: enhancement of banding patterns by NaOH. Cytobios, 49: 147–152.
  • Auer H., Mayr B., Lambrou M. and Schleger W., 1987.—An extend chicken karyotype, including the NOR chromosome. Cytogenet. Cell Genet., 45: 218–221.
  • Baker R.J., Bass R.A. and Johnson A.M., 1979.—Evolutionary implications of chromosomal homology in four genera of Stenodermine bats (Phyllostomatidae: Chiroptera). Evolution, 33: 220–226.
  • Bella J.L., Fernández J.L. and Gosálvez J., 1995.—C-banding plus fluorochromes staining shows differences in C-, G-, and R-bands in human and mouse metaphase chromosomes. Genome, 38: 864–868.
  • Bella J.L. and Gosálvez J., 1994.—Banding human chromosomes using combined C-banding- fluorochrome staining technique. Biotechnic & Histochemistry, 69: 243–248.
  • Bernardi G., 1993.—The vertebrate genome: isochores and chromosomal bands. In: A.T. Sumner and A.C. Chandley (Eds) ‘Chromosomes Today’, vol. 11. Chapman & Hall, London.
  • Bickham J.W., 1987.—Chromosomal variation among seven species of lasiurine bats (Chiroptera: Vespertilionidae). J. Mammal., 68: 837–842.
  • Castro J., Vinas A., Sánchez L. and Martínez P., 1996.—Characterization of an atypical NOR site polymorphism in brown trout (Salmo trutta) with Ag- and CMA3- staining, and fluorescent in situ hybridization. Cytogenet. Cell Genet., 75: 234–239.
  • Comings D.E., Avelino E., Okada T.A. and Wyandt H.E., 1973.—The mechanism of C- and G-banding of chromosomes. Exp. Cell Res., 77: 469–493.
  • Ford C.E. and Hamerton J.L., 1956.—A colchicine hypotonic citrate squash sequence for mammalian chromosomes. Stain Technol., 31: 247–251.
  • Freshney R.I., 1986.—Animal cell culture. A practical approach. Washington D.C. Press, Oxford.
  • Galleni L., Stanyon R., Tellini A., Giordano C. and Santini L., 1992.—Karyology of the savi pine vole, Microtus savii (De Sélys-Longchomps, 1838) (Rodentia, Arvicolidae): G-, C, DA/DAPI-, and Alul-hands. Cytogenet. Cell Genet., 59: 290–292.
  • Howell W.M. and Black D. A., 1980.—Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia, 36: 1014–1015.
  • Kasahara S. and Dutrillaux B., 1983.—Chromosome handing patterns of four species of bats, with special reference to a case of X-autosome translocation. Ann. Genet., 26: 197–201.
  • Koehler M.R., Dehm D., Guttenbach M., Nanda I., Haaf T., Molina W.F., Galetti P.M.JR. and Schmid M., 1997.—Cytogenetics of the genus Leporinus (Pisces, Anostomidae). 1. Karyotype analysis, heterochromatin distribution and sex chromosomes. Chromosome Res., 5: 12–22.
  • Kopp E., Mayr B., Kalat M. and Schleger W., 1988.—Polymorphisms of NORs and heterochromatin in the horse and donkey. J. Heredity, 79: 332–337.
  • Lee M.R. and Elder F.F.B., 1980.—Yeast stimulation of bone marrow mitosis for cytogenetics investigations. Cytogenet. Cell Genet., 26: 36–40.
  • Mayr B., Geber G., Auer H., Kalat M. and Schleger W., 1986.—Heterochromatin composition and nucleolus organizer activity in four canid species. Can J. Genet. Cytol., 28: 744–753.
  • Mayr B., Krutzier J., Auer H., Kalat M. and Schleger W., 1987a.—NORs, heterochromatin, and R-bands in three species of Cervidae. J. Heredity, 78: 108–110.
  • Mayr B., Lambrou M., Kalat M., Schleger W. and Bigelbach A., 1990.—Characterization of heterochromatin by sequential counterstain enhanced fluorescence in three domestic bird species: Meleagris gallopavo, Columbia livia domestica and Anser anser L. J. Heredity, 81: 468–475.
  • Mayr B., Lambrou M., Schweizer D. and Kalat M., 1989.—An inversion polymorphism of a DA/DAPI-positive chromosome pair in Anas platyrhynchos (Aves). Cytogenet. Cell Genet., 50: 132–134.
  • Mayr B., Schweizer D., Mendelak M., Krutzier J., Schleger W., Kalat M. and Auer H., 1985.—Levels of conservation and variation of heterochromatin and nucleolus organizers in the Bovidae. Can J. Genet. Cytol., 27: 665–682.
  • Mayr B., Tesarik E., Auer H. and Burger H., 1987b.—Nucleolus-organizer regions and heterochromatin in three species of Bovidae. Genetica, 75: 207–212.
  • Meyne J., Baker R.J., Hobart H.H., Hsu T.C., Ryder O.A., Ward O.G., Wiley J.E., Wursterhill D., Yates T.L. and Moyzis R.K., 1990.—Distribution of non-telomeric sites of the (TTAGGG)n telomeric sequence in vertebrate chromosomes. Chromosoma (Berlin), 99: 3–10.
  • Morielle E. and Varella-Garcia M., 1988.—Variability of nucleolus organizer regions in phyllostomid bats. Rev. Brasil. Genet., 11: 853–871.
  • Ruedas L.A., Lee T.E.JR., Bickham W. and Schlitter D.A., 1990.—Chromosomes of five species of vespertilionid bats from Africa. J. Mammal., 71: 94–100.
  • Santos N. and Souza M.J., 1998.—Characterization of the constitutive heterochromatin of Carollia perspicillata (Phyllostomidae, Chiroptera) using the base-specific fluorochromes, CMA3 (GC) and DAPI (AT). Caryologia, 51: 51–60.
  • Schnedl W., Abraham R., Förster M. and Schweizer D., 1981.—Differential fluorescent staining of porcine heterochromatin by chromomycin A3/distamycin A/DAPI and D 287/170. Cytogenet. Cell Genet., 31: 249–253.
  • Schmid M., Enderle E., Schindler D. and Schempp W., 1989.—Chromosome banding and DNA replication patterns in bird karyotypes. Cytogenet. Cell Genet., 52: 139–146.
  • Schmid M., Feichtinger W., Weimer R., Mais C., Bolanos F. and León P. 1995.—Chromosome banding in Amphibia. XXI. Inversion polymorphism and multiple nucleolus organizer regions in Agalychnis callidryas (Anura, Hylidae). Cytogenet. Cell Genet., 69: 18–26.
  • Schmid M. and Guttenbach M., 1988.—Evolutionary diversity of reverse (R) fuorescent chromosome bands in vertebrates. Chromosoma (Berlin), 97: 101–114.
  • Schmid M., Steinlein C., Feichtinger W. and Poot M., 1993.—Chromosome banding in Amphibia. XVIII. Karyotype evolution and genomic size variation in Pleuroderma (Anura, Leptodactylidae). Cytogenet. Cell Genet., 62: 42–48.
  • Schweizer D., 1973.—Vergleichende Untersuchungen zur Langsdifferenzeirungen der Chromosomen von Vicia faba L. Verh. Naturfosch. Ges. Basil., 83: 1–75.
  • Schweizer D., 1976.—Reverse fluorescent chromosome handing with chromomycin and DAPI. Chromosoma, 58: 307–324.
  • Schweizer D., 1980.—Simultaneous fluorescent staining of R bands and specific heterochromatic regions (DA-DAPI bands) in human chromosomes. Cytogenet. Cell Genet., 27: 190–193.
  • Schweizer D., Ambros P., Andrle M., Rett A. and Fiedler W., 1979.—Demonstration of specific heterochromatin segments in the orangutan (Pongo pygmaeus) by a distamycin/DAPI double staining technique. Cytogenet. Cell Genet., 24: 7–14.
  • Sola L., Iaselli V., Rossi A.R., Rasch E.M. and Monaco P.J., 1992.—Cytogenetics of bisexual/unisexual species of Poecilia. II. Analysis of heterochromatin and nucleolar organizer regions in Poecilia mexicana by C-banding and DAPI, quinacrine, chromomycin A3 and silver staining. Cytogenet. Cell Genet., 60: 229–235.
  • Souza M.J. and Araujo M.C.P., 1990.—Conservative pattern of the G-bands and diversity of C-banding patterns and NORs in Stenodermatinae (Chiroptera-Phyllostomatidae). Rev. Brasil. Genet., 13: 255–268.
  • Sumner A.T., 1972.—A simple technique for demonstrating centromeric heterochromatin. Exp. Cell. Res., 75: 304–306.
  • Sumner A.T., Evans H.J. and Buckland R.A., 1973.—Mechanisms involved in the banding of chromosomes with quinacrine and Giemsa. I. The effects of fixation in methanol-acetic acid. Exp. Cell Res., 81: 214–222.
  • Ueda T., Irie S. and Kato Y., 1987.—Longitudinal differentiation of metaphase chromosomes of Indian muntjac as studied by restriction enzyme digestion, in situ hybridization with cloned DNA probes and distamycin A plus DAPI fluorescence staining. Chromosoma (Berlin), 95: 251–257.

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