Localization of rDNA loci by Fluorescent In Situ Hybridization in some wild orchids from Italy (Orchidaceae)

References

• Abbo , S. , Miller , T. E. , Reader , S. M. , Dunford , R. P. and King , I. P. 1994 . Detection of ribosomal DNA sites in lentil and chickpea by fluorescent in situ hybridisation . Genome , 37 : 713 – 716 .
   PubMed Web of Science ®Google Scholar
• Aceto , S. , Caputo , P. , Cozzolino , S. , Gaudio , L. and Moretti , A. 1999 . Phytogeny and evolution of Orchis and allied genera based on ITS DNA variation: morphological gaps and molecular continuity . Molecular Phylogenetics and Evolution , 13 : 67 – 76 .
   PubMed Web of Science ®Google Scholar
• Appels , R. and Dvorak , J . 1982 . Relative rates of divergence of spacers and gene sequences within the rDNA region of species in the Triticeae: implication for the maintenance of homogeneity of a repeated gene family . Theoretical Applied Genetics , 63 : 361 – 365 .
   PubMed Web of Science ®Google Scholar
• Arduino , P. , Cianchi , R. , Bullini , L. , Rossi , W. and Corrias , B. 1989 . “ Two hybrid zones between Orchis morio and Orchis longicornu: pattern and differences ” . In Proc. II Congr. European Society for Evolutionary Biology (E.S.E.B.), Rome 25–29 September, 1989 , 8 Universita di Roma “Tor Vergata” Pubbl .
   Google Scholar
• Bateman , R. M. , Pridgeon , A. M. and Chase , M.W. 1997 . Phylogenetics of subtribe Orchidi-nae (Orchidoideae, Orchidaceae) based on nuclear ITS sequences. 2. Infrageneric relationships and reclassification to achieve monophyly of Orchis sensu stricto . Lindlejana , 12 : 113 – 141 .
   Google Scholar
• Bianco , P. , D’Emerico , S. , Medagli , P. and Ruggi-Ero , L. 1991 . Polyploidy and aneuploidy in Ophrys, Orchis and Anacamptis (Orchidaceae) . Plant Systematics and Evolution , 178 : 235 – 245 .
   Web of Science ®Google Scholar
• D’emerico , S. , Bianco , P. and Medagli , P. 1992 . Karyological studies on Orchidaceae. Tribe Ophrydeae, subtribe Serapiadinae . Caryologia , 45 : 301 – 311 .
   Web of Science ®Google Scholar
• D’emerico , S. , Pignone , D. and Bianco , P. 1996a . Karyomorphological analyses and heterochromatin characteristic disclose phyletic relationships among 2n = 32 and 2n = 36 species of Orchis (Orchidaceae) . Plant Systematics and Evolution , 200 : 111 – 124 .
   Web of Science ®Google Scholar
• D’emerico , S. , Bianco , P. and Pignone , D. 1996b . Cytomorphological characterization of diploid and triploid individuals of Orchis x gennarii Keichenb. fil. (Orchidaceae) . Caryologia , 49 : 153 – 161 .
   Web of Science ®Google Scholar
• Fedoroff , N. V. 1979 . On spacers . Cell , 16 : 697 – 710 .
   PubMed Web of Science ®Google Scholar
• Galasso , I. , Blanco , A. , Katsiotis , A. , Pignone , D. and Heslop-Harrison , J. S. 1997 . Genomic organization and phylogenetic relationships in the genus Dasypyrum analysed by Southern and in situ hybridization of total genomic and cloned DNA probes . Chromosoma , 106 : 53 – 61 .
   PubMed Web of Science ®Google Scholar
• Galasso , I. , Schmidt , T. , Pignone , D. and Heslop-Harrison , J. S. 1995 . The molecular cytogenetics of Vigna unguiculata (L.) Walp: the physical organization and characterization of 18s–5.8s-25s rRNA genes, 5s rRNA genes, telomere-like sequences, and a family of centromeric repetitive DNA sequences . Theoretical and Applied Genetics , 91 : 928 – 935 .
   PubMed Web of Science ®Google Scholar
• Gerlach , W. L. and Bedbrook , J. R. 1979 . Cloning and characterization of ribosomal RNA genes from wheat and barley . Nucleic Acids Research , 7 : 1869 – 1885 .
   PubMed Web of Science ®Google Scholar
• Gerlach , W. L. and Dyer , T. A. 1980 . Sequence organization of the repeating units in the nucleus of wheat which contain 5 s rRNA genes . Nucleic Acids Research , 8 : 4851 – 4855 .
   PubMed Web of Science ®Google Scholar
• Heslop-Harrison , J. S. , Schwarzacher , T. , Anamtawat-Jonsoon , K. , Leitch , A. R. , Shi , M. and Leitch , I. J. 1991 . In situ hybridization with automated chromosome denaturation . Technique , 3 : 109 – 116 .
   Google Scholar
• Maluszynska , J. and Heslop-Harrison , J. S. 1993a . Physical mapping of rDNA in Brassica species . Genome , 36 : 774 – 781 .
   PubMed Web of Science ®Google Scholar
• Maluszynska , J. and Heslop-Harrison , J. S. 1993b . Molecular cytogenetics of the genus Arabidopsis: in situ localization of rDNA sites, chromosome numbers and diversity in centromeric heterochromatin . Annals of Botany , 71 : 479 – 484 .
   Web of Science ®Google Scholar
• Osuji , J. O. , Crouch , J. , Harrison , G. and Heslopharrison , J. S. 1998 . Molecular Cytogenetics ofMusa species, cultivars and hybrids: location of 18S-5.8S-25S and 5S rDNA and telomere-like sequences . Annals of Botany , 82 : 243 – 248 .
   Web of Science ®Google Scholar
• Pridgeon , A. M. , Bateman , R. M. , Cox , A. V. , Hapeman , J. R. and Chase , M. W. 1997 . Phylogenetics of subtribe Orchidinae (Orchidoideae, Orchidaceae) based on nuclear ITS sequences. 1. Intergeneric relationships and polyphyly of Orchis sensu lato . Lindleyana , 12 : 89 – 109 .
   Google Scholar
• Reddy , P. and Appels , R. 1989 . A second locus for the 5S multigene family in Secale L.: sequence divergencein two lineages of the family . Genome , 32 : 456 – 467 .
   Web of Science ®Google Scholar
• Rossi , W. , Corrias , B. , Arduino , P. , Cianchi , R. and Bullini , L. 1993 . Multilocus electrophoresis and European orchid systematics: the genus Orchis and related genera . Proc. XIV World Orchid Conf . 1993 , Glasgow . pp. 78 – 83 . H. M. S. O .
   Google Scholar
• Sastri , D. C. , Hilu , K. , Appels , R. , Lagudah , E. S. , Playford , J. and Baum , B. R. 1992 . An overview of evolution in plant 5S DNA . Plant Systematics and Evolution , 183 : 169 – 181 .
   Web of Science ®Google Scholar
• Taketa , S. , Harrison , G. E. and Heslop-Harrison , J. S. 1999 . Comparative physical mapping of the 5S and 18S-25S rDNA in nine wild Hordeum species and cytotypes . Theoretical and Applied Genetics , 98 : 1 – 9 .
   Web of Science ®Google Scholar
• Zoldos , V. , Papes , D. , Cerbah , M. , Panaud , O. , Besendorfer , V. and Slljak-Yakovlev , S. 1999 . Molecular-cytogenetic studies of ribosomal genes and heterochromatin reveal conserved genome organization among 11 Quercus species . Theoretical and Applied Genetics , 99 : 969 – 977 .
   Web of Science ®Google Scholar