838
Views
0
CrossRef citations to date
0
Altmetric
Regular Articles

Exploring the application of ELISA and slot blot as utility protein–protein interaction analysis tools

ORCID Icon, ORCID Icon, ORCID Icon & ORCID Icon

References

  • Berggård, T., Linse, S. & James, P. 2007. Methods for the detection and analysis of protein-protein interactions. Proteomics 7 (16): 2833–42. doi:10.1002/pmic.200700131.
  • Botha, M., Pesce, E.R. & Blatch, G.L. 2007. The Hsp40 proteins of Plasmodium falciparum and other apicomplexa: regulating chaperone power in the parasite and the host. Int J Biochem Cell Biol 39 (10): 1781–1803. doi:10.1016/j.biocel.2007.02.011.
  • Chakafana, G., Mudau, P.T., Zininga, T. & Shonhai, A. 2021. Characterisation of a unique linker segment of the Plasmodium falciparum cytosol localised Hsp110 chaperone. International Journal of Biological Macromolecules 180: 272–285. doi:10.1016/j.ijbiomac.2021.03.056.
  • Gitau, G.W., Mandal, P., Blatch, G.L., Przyborski, J. & Shonhai, A. 2012. Characterisation of the Plasmodium falciparum Hsp70-Hsp90 organising protein (PfHop). Cell Stress and Chaperones 17 (2): 191–202. doi:10.1007/s12192-011-0299-x.
  • Lebepe, C.M., Matambanadzo, P.R., Makhoba, X.H., Achilonu, I., Zininga, T. & Shonhai, A. 2020. Comparative characterization of Plasmodium falciparum Hsp70-1 relative to E. coli DnaK reveals the functional specificity of the parasite chaperone. Biomolecules 10 (6): 856.
  • Mabate, B., Zininga, T., Ramatsui, L., Makumire, S., Achilonu, I., Dirr, H.W. & Shonhai, A. 2018. Structural and biochemical characterization of Plasmodium falciparum Hsp70-x reveals functional versatility of its C-terminal EEVN motif. Proteins 86 (11): 1189–1201. doi:10.1002/prot.25600.
  • Makumire, S., Dongola, T.H., Chakafana, G., Tshikonwane, L., Chauke, C.T., Maharaj, T., Zininga, T. & Shonhai, A. 2021. Mutation of GGMP repeat segments of Plasmodium falciparum Hsp70-1 compromises chaperone function and Hop co-chaperone binding. International Journal of Molecular Sciences 22 (4): 2226. doi:10.3390/ijms22042226.
  • Miura, K. 2018. An overview of current methods to confirm protein-protein interactions. Protein Pept Lett 25 (8): 728–733. doi:10.2174/0929866525666180821122240.
  • Nguyen, H.H., Park, J., Kang, S. & Kim, M. 2015. Surface plasmon resonance: a versatile technique for biosensor applications. Sensors (Basel) 15 (5): 10481–10510. doi:10.3390/s150510481.
  • Shonhai, A., Boshoff, A. & Blatch, G.L. 2007. The structural and functional diversity of Hsp70 proteins from Plasmodium falciparum. Protein Science 16 (9): 1803–1818. doi:10.1110/ps.072918107.
  • Shonhai, A., Botha, M., de Beer, T., Boshoff, A. & Blatch, G.L. 2008. Structure-function study of a Plasmodium falciparum Hsp70 using three dimensional modelling and in vitro analyses. Protein & Peptide Letters 15 (10): 1117–1125.
  • Zininga, T., Achilonu, I., Hoppe, H., Prinsloo, E., Dirr, H.W. & Shonhai, A. 2015a. Overexpression, purification and characterisation of the Plasmodium falciparum Hsp70-z (PfHsp70-z) protein. PLoS One 10 (6): e0129445.
  • Zininga, T., Achilonu, I., Hoppe, H., Prinsloo, E., Dirr, H.W. & Shonhai, A. 2016. Plasmodium falciparum Hsp70-z, an Hsp110 homologue, exhibits independent chaperone activity and interacts with Hsp70-1 in a nucleotide-dependent fashion. Cell Stress and Chaperones 21 (3): 499–513. doi:10.1007/s12192-016-0678-4.
  • Zininga, T., Makumire, S., Gitau, G.W., Njunge, J.M., Pooe, O.J., Klimek, H., Scheurr, R., Raifer, H., Prinsloo, E., Przyborski, J.M., Hoppe, H. & Shonhai, A. 2015b. Plasmodium falciparum Hop (PfHop) interacts with the Hsp70 chaperone in a nucleotide-dependent fashion and exhibits ligand selectivity. PLoS One 10 (8): e0135326. doi:10.1371/journal.pone.0135326.
  • Zininga, T., Pooe, O.J., Makhado, P.B., Ramatsui, L., Prinsloo, E., Achilonu, I., Dirr, H. & Shonhai, A. 2017b. Polymyxin B inhibits the chaperone activity of Plasmodium falciparum Hsp70. Cell Stress and Chaperones 22 (5): 707–715. doi:10.1007/s12192-017-0797-6.
  • Zininga, T., Ramatsui, L., Makhado, P.B., Makumire, S., Achilinou, I., Hoppe, H., Dirr, H. & Shonhai, A. 2017a. (-)-Epigallocatechin-3-Gallate inhibits the chaperone activity of Plasmodium falciparum Hsp70 chaperones and abrogates their association with functional partners. Molecules 22: 12. doi:10.3390/molecules22122139.