137
Views
11
CrossRef citations to date
0
Altmetric
TECHNICAL NOTE

A six‐colour flow cytometric method for simultaneous detection of cell phenotype and apoptosis of circulating endothelial cells

, , , , , & show all
Pages 433-438 | Received 29 Sep 2008, Accepted 11 Nov 2008, Published online: 08 Jul 2009

References

  • Duda D. G., Cohen K. S., Scadden D. T., Jain R. K. A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor in human blood. Nature Protocols 2007; 2: 805–10
  • Strijbos M. H., Gratama J. W., Kraan J., Lamers C. H., den Bakker M. A., Sleijfer S. Circulating endothelial cells in oncology: pitfalls and promises. Br J Cancer 2008; 98: 1731–5
  • Goon P. K. Y., Boos C. J., Stonelake P. S., Blann A. D., Lip G. Y. H. Detection and quantification of mature circulating endothelial cells using flow cytometry and immunomagnetics beads: a methodological comparison. Thromb Haemost 2006; 96: 45–52
  • George F., Brouqui P., Boffa M. C., Drancourt M., Brisson C., Raoult D., Sampol J. Demonstration of Rickettsia conorii‐induced endothelial injury in vivo by measuring circulating endothelial cells, thrombomodulin, and von Willebrand factor in patients with Mediterranean spotted fever. Blood 1993; 82: 2109–16
  • Mancuso P., Burlini A., Pruneri G., Goldhirsch A., Martinelli G., Bertolini F. Resting and activated endothelial cells are increased in the peripheral blood of cancer patients. Blood 2001; 97: 3658–61
  • Mutunga M., Fulton B., Bullock R., Batchelor A., Gascoigne A., Gillespie J. I., Baudouin S. V. Circulating endothelial cells in patients with septic shock. AJRCCM 2001; 163: 195–200
  • Bull T. M., Golpon H., Hebbel R. P., Solovey A., Cool C. D., Tuder R. M., Geraci M. W., Voelkel N. F. Circulating endothelial cells in pulmonary hypertension. Thromb Haemost 2003; 90: 698–703
  • Del Papa N., Colombo G., Fracchiolla N., Moronetti L. M., Ingegnoli F., Maglione W., Comina D. P., Vitali C., Fantini F., Cortelezzi A. Circulating endothelial cells as a marker of ongoing vascular disease in systemic sclerosis. Arthritis Rheum 2004; 50: 1296–304
  • Nakatani K., Takeshita S., Tsujimoto H., Kawamura Y., Tokutomi T., Sekine I. Circulating endothelial cells in Kawasaki disease. Clin Exp Immunol 2003; 361: 536–40
  • Khan S. S., Solomon M. A., McCoy Jr J. P. Detection of circulating endothelial cells and endothelial progenitor cells by flow cytometry. Cytometry Part B. Clin Cytometry 2005; 64: 1–8
  • El Solh A. A., Akinnusi M. E., Baddoura F. H., Mankowski C. R. Endothelial cells apoptosis in obstructive sleep apnea. Am J Respir Crit Care Med 2007; 175: 1186–91
  • Bertolini F., Mancuso P., Shaked Y., Kerbel R. S. Molecular and cellular biomarkers for angiogenesis in clinical oncology. Drug Discovery Today 2007; 12: 806–12
  • Bertolini F. Chemotherapy and the tumour microenvironment: the contribution of circulating endothelial cells. Cancer Metastasis Rev 2008; 27: 95–101
  • Anonymous. World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. J Postgrad Med 2002; 48: 206–8
  • Whitby L., Granger W., Storie I., Goodfellow K., Sawle A., Reilly J. T., Barnett D. Quality control of CD4+ T‐lymphocyte enumeration: results from 9 years of the United Kingdom National External Quality Assessment Scheme for Immune Monitoring (1993–2001). Cytometry 2002; 50: 102–10
  • Körbling M., Reuben J. M., Gao H., Lee B‐N., Harris D. M., Cogdell D., Giralt S. A., Khouri I. F., Saliba R. M., Champlin R. E., Zhang W., Estrov Z. Recombinant human granulocyte‐colony‐stimulating factor‐mobilized and apheresis‐collected endothelial progenitor cells: a novel blood cells component for therapeutic vasculogenesis. Transfusion 2006; 46: 1795–802
  • Mariucci S., Rovati B., Bencardino K., Manzoni M., Danova M. Flow cytometric detection of circulating endothelial cells and endothelial progenitor cells in healthy subjects. Int J Lab Haematol. 2008, In press
  • Jacques N., Vimond N., Conforti R., Griscielli F., Lecluse Y., Laplanche A., Malka D., Vielh P., Farace F. Quantification of circulating mature endothelial cells using a whole blood four‐color flow cytometric assay. J Immunol Methods 2008; 15;337: 132–43
  • Redondo S., Hristov M., Gordillo‐Moscoso A. A., Ruiz E., Weber C., Tejerina T. High reproducible flow cytometric endothelial progenitor cell determination in human peripheral blood as CD34+/CD144+/CD3− lymphocyte sub‐population. J Immunol Methods 2008; 335: 21–7
  • Van Craenenbroeck E. M. F., Conraads V. M. A., Van Bockstaele D. R., Haine S. E., Vermeulen K., Van Tendeloo V. G., Vrints C. J., Hoymans V. J. Quantification of circulating endothelial progenitor cells: a methodological comparison of six flow cytometric approaches. J Immunol Methods 2008; 332: 31–40
  • McCarthy D. A., Macey M. G. Quality control in flow cytometry. Cytometric analysis of cell phenotype and function. Cambridge University Press, Cambridge 2001; 74
  • Wirezbwska A., Robak T., Krawczyńska A., Pluta A., Wrzesień‐Kuś A., Cebula B., Robak E., Smolewski P. Kinetics and apoptotic profile of circulating endothelial cells as prognostic factors for induction treatment failure in newly diagnosed acute myeloid leukemia patients. Ann Hematol 2008; 87: 97–106
  • Go R. S., Jobe D. A., Asp K. E., Callister S. M., Mathiason M. A., Meyer L. A., Bottner W. A., Cole C. E., Farner J. P., Frisby K. A. Circulating endothelial cells in patients with chronic lymphocytic leukemia. Ann Hematol 2008; 87: 369–73

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.