333
Views
0
CrossRef citations to date
0
Altmetric
Notes

A simplified HPLC-based method for measuring unconjugated bilirubin, bilirubin-monoglucuronide, bilirubin-diglucuronide, and delta-bilirubin in plasma with increased conjugated bilirubin

ORCID Icon, , &
Pages 64-67 | Received 07 Jun 2022, Accepted 04 Dec 2022, Published online: 19 Dec 2022

References

  • Vitek L, Ostrow JD. Bilirubin chemistry and metabolism; harmful and protective aspects. Curr Pharm Des. 2009;15(25):2869–2883.
  • Adachi Y, Murata N, Tsunasawa S, et al. Lysine 190 is a covalent bilirubin-binding amino acid in human Delta bilirubin. Int Hepatol Commun. 1996;5(4–5):282–288.
  • McDonagh AF, Palma LA, Lauff JJ, et al. Origin of mammalian biliprotein and rearrangement of bilirubin glucuronides in vivo in the rat. J Clin Invest. 1984;74(3):763–770.
  • Reed RG, Davidson LK, Burrington CM, et al. Non-resolving jaundice - bilirubin covalently attached to serum-albumin circulates with the same metabolic half-life as albumin. Clin Chem. 1988;34(10):1992–1994.
  • Kozaki N, Shimizu S, Higashijima H, et al. Significance of serum Delta-bilirubin in patients with obstructive jaundice. J Surg Res. 1998;79(1):61–65.
  • Higashijima H, Yamashita H, Makino I, et al. Significance of serum Delta bilirubin during obstructive jaundice in dogs. J Surg Res. 1996;66(2):119–124.
  • Brett EM, Hicks JM, Powers DM, et al. Delta bilirubin in serum of pediatric patients: correlations with age and disease. Clin Chem. 1984;30(9):1561–1564.
  • Westwood A. The analysis of bilirubin in serum. Ann Clin Biochem. 1991;28(2):119–130.
  • Doumas BT, Wu TW, Jendrzejczak B. Delta bilirubin: absorption spectra, molar absorptivity, and reactivity in the diazo reaction. Clin Chem. 1987;33(6):769–774.
  • Wu TW, Dappen GM, Powers DM, et al. The Kodak Ektachem clinical chemistry slide for measurement of bilirubin in newborns: principles and performance. Clin Chem. 1982;28(12):2366–2372.
  • Davis AR, Rosenthal P, Escobar GJ, et al. Interpreting conjugated bilirubin levels in newborns. J Pediatr. 2011;158(4):562–565 e1.
  • Ye W, Rosenthal P, Magee JC, et al. Factors determining Delta-Bilirubin levels in infants with biliary atresia. J Pediatr Gastroenterol Nutr. 2015;60(5):659–663.
  • Greene DN, Liang J, Holmes DT, et al. Neonatal total bilirubin measurements: still room for harmonization. Clin Biochem. 2014;47(12):1112–1115.
  • Osawa S, Sugo S, Yoshida T, et al. An assay for separating and quantifying four bilirubin fractions in untreated human serum using isocratic high-performance liquid chromatography. Clin Chim Acta. 2006;366(1–2):146–155.
  • Lauff JJ, Kasper ME, Wu TW, et al. Isolation and preliminary characterization of a fraction of bilirubin in serum that is firmly bound to protein. Clin Chem. 1982;28(4 Pt 1):629–637.
  • Hertz H, Dybkaer R. Molar absorption coefficients for bilirubins in adult and infant serum with determination of an isosbestic point. Scand J Clin Lab Invest. 1972;29(2):217–230.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.