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Original

Removal of endogenous ligands from a high-affinity antiserum for radioimmunoassay

Pages 533-541 | Received 26 May 1978, Accepted 06 Mar 1979, Published online: 14 Feb 2011

References

  • Abraham G. E. Solid-phase radioimmunoassay of estradiol-17β. J. din. Endocr. 1969; 29: 866
  • Alexander N. M., Jennings J. F. Radioimmunoassay of serum triiodothyronine on small, reusable Sephadex columns. Clin. Chem. 1974; 20: 1353
  • Auletta F. J., Caldwell B. V., Hamilton G. L. Androgens: Testosterone and dihydrotestosterone. Methods of Hormone Radioimmunoassay, B. M. Jaffe, H. R. Behrman. Academic Press, New York and London 1974; 359
  • Dunn R. T., Foster L. B. Radioimmunoassay of thyroxine in unextracted serum, by a single-antibody technique. Clin. Chem. 1973; 19: 1063
  • Ekins R. P. Basic principles and theory. Br. med. Bull. 1974; 30: 3
  • Fyhrquist F., Wallenius M. Improvement of antisera against polypeptide hormones. Nature (Lond.) 1975; 254: 82
  • Hillier S. G., Cole E. N., Groom G. V., Boyns A. R., Cameron E. H.D. Effect of active immunisation against testosterone-3-BSA on circulating levels of testosterone, LH, prolactin and testosterone antibody titre in the male rat. Steroids 1973; 22: 227
  • Kruse V. Production and evaluation of high-quality thyroxine antisera for use in radioimmunoassay. Scand. J. clin. Lab. Invest. 1976; 36: 95
  • Kruse V. A method for removal of endogenous ligands from high-affinity antibodies to be used in radioimmunoassay. Abstracts 2nd European Congress on Clinical Chemistry. Prague 1976; 57
  • Kruse V. Dissociation rate constants and fractional binding of tracer estimated for three antibody populations in unstripped and stripped antiserum. Scand. J. din. Lab. Invest. 1979; 39: 215
  • Kruse V. Selective removal of fast-dissociating antibodies from a high-affinity antiserum. Molec. Immun., In press
  • Kruse V., Lind O. A rapid and precise sequential saturation radioimmunoassay for thyroxine. Scand. J. din. Lab. Invest. 1977; 37: 149
  • Nieschlag E., Usadel K. H., Schwedes U., Kley H. K., Schöffling K., Krüskemper H. L. Alterations in testicular morphology and function in rabbits following active immunization with testosterone. Endocrinology 1973; 92: 1142
  • Niswender G. D. Steroid Immunoassay, E. H.D. Cameron, S. G. Hillier, K. Griffiths. Alpha Omega Publishing Ltd., CardiffUK 1975; 128
  • Oliver L. K., Cano C. Removal of an endogenous antigen from an antibody to increase its effective affinity constant, as illustrated by triiodothyronine assay. Clin. Chem 1977; 23: 2039
  • O'Neill S. P., Robb T. E. Hapten concentrations in the circulation of animals actively immunized with hapten-protein conjugates. J. Immun. 1976; 116: 363
  • Pratt J. J., Woldring M. G. Radioimmunoassay specificity and the 'first-come, first-served effect'. Clin. Chim. Acta 1976; 68: 87
  • Rodbard D. Statistical quality control and routine data processing for radioimmunoassays and immunoradiometric assays. Clin. Chem. 1974; 20: 1255
  • Seth J., Rutherford F. J., McKenzie I. Solid-phase radioimmunossay of thyroxine in untreated serum. Clin. Chem. 1975; 21: 1406
  • Skrabanek P., Kirrane J., Powell D. Apparent improvement of antisera for radioimmunoassay by treatment with sodium iodide. J. Immun. Meth. 1977; 16: 331

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